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What you need to become an amateur fluorescence microscopist, a new class of amateur microscopist. The 21st century kind, a long way since van Leeuwenhoek's 17th century. Then just maybe, some medical doctors with MBBS/MD all over the world will exclaim, "We too also want to become amateur fluoresce...

Old confocal laser scanning microscope pictures.
UV laser light excitation.
Permai fluorescence dye.

Biological identities? Maybe some cilliates or a different kind of paramecium species.

Happy Holidays and Merry Christmas to all!

Greetings all! The Art of Fluorescence Microscopy has moved to a Youtube channel @Art of Fluorescence Microscopy or just do a youtube search on fluorescent artemia/daphnia/moina/ostracod or fluorescence live imaging. Hope you will enjoy the fluorescent videos there! Thank you. :) https://youtu.be/Xo...

Thank you for your kind comments. I am still learning especially on the image processing part.

Always two they are, who walk the dark path of the dark side of microscopy....the "Boss" and her assistant, the "Artist". ;) 1st: Moinas in space, FL 2nd: Daphnia magna, DF+FL or ghost white 3rd: Baby brine shrimp, artistic blue (DF+FL) 4th: Baby brine shrimp, artistic green (DF+FL) 5th: From cancer...

Some new FL pictures from Year 2 Covid-19, the pandemic year continues. Enjoy viewing as usual. Pictures taken from permanent fluorescence microscopy glass slides. ;) :) Pond sub-milimetre creatures as usual. Well....creature biology excites me more than plant biology. :lol: The first one looks like...

An old picture. I used photoshop to improve it. Some kind of oil palm microspores (Malaysia is famous for palm oil :lol: ), small venture of fluorescence into plant biology beside the onion epidermal cells. :) Some new FL pictures from Year 2 Covid-19, the pandemic year continues. Enjoy viewing as u...

Story recapped here. The science of fluorescence microscopy vs the diagnostic of fluorescence microscopy vs the art of fluorescence microscopy. All pictures shown here, have been photoshop enhanced. 1st picture: On left, is the science of fluorescence microscopy with the usual F-actin, microtubules ...

In these difficult and uncertain :roll: pandemic times, I have decided to let go some of my permanent fluorescence microscopy slides to any MH members/hobbyists-amateur microscopists ( rookie or already well-experienced in doing UV fluorescence, spirogyra, acridine orange, etc..) who like to dabble ...

More on the amateur hobbyist-artistic pursuit of fluorescence microscopy. Specific staining is more for scientific endeavour. This is not.

A new year and a new approach to presenting the fluorescence pictures with a little bit of help from adobe photoshop.

Old pictures but manipulated. Enjoy viewing!

The last post. First: Dual moinas. Second: My pond collection has evolved into ostracod culture. Ostracods population just boomed when the water starts to turn murky. Water flea creatures die off leaving only lots and lots of ostracods and copepods. So both ostracods and copepods can co-exist togeth...

Coming to an end.

...........................to be continued............................................

Merged pictures from previous posting,

-----------------------------------------------to be continued..........................................................

First: Cladocera Moina Second: Cyclopoid copepod Third: Ostracod? :roll: dried up, this is from a temporary slide, we viewed this slide last after viewing six permanent slides, after more than 4 hours since we have it prepared. The wet mountant works. Fourth: Same dried up ostracod, higher magnifica...

As the year 2020 draws nearer to end, we have seen strides made by humanity in space explorations such as NASA's OSIRIS-REx, China's Change-5, Japan's Hayabusa 2 space probes despite in the midst of Covid-19 pandemic. So too shall advances been made in amateur microscopy as permanent fluorescence mi...

Last post for the recent photomicrography session. Last 5 pictures. 1st: Some paramecium or ciliates or just pond protists, (I got this right, right? ;) ) BF and FL. 2nd: Starring the biological samples: Kuala Lumpur's pond sub-milimetre scale creatures (my collection..on left), and the infusoria fr...

Here a bit of information about Malaysians or any other South East Asians (the Thais, the Vietnamese, Indonesians, etc..) and amateur microscopy. ;) Believe me, people in South East Asia region are not so keen on this type of hobby. Average Malaysians, if they have money to throw in, in order to ind...

I collected my first Kuala Lumpur pond water samples, my first outdoor biological fieldwork experiences was on this year's June, once the lockdown was lifted. Of course, after going through Oliver Kim's Youtube videos on collecting pond water samples and making some preparations. :) So a little bit ...

1st: Cyclopoida copepod (previous posting), FL merged PH, eye part, 5x 2nd: Mixed cladocera. Daphnia/Moinids/Sidids. BF and FL 3rd: As above, BF merged FL 4th: PH merged FL 5th: Cladoceran ephippia, Sididae family?, BF and FL, the eggs, they all lit up like a christmas tree! :) Enjoy viewing! ;) ......

1st: Small copepodite's body (previous posting)

2nd: Cyclopoida copepod, BF and FL
3rd: Same as above, focusing on its eye, 5x
4th: BF merged FL
5th: BF merged FL, eye, 5x

Enjoy viewing!

to be continued...

1st: Pond microcrustacean a, FL merged PH, 20x

2nd: Cylopoida copepoda (adult) and smaller copepodite, 5x
3rd: BF merged FL, 5x
4th: FL
5th: FL merged PH, 5x

Enjoy viewing!

to be continued...

A journey into biological microscopic heartland in a world that is ravaged by Covid-19. We are exploring pond sub-milimetre scale creatures (include pond protists, pond crustaceans, pond protozoas, pond zooplanktons, etc..excluding diatoms and microalgae) found in Kuala Lumpur, Malaysia. First off, ...

Last post. Last 5 pictures with insets. 1st: Originally 5 slides with 5 samples were intended. We lost one due to carelessness. Amateur do the amateur things :shock: . So 4 made it through the photomicrography session. They are: Copepod, Worms, one small damselfly larvae (refer previous post) and no...

Entering the realm of worms! Yes, worms as in giant monster worm in hollywood movie, Tremors (1990) or that giant sandworm on planet Arrakis. :) Forewarned. I hope the pictures are not too scary for kids. :roll: Stylaria (this time I hope I got this right, :) ), Zeiss A10 inverted fluorescence micro...

Penetrating the pond sub-milimetre scale creatures' domain, sooner or later, after halting at pond milimetre-scale creatures. Remmber, that I am not a biology major, so microorganisms species identification is not my forte, so I will call this, Copepod. Copepod, Zeiss AX10 inverted fluorescence micr...

Damselfly larvae, Zeiss AX10 inverted fluorescence microscope.
BF is brightfield, FL is fluorescence, PH is phase contrast. 5x is magnification.
Enjoy viewing!

Today is a special day here in Malaysia. A fine day for posting :) . First 3 pictures: From E. Coli culture (Leica DM2500 fluorescence microscope). 4th: Tissue section buccal mucosa (Nikon H600L Eclipse 90i fluorescence microscope). Last picture: Yakult Ace probiotic drink microbes (Nikon Eclipse 90...

continuing.... 1st: Mosquito pupa's head. 2nd: "Brown mite", generic name because I do not know what that is :?: :roll:, another milimetre-scale pond creature. Smallest milimetre-scale pond creature here. Maybe we squished it hard :?: and the organelles spilled out ? :?: :o 3rd: "Black Aphid", gener...

Hello All I am afraid I can only do pictures (fulfilled quota of 5 pictures) and not video recording (referring to Introduce yourself- "Hello... from Kuala Lumpur, Malaysia"). Zeiss Axionet Ax10 fluorescence microscope being used here. The first 3 are mosquito larva, and mosquito larva's food ( a mi...