Search found 4039 matches

by mrsonchus
Tue Apr 20, 2021 12:25 am
Forum: Microscopes and optics
Topic: Can't focus new 60X objective
Replies: 17
Views: 216

Re: Can't focus new 60X objective

.............deletion for brevity only.................. There is a Chinese infinity corrected planachro 60X .80 objective , probably based on an Olympus design. It works on a Microstar IV and can be easily made parfocal but I found the contrast and resolution of that objective a little too low, co...
by mrsonchus
Tue Apr 20, 2021 12:14 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Oh, here are a few images of some slides I mounted unstained, for use in polarization, phase-contrast etc microscopy. This will often reveal or clarify detail that is invisible or uncertain in stained slides. For phase-contrast I find that well mounted unstained sections are far superior to trying t...
by mrsonchus
Mon Apr 19, 2021 11:36 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

even better than egg white and glycerin i bet! meanwhile my superfrost + arrived all broken and my fast green is actually green lake-- useless! Congrats on getting any slides made. It is a lot of heartache. Urghhhh - that's bad luck. Here in the U.K. over the last 5-6 years I have never had a singl...
by mrsonchus
Mon Apr 19, 2021 11:29 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Thank you for the response re: differentiation of safranin O. That's an impressive array of sections you've produced and the close-ups look really good. There will be a lot of people on the forum interested to see the results after staining I'm sure, and any further information on your experience w...
by mrsonchus
Mon Apr 19, 2021 8:04 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Yes I have found this to be the case. The formulation with mordant and intensifier does appear to differentiate more effectively than the 'plain' formulation. By this I mean that the differentiation (essentially controllable and hopefully selective removal of safranin) appears to be faster, greater ...
by mrsonchus
Mon Apr 19, 2021 10:46 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Which stain was used for the slide of Root Tip? The first and last images above of the embryonic (hence the abscence of mitosis) root-tip is a safranin/fast-green one, with the addition of orange-g which as well as acting as a differentiator for safranin, adds to the staining protocol to create a r...
by mrsonchus
Mon Apr 19, 2021 10:43 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

More terrific pictures John, thank you! But you've done it now... "enhanced" safranin formula??? Did I miss something? Please elaborate. Tom W. Hi, the additives are borrowed fromJohansen's protocol - Sodium-acetate at 1% (W/V) as an 'intensifier' and Formalin at 2% (V/V) as a mordant - they seem t...
by mrsonchus
Mon Apr 19, 2021 10:32 am
Forum: Specimens, samples and slides
Topic: Which staining?
Replies: 3
Views: 119

Re: Which staining?

Hi, no way to tell if this is Sass' method I'm afraid. However, Sass' method is pretty simple, with no real additives, and does give good results - I've used it hundreds of times for plant tissue. Essentially Sass' pethod is: (slides having been de-waxed, cleared and rehydrated to de-ionized water) ...
by mrsonchus
Sat Apr 17, 2021 11:57 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Hi John, glad to see you are still having fun and working hard over there in Cumbria. Thanks for the post. Hi Roldorf, yes still going strong! Today I mounted a few test-slides of 'broad-bean' (vicia.faba) edible seeds in LS to get a view of the embryonic shoot and root structures. This went quite ...
by mrsonchus
Sat Apr 17, 2021 11:40 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Also I have been using histoclear 2 and omnimount. I must say I am very pleased with those two. A well-matched combination. I've tried this combo in the past, in fact I still have both in the lab. I reverted back to Histoclear-original a few years back however, combined with 'Histomount' as a repla...
by mrsonchus
Sat Apr 17, 2021 8:52 pm
Forum: Specimens, samples and slides
Topic: Which staining?
Replies: 3
Views: 119

Re: Which staining?

Hi, this is almost certainly the classic safranin + fast-green stain combination. It certainly looks like it, although there's a certain 'greyness' to the cytoplasmic stain here. Protocol? An enormous subject dependant upon many, many factors - you'll need to have a good look around the internet for...
by mrsonchus
Fri Apr 16, 2021 7:35 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Thanks John. I managed to get a few better cuts but after a few days in safranin and washed with water, all the specimens peel off this cheap slide the second it touches the first alcohol grade. I haven't coated any of these slides with albumen! I got some superfrost + on order and I guess I'll sta...
by mrsonchus
Fri Apr 16, 2021 7:13 pm
Forum: Microscopy accessories
Topic: Disposable blades with Cambridge rocking microtome, anybody?
Replies: 14
Views: 573

Re: Disposable blades with Cambridge rocking microtome, anybody?

Hi, very nice cuts to my eyes!

The rocker is a fine little machine - your sections are certainly as good as any of the best hand-sections you'll see. It all depends on what you want to achieve as far as infiltration and embedding goes.

