MicrobeHunter.com Microscopy Forum

Close and focus on the Kohler iris. There will be coloured edges. LED is the cause not the objective. There are but the colours vary according to the objective. I can almost get rid of the edges when everything is properly aligned, especially with the Plan Apo and Fluor objectives. 100% what I woul...

My guesstimate is that the three likeliest problems are: 1) Inability to get both good Kohler illumination and a wide enough image circle. One would expect color fringing at the very edges of the illuminated field, but with that mostly outside the field of view with a proper illumination system, th...

How your situation with this objective ended? How was the image? Is this objective spring loaded? I sent this message already via pm, but decided to add it here as well in case someone else is interested: I've been pretty happy with the objective. It seems to perform better than the slightly damage...

Phill Brown wrote: ↑

Mon Jan 29, 2024 7:40 pm

Close and focus on the Kohler iris.
There will be coloured edges.
LED is the cause not the objective.

There are but the colours vary according to the objective. I can almost get rid of the edges when everything is properly aligned, especially with the Plan Apo and Fluor objectives.

Phill Brown wrote: ↑

Mon Jan 29, 2024 10:14 am

If you like yellow and blue fringing LED is an upgrade.

The CFN objectives produce purple and green fringing, and the Fluor objectives some yellow and blue but it's not nearly as bad.

PeteM wrote: ↑

Mon Jan 29, 2024 2:29 am

Was it a DIY or commercial LED retrofit? I've had good luck with Saul's units and also the RetroDiode.

I believe commercial, although it hasn't been installed very well (just seemed sloppy). There's some flickering on low power, but otherwise it appears to work fine as far as I can tell.

Phill Brown wrote: ↑

Sun Jan 28, 2024 10:05 pm

Something is out of alignment.
Wrong bulb or LED "upgrade".
Unlikely to be the objectives.

The scope has been retrofitted with LED by the previous owner.

If anyone has photographs taken through these objectives, I would very much like to see how they look.

Two of your problems may have more to do with your condenser than the objectives. Thanks, Pete. I have two condensers, the Nikon 1.25 phase condenser and Nikon Abbe 1.25. The field of view seems same with both. I'm pretty happy with my other objectives which include a Nikon Plan Apo 20x and a Fluor...

I have a Labophot with the following 4x and 40x objectives, but I want to keep an eye out for better versions. The main purpose is photo and videography. - CFN Plan 40x/0.7 160/0.17 - Fluor 40x/0.85 160/0.11-0.23 - E Plan 40x/0.65 Ph3 DL - E Plan 4x/0.1 160/- - CFN Plan 4x/0.13 160/- The CFN Plan 40...

I feel very very tempted to hop on a ferry to Stockholm (greetings from Finland). Did you use the setup for photography and if yes, do you have any examples? By the way, I can't open all the images behind your link.

The entire optical tube retracts as a safety precaution against both front element and slide damage. I have seen 3 reasons for the optical tube to not retract. 1) The spring has been replaced with a solid spacer, stopping the retraction from taking place. This is a deliberate modification and usual...

Thank you viktor and apochronaut!

Shaver oil. it has a thin nozzle for application to seams and the like. The last time I looked into the Nye Hobby kits, they had been discontinued but a phone call to Nye located 7 at a distributor. I use that oil a lot but as Victor says, other light oils will do, or might do. The problem with col...

viktor j nilsson wrote: ↑

Tue Oct 31, 2023 7:22 am

karhukainen wrote: ↑

Tue Oct 31, 2023 6:39 am

Thanks! Which lubricant/oil do you use?

The light oil from the Nye hobbyist kit.

Do you know if it's been discontinued or perhaps renamed? I couldn't find this kit for sale anywhere, (I checked Micro Tools). There's one kit on Ebay, but who knows what those bottles really contain.

I've fixed the collar on a very similar Nikon CFN Planapo 40x 0.95. You just loosen the screws, which releases the rotating ring. Then you can clean and lubricate the mating surfaces. I don't think they are locked in place, but maybe add a tiny drop of acetone on each screw just in case. Although i...

^ So far, all my iris objectives work just fine (none becomes stuck again after I released them about 3-6 years ago). Only one objective take some effort to turn but still works. The things about tiny iris leaves is that if you mess them up, they are a huge pain to put back. Yeah, that's something ...

It has been a while since I repaired on of those or similarly constructed. The memory banks are full and in need of advice on how to get them unstuck. Just one thing. That JIS screw that sits in the grip ring looks a bit out of place. I might be wrong but is it an original? I wondered about the scr...

I don’t have the exact objective and don’t remember exactly how I released my objective iris or correction collar. I did it successfully with two objectives and have not destroyed any. I think what I did was either applying 2-3 tiny drops of xylene by micro pipette or heating, then turning by force...

Apochronaut mentions Nye PP269 oil in some other thread concerning a stuck iris, but I couldn't find this oil for sale anywhere. It was also mentioned that lubricating a frozen cover glass adjustment collar can be more risky optics-wise and shouldn't necessarily be done without disassembling the obj...

I got this objective from Ebay and it was supposed to be in good working condition, but both the correction collar and the front element are stuck. I was promised a full refund and will get to keep the objective, so now I can try to fix it. Any thoughts on how to start?

Scarodactyl wrote: ↑

Wed Oct 11, 2023 2:08 pm

From what I understand this is a newer production run post-finite era that Nikon did for some OEM usage. Should be great if in good condition.

Can anyone else confirm this?

Item does not match images in the listing. Seller pays return. Full refund. Seller may offer a partial refund. You don't have to accept. Yeah, I know this but would like to know what this objective is. I remember hearing about Chinese cheaper objectives made for Nikon and was thinking maybe this is...

I bought a Nikon 20x Plan Apo on Ebay, and the listing had images of a Nikon CFN 20x Plan Apo, the one with metal coloured barrel and two knurled rings. However, I received another objective instead (experiencing deja vu as apparently this keeps happening to me). I haven't tested the objective yet, ...

How do you clean your jars, nets etc. that you use for collecting and keeping samples from the wild? How do you keep your samples and dispose of ones that are no longer needed? I want to submit observations (zooplankton and phytoplankton and protozoa) on iNaturalist and wish to take care, so that I ...

To add, the usual place the Nikon fluorescence head breaks is toward the back. It's not too hard to put a sleeve on the back end and then put a UV LED at about the same place where the mercury lamp would be. This is a safer and cheaper way to do some UV photography, though you still have to be care...

Is a fully functioning fluorescence scope what you're after? In general, $1500 plus tax & shipping would be the Ebay price for a good BX40 with plan achromats. Plus a bit for the one 40x UPlanFl. You might check Ebay "sold" prices for the going rates. I've got a couple of spare BX40's, and a variet...

What would be a good price for an Olympus BX40 microscope in good condition? The one I'm looking at comes with fluorescence and four (+ possible one but I don't know the specs yet) objectives: - UPlanFI 40x/0.75 - Plan 50x/0.90 Oil Iris - Plan 100x/1.25 Oim - Ach 20x/0.40 /0.17 Everything should be ...

Is your priority microscopy or microscopes? It seems you have a thing for antique/vintage microscopes, but those aren't necessarily that great for microscopy. One essential problem is illumination. Your microscopes seem to have a mirror for this purpose which isn't ideal for achieving even and brigh...

Hilarious stuff! I actually need to create images that resemble the ones (the "nano chip" stuff) in the beginning of this site. What kind of substances might give good results?

I edited the original post a bit because I need buy something quite quickly.