MicrobeHunter.com Microscopy Forum

Remarkable!

LouiseScot wrote: ↑

Fri Oct 20, 2023 9:29 pm

MichaelG. wrote: ↑

Fri Oct 20, 2023 5:35 am

I am sending you a personal message, Louise

MichaelG.

Hi
Can all of this thread be deleted now, please
Thanks
Louise

How to contact global adminisrators/moderators to get this thread deleted?

MichaelG. wrote: ↑

Fri Oct 20, 2023 5:35 am

I am sending you a personal message, Louise

MichaelG.

Hi
Can all of this thread be deleted now, please
Thanks
Louise

apochronaut wrote: ↑

Thu Oct 19, 2023 6:38 pm

He and I have only communicated s little and not for more than a year but he certainly would have reason to be preoccupied with other than mhf. I assume you have sent a private message?

Yes, I did. I was worried about him - I still am. I can only hope he and his family are ok.

Louise

LouiseScot wrote: ↑

Tue Oct 17, 2023 1:43 pm

Hi

I sent a message to the Administrator on the 15th October - received an acknowledgement but didn't get a reply?
I was asking about Hobbyst46 and whether anyone knew of his wellbeing?

Louise

Anyone?

Hi

I sent a message to the Administrator on the 15th October - received an acknowledgement but didn't get a reply?
I was asking about Hobbyst46 and whether anyone knew of his wellbeing?

Louise

Hi Andy Good to see some images of thin sections! I did a geology course at Uni back in 2018 and part of it was looking at various thin sections and learning how to analyse and describe what you were seeing. As it involved microscopy, I naturally enjoyed it! I just ordered some prepared thin section...

I suddenly remembered I have a clone LMPlan 5X inf in place. I'd forgotten! That's what you get when you don't do something for a year especially when you're elderly! I have to remember and relearn everything! Anyway, I took a quick pic of some aluminium kitchen foil: Foil_20pc_DSCF0213.jpg Any opin...

Thanks again, guys! I bought it on an Ebay auction a couple of years ago but I've not done any microscopy stuff for a year. I got it for £290 in the auction - seemed reasonable :) I can see the expected interference pattern through the ocular but haven't been able to properly see a DIC image. But th...

Do I need to convert it back to a finite system for the DIC to work?

Thanks

Louise

They might, but with a Nikon finite metallurgical setup it is awkward to find out because the illuminator just has empty space in it and demands 210mm objectives and 160mm objectives will be pushed way out of spec. There aren't too many non-nikon 210 objectives. That said Nikon also made infinity m...

OK - thanks guys! I'll try and get some in
Um, am I right in thinking non-M Plan wouldn't be expected to work?

Cheers

Louise

Hi everyone I have one of these Nikon turrets with 4 prisms: NikonDICTurret.JPG I was just wondering if anyone on here has experience of using one? If so, do you know if I can use it with a 'standard' Nikon CF M Plan objective or do I absolutely have to have a DIC version? (which I can't find anywhe...

I'm expecting the clip-on lenses today. However, I think they may be somewhat redundant now! I've made adapters for a 10x EP, a 25x EP as well as the lower power plano convex lenses which together are f = 29.5mm. I've just been using the UV torch for illumination. I think I'm sceptical of the paper'...

Great video, as usual, Luis!

Louis

A working fluorescence setup for any microscope could be build from low cost components. A blue LED, a battery and some yellow gelatin filter are all you need. I recommend blue over UV light. It gives much stronger fluorescence from chlorophyll for example and it is saver as well. Picture quality u...

This might be of interest https://www.nature.com/articles/s41598-023-29182-y I'll look into it myself when I get a minute! Louise Hey, wait a minute! Don't you have a DIY DIC system to finish building? I do! I had a road accident on my bicycle when I was 16. Ever since I've had this problem where I...

You can surely do it just for fun and it should work in theory. Though otherwise I don’t see a point of using DIY “microscope” and a smartphone, when you have a real microscope (and objectives) and a real camera (whose noise tolerance is much higher than a phone camera). Image quality in that manus...

Just for fun I ordered a couple of the clip-on macro lenses from Ebay. I'll have a play. Could use them for flowers and insects, if nothing else! For fluorescence, though, I figure I can probably use green and red, maybe cyan, astronomy filters for the emission side. I have blue LEDs for excitation....

This might be of interest

https://www.nature.com/articles/s41598-023-29182-y

I'll look into it myself when I get a minute!

Louise

woyjwjl wrote: ↑

Tue Mar 07, 2023 1:07 am

2023-03-01 11-37-42 (2).jpg

Unless you question Rayleigh's law

What's that in the image?

Do have a go with 100x oil immersion! I'm sure you'd be pleased with the result

Louise

I hope that you do not LOOK into the eyepieces - because the images indicate that direct UV reaches the eyepieces. Hobbyst46 ...thank you so much for spotting this (A piece of white cotton placed over the eyepiece tube gave a startling blue replica of the sample I was looking at ! :oops: ) I will f...

I gave up on astronomy/astrophotography because the skies here are awful, and because I had to do it all from an open window (problematic!), and because I couldn't cope with the window open in cold weather (when it's more likely the skies are clear). It's not necessarily true that you have to buy ex...

dtrinh wrote: ↑

Mon Dec 19, 2022 7:55 pm

Thank you all for very informative inputs. I have ordered one, mostly to have the dark field capability.
Best regards.

Do post a review - and some images, if you can

Louise

Ah the objective is actually mentioned here https://www.microbehunter.com/microscop ... 739#p87739

I would check with the seller that the iris is in good working order...

Louise

Edit: The Nikon CF brochure is a useful resource https://krebsmicro.com/Nikon_CF.pdf

- just means it doesn't matter. A max 0.85 n.a. oil objective will not care one bit about the difference between coverslip glass and oil. But that would depend on the mounting medium? If it was an aqueous sample a cover glass would be needed and the cover glass thickness would matter? I have a feel...

dtrinh wrote: ↑

Sun Dec 18, 2022 12:18 am

LouiseScot wrote: ↑

Sat Dec 17, 2022 11:46 pm

Hi

I think they are no-coverslip objectives

Louise

Hi,
It is written 160/-, so I guess it can be used both with and without coverslip?

Yeah, I was thinking the '-' meant NGC (that would be 0) but I presume there's no value as it's adjusted/determined by the correction collar?

Louise

dtrinh wrote: ↑

Sun Dec 18, 2022 12:18 am

LouiseScot wrote: ↑

Sat Dec 17, 2022 11:46 pm

Hi

I think they are no-coverslip objectives

Louise

Hi,
It is written 160/-, so I guess it can be used both with and without coverslip?

Yeah, I was thinking the '-' meant NGC (that would be 0) but I presume there's no value as it's adjusted/determined by the correction collar?

Louise

Hi

I think they are no-coverslip objectives

Louise