Search found 4031 matches

by mrsonchus
Sat Apr 17, 2021 11:57 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Hi John, glad to see you are still having fun and working hard over there in Cumbria. Thanks for the post. Hi Roldorf, yes still going strong! Today I mounted a few test-slides of 'broad-bean' (vicia.faba) edible seeds in LS to get a view of the embryonic shoot and root structures. This went quite ...
by mrsonchus
Sat Apr 17, 2021 11:40 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Also I have been using histoclear 2 and omnimount. I must say I am very pleased with those two. A well-matched combination. I've tried this combo in the past, in fact I still have both in the lab. I reverted back to Histoclear-original a few years back however, combined with 'Histomount' as a repla...
by mrsonchus
Sat Apr 17, 2021 8:52 pm
Forum: Specimens, samples and slides
Topic: Which staining?
Replies: 1
Views: 46

Re: Which staining?

Hi, this is almost certainly the classic safranin + fast-green stain combination. It certainly looks like it, although there's a certain 'greyness' to the cytoplasmic stain here. Protocol? An enormous subject dependant upon many, many factors - you'll need to have a good look around the internet for...
by mrsonchus
Fri Apr 16, 2021 7:35 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Thanks John. I managed to get a few better cuts but after a few days in safranin and washed with water, all the specimens peel off this cheap slide the second it touches the first alcohol grade. I haven't coated any of these slides with albumen! I got some superfrost + on order and I guess I'll sta...
by mrsonchus
Fri Apr 16, 2021 7:13 pm
Forum: Microscopy accessories
Topic: Disposable blades with Cambridge rocking microtome, anybody?
Replies: 14
Views: 532

Re: Disposable blades with Cambridge rocking microtome, anybody?

Hi, very nice cuts to my eyes!

The rocker is a fine little machine - your sections are certainly as good as any of the best hand-sections you'll see. It all depends on what you want to achieve as far as infiltration and embedding goes.

Congratulations, things are going well!
by mrsonchus
Sun Apr 11, 2021 3:44 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

I don't have any pictures handy right now but the ribbon has a little of that accordion crinkling going on. Going to try to shrink the presentation angle a little bit see if that helps. I rigged up a Sir all ralph knife adapter for the paraffin sectioning kit to hold Feather single edge razors, whi...
by mrsonchus
Sat Apr 10, 2021 8:57 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

I have noticed quite a bit of compression using paraplast with some FAA-preserved trametes. It's a soft wax to be sure, and my cutting room is closer to 75 F where I think this paraplast really prefers to be cut around 65 F. Trametes are pretty woody in my case, though, so maybe I will try some dif...
by mrsonchus
Thu Apr 08, 2021 9:41 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Thanks Jim, dtsh, pippo1234, Pete and mazo4033, that's really nice to know, it's great to be able to share adventures, but I sometimes let my posts get a little dense I think, I have such an interest and enjoyment of Botany and microscopy that I'm inclined to get a little carried away... Thanks for ...
by mrsonchus
Wed Apr 07, 2021 9:09 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Thanks deBult, very nice links. That site is jammed with interesting exploits and no trouble to set the Google translator onto it! This site is where I started with microscopy back in 2015 - feels like home here, and the amount of help and encouragement I've benefited from over those years from the ...
by mrsonchus
Wed Apr 07, 2021 9:01 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Super informative post, John. Thank you! Ideally this will be up for years (decades?) to come and a guide for aspiring microscopists. Thanks PeteM, very kind to say such - much appreciated. It's always great to know folks may have taken a little pleasure from a post, as I do everyday reading throug...
by mrsonchus
Tue Apr 06, 2021 11:25 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Hi Bob, pleased to know you find them interesting - I began to wonder if it was worth the effort of posting to be honest, so little interest outside of equipment and pond-water at the moment. Understandable though as the live-mounts are so accessible. Most folk aren't as time-rich as I am, even with...
by mrsonchus
Sun Apr 04, 2021 11:08 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 797

Re: [HELP] - Hayear Cameras Setup & Software

ToupCams are able to use all functions with the actual ToupView version (i.e. the genuine and original version) whilst others are just using the ToupView with basic webcam capture program functionality. The same funtionality is available with most webcam capture programs such as Windows own camera s...
by mrsonchus
Sun Apr 04, 2021 3:24 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Re: Slide Making with 61°C Wax

Here are some first-look slides from the other day, a selection of different staining and pre-treatments were tried here to see what worked, and of course what I liked.... Some better individual slide-images to follow. 1000px WS_first slide set.jpeg Here's a safranin + alcian-blue stained LS of a mo...
by mrsonchus
Sun Apr 04, 2021 2:19 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 26
Views: 1178

Slide Making with 61°C Wax

Hi all, some will have seen my earlier post re making slides from sectioned Lichen wherein I mentioned that there had been a small amount of tissue-folding during sectioning..... I decided to try a different paraffin embedding wax in an attempt to mitigate and ideally eliminate this phenomenon which...
by mrsonchus
Sun Apr 04, 2021 12:27 am
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 797

Re: [HELP] - Hayear Cameras Setup & Software

The functions are built-into the camera's own software, accessed via the remote control or the buttons on the camera. This menu system will only be usable and visible with HDMI connected screens/monitor. To be controlled from a PC (via USB) the operating sytem of the PC needs the presence of driver-...
by mrsonchus
Sat Apr 03, 2021 7:13 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 797

Re: [HELP] - Hayear Cameras Setup & Software

In your image there is no device shown as recognized in the device list. Are you connected only with USB and is the camera's micro-SD card slot empty?
by mrsonchus
Sat Apr 03, 2021 4:21 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 797

Re: [HELP] - Hayear Cameras Setup & Software

Hi, I too have such a camera, not however a Hayear branded, and unable to use any Toupview re-badge as such. However what I do know is that these cameras, when attached to you PC via USB, have no ability to change the settings of the camera. The settings are only available via the camera's own built...
by mrsonchus
Sat Apr 03, 2021 11:31 am
Forum: Specimens, samples and slides
Topic: My first attempt at using a hand microtome
Replies: 8
Views: 421

Re: My first attempt at using a hand microtome

Very nice start!
A book on plant anatomy would add many layers of interest to your sectioning. The more you look over such revealed details the more the complexity adds to the pleasure and mystery!