Congratulations, things are going well!
by mrsonchus
Sun Apr 11, 2021 3:44 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

I don't have any pictures handy right now but the ribbon has a little of that accordion crinkling going on. Going to try to shrink the presentation angle a little bit see if that helps. I rigged up a Sir all ralph knife adapter for the paraffin sectioning kit to hold Feather single edge razors, whi...
by mrsonchus
Sat Apr 10, 2021 8:57 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

I have noticed quite a bit of compression using paraplast with some FAA-preserved trametes. It's a soft wax to be sure, and my cutting room is closer to 75 F where I think this paraplast really prefers to be cut around 65 F. Trametes are pretty woody in my case, though, so maybe I will try some dif...
by mrsonchus
Thu Apr 08, 2021 9:41 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Thanks Jim, dtsh, pippo1234, Pete and mazo4033, that's really nice to know, it's great to be able to share adventures, but I sometimes let my posts get a little dense I think, I have such an interest and enjoyment of Botany and microscopy that I'm inclined to get a little carried away... Thanks for ...
by mrsonchus
Wed Apr 07, 2021 9:09 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Thanks deBult, very nice links. That site is jammed with interesting exploits and no trouble to set the Google translator onto it! This site is where I started with microscopy back in 2015 - feels like home here, and the amount of help and encouragement I've benefited from over those years from the ...
by mrsonchus
Wed Apr 07, 2021 9:01 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Super informative post, John. Thank you! Ideally this will be up for years (decades?) to come and a guide for aspiring microscopists. Thanks PeteM, very kind to say such - much appreciated. It's always great to know folks may have taken a little pleasure from a post, as I do everyday reading throug...
by mrsonchus
Tue Apr 06, 2021 11:25 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Hi Bob, pleased to know you find them interesting - I began to wonder if it was worth the effort of posting to be honest, so little interest outside of equipment and pond-water at the moment. Understandable though as the live-mounts are so accessible. Most folk aren't as time-rich as I am, even with...
by mrsonchus
Sun Apr 04, 2021 11:08 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 839

Re: [HELP] - Hayear Cameras Setup & Software

ToupCams are able to use all functions with the actual ToupView version (i.e. the genuine and original version) whilst others are just using the ToupView with basic webcam capture program functionality. The same funtionality is available with most webcam capture programs such as Windows own camera s...
by mrsonchus
Sun Apr 04, 2021 3:24 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Re: Slide Making with 61°C Wax

Here are some first-look slides from the other day, a selection of different staining and pre-treatments were tried here to see what worked, and of course what I liked.... Some better individual slide-images to follow. 1000px WS_first slide set.jpeg Here's a safranin + alcian-blue stained LS of a mo...
by mrsonchus
Sun Apr 04, 2021 2:19 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 38
Views: 1468

Slide Making with 61°C Wax

Hi all, some will have seen my earlier post re making slides from sectioned Lichen wherein I mentioned that there had been a small amount of tissue-folding during sectioning..... I decided to try a different paraffin embedding wax in an attempt to mitigate and ideally eliminate this phenomenon which...
by mrsonchus
Sun Apr 04, 2021 12:27 am
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 839

Re: [HELP] - Hayear Cameras Setup & Software

The functions are built-into the camera's own software, accessed via the remote control or the buttons on the camera. This menu system will only be usable and visible with HDMI connected screens/monitor. To be controlled from a PC (via USB) the operating sytem of the PC needs the presence of driver-...
by mrsonchus
Sat Apr 03, 2021 7:13 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 839

Re: [HELP] - Hayear Cameras Setup & Software

In your image there is no device shown as recognized in the device list. Are you connected only with USB and is the camera's micro-SD card slot empty?
by mrsonchus
Sat Apr 03, 2021 4:21 pm
Forum: Camera systems and imaging
Topic: [HELP] - Hayear Cameras Setup & Software
Replies: 30
Views: 839

Re: [HELP] - Hayear Cameras Setup & Software

Hi, I too have such a camera, not however a Hayear branded, and unable to use any Toupview re-badge as such. However what I do know is that these cameras, when attached to you PC via USB, have no ability to change the settings of the camera. The settings are only available via the camera's own built...
by mrsonchus
Sat Apr 03, 2021 11:31 am
Forum: Specimens, samples and slides
Topic: My first attempt at using a hand microtome
Replies: 8
Views: 440

Re: My first attempt at using a hand microtome

Very nice start!
A book on plant anatomy would add many layers of interest to your sectioning. The more you look over such revealed details the more the complexity adds to the pleasure and mystery!

Congratulation for these fine sections & images!
by mrsonchus
Sat Mar 27, 2021 11:52 am
Forum: Pictures and Videos
Topic: Aspergillus - Prepared Amscope Slide
Replies: 2
Views: 199

Re: Aspergillus - Prepared Amscope Slide

Must agree, a nice interesting image. Thanks for sharing.
by mrsonchus
Sat Mar 27, 2021 11:20 am
Forum: Identification help
Topic: Any idea what this part of the flower is?
Replies: 11
Views: 326

Re: Any idea what this part of the flower is?

Hi, not even certain it's an orchid - have any parts of the flower's interior been broken away? The pollen is certain though. The protrusions look a lot like 'hairs' as they don't seem to have any elaborated structure to hold 'pollinia' - bundles of pollen as presented by orchids rather than discret...
by mrsonchus
Thu Mar 25, 2021 4:42 pm
Forum: Resources (online, books etc.)
Topic: Cambridge Rocking Microtome Manual
Replies: 3
Views: 3553

Re: Cambridge Rocking Microtome Manual

Have you had a look at a short video I made a while ago of my use of the 'rocker'?
by mrsonchus
Wed Mar 24, 2021 11:17 pm
Forum: My microscope
Topic: 7X-45X Dual Lit 6W LED Trinocular Stereo
Replies: 7
Views: 417

Re: 7X-45X Dual Lit 6W LED Trinocular Stereo

The problem as experienced, that is of the camera's image being only a small part of the total image seen through the eyepieces is a very well-known and common one. Simply-stated perhaps, it's a matter of the image produced at the eyepieces and 'up the photo-tube' being far larger than the size of t...