Congratulation for these fine sections & images!
by mrsonchus
Sat Mar 27, 2021 11:52 am
Forum: Pictures and Videos
Topic: Aspergillus - Prepared Amscope Slide
Replies: 2
Views: 195

Re: Aspergillus - Prepared Amscope Slide

Must agree, a nice interesting image. Thanks for sharing.
by mrsonchus
Sat Mar 27, 2021 11:20 am
Forum: Identification help
Topic: Any idea what this part of the flower is?
Replies: 11
Views: 293

Re: Any idea what this part of the flower is?

Hi, not even certain it's an orchid - have any parts of the flower's interior been broken away? The pollen is certain though. The protrusions look a lot like 'hairs' as they don't seem to have any elaborated structure to hold 'pollinia' - bundles of pollen as presented by orchids rather than discret...
by mrsonchus
Thu Mar 25, 2021 4:42 pm
Forum: Resources (online, books etc.)
Topic: Cambridge Rocking Microtome Manual
Replies: 3
Views: 3536

Re: Cambridge Rocking Microtome Manual

Have you had a look at a short video I made a while ago of my use of the 'rocker'?
by mrsonchus
Wed Mar 24, 2021 11:17 pm
Forum: My microscope
Topic: 7X-45X Dual Lit 6W LED Trinocular Stereo
Replies: 7
Views: 394

Re: 7X-45X Dual Lit 6W LED Trinocular Stereo

The problem as experienced, that is of the camera's image being only a small part of the total image seen through the eyepieces is a very well-known and common one. Simply-stated perhaps, it's a matter of the image produced at the eyepieces and 'up the photo-tube' being far larger than the size of t...
by mrsonchus
Mon Mar 22, 2021 5:24 pm
Forum: Pictures and Videos
Topic: Just Some Pics With Stereo Microscope
Replies: 6
Views: 188

Re: Just Some Pics With Stereo Microscope

Cracking 'scope like one I had a while back.
by mrsonchus
Mon Mar 22, 2021 5:19 pm
Forum: My microscope
Topic: 7X-45X Dual Lit 6W LED Trinocular Stereo
Replies: 7
Views: 394

Re: 7X-45X Dual Lit 6W LED Trinocular Stereo

Congratulations! Exciting times ahead for you! I had one of these a while back and it served me very well indeed. Eventually I was able to moved over to Nikon but this little beauty is superb value for money given it's very nice performance, in my experience of it. Look forward to seeing how you get...
by mrsonchus
Mon Mar 22, 2021 5:14 pm
Forum: Pictures and Videos
Topic: Just Some Pics With Stereo Microscope
Replies: 6
Views: 188

Re: Just Some Pics With Stereo Microscope

Very nice images, so colourful and detailed. I'd say these were pretty good for a stereo 'scope. Is the 'scope a Greenough type?
by mrsonchus
Thu Mar 18, 2021 5:24 pm
Forum: Camera systems and imaging
Topic: Will camera sensor overheat in Live View?
Replies: 6
Views: 379

Re: Will camera sensor overheat in Live View?

Hi Ed, I as you'll know I use a DSLR in liveview with my BX50, and have always had the same reservation. As I replace one DSLR (Canon 1200D) with another (Canon D200) at significant cost to my already broken wallet, I find that I become ever more aware of this, although I don't have an answer that I...
by mrsonchus
Thu Mar 18, 2021 4:49 pm
Forum: Illumination Techniques
Topic: Copepods - 3 illuminations
Replies: 3
Views: 266

Re: Copepods - 3 illuminations

Not my area at all, but I'm always interested in the different perspectives of alternative lighting. All three are great images simply put, but the variety of information that they show is really interesting! I'm particularly impressed with the middle image and it's superb capture of orientation, mu...
by mrsonchus
Sun Mar 14, 2021 11:09 am
Forum: Microscopes and optics
Topic: Interesting article: Getting the best out of a basic microscope
Replies: 7
Views: 350

Re: Interesting article: Getting the best out of a basic microscope

Superb - what a clean, clear and eminently useful web-site that one is.
The article as said is a nice fresh perspective and certainly very encouraging for the earlier stages of microscopy.

Thanks for this link, in particular, if you're recently new to microscopy give this one a look!
by mrsonchus
Wed Mar 10, 2021 4:38 pm
Forum: Home-made microscope adaptations
Topic: Have 3d printer, will print.
Replies: 30
Views: 1812

Re: Have 3d printer, will print.

They definitely look fit-for-purpose! Good images too.
by mrsonchus
Tue Mar 09, 2021 7:37 pm
Forum: Pictures and Videos
Topic: Rockport, Texas Diatoms
Replies: 19
Views: 623

Re: Rockport, Texas Diatoms

Agree with Bob there - the live diatoms are the ones I prefer, so much more to 'decode' and learn. This is the first time I've ever heard of diatoms in this mucilaginous-tube arrangement. How fascinating indeed!

The image quality, well, what can I add, simply excellent. More please..... A real treat!