Search found 113 matches
- Tue May 14, 2024 10:42 am
- Forum: Microscopy accessories
- Topic: centering the epi-illuminator
- Replies: 2
- Views: 77
Re: centering the epi-illuminator
On my Zeiss finite units there is an eccentric pin which can be rotated to fine tune the mirror angle. It is locked with a tiny screw. Can you post a picture, there might be something similar?
- Tue May 07, 2024 10:44 am
- Forum: Microscopes and optics
- Topic: Zeiss infinite condensers
- Replies: 3
- Views: 194
Re: Zeiss infinite condensers
Thanks for the response. What you've written matches my understanding. The physical distances on the stands are quite similar, but the optical configuration is obviously quite different. The Universal does have a (removable) diffuser in front of the lamp, but without it the filament is in focus in t...
- Thu May 02, 2024 12:09 pm
- Forum: Microscopes and optics
- Topic: Zeiss infinite condensers
- Replies: 3
- Views: 194
Zeiss infinite condensers
Although I've got a great collection of 160mm Zeiss west microscopes now, I've been curious about the advantages on the infinite system for photography so have been slowly collecting bits. I've now got an Axioplan and 3 Plan Neofluars, but no condenser or holder (ex-fluorescence scope). I had planne...
- Thu Mar 28, 2024 10:17 am
- Forum: Microscopes and optics
- Topic: Field number vs. field stop diameter in "negative" eyepieces
- Replies: 8
- Views: 2766
Re: Field number vs. field stop diameter in "negative" eyepieces
That's my understanding.
Measuring field width via a micrometer slide should determine whether it applies to your particular eyepiece?
Measuring field width via a micrometer slide should determine whether it applies to your particular eyepiece?
- Fri Mar 22, 2024 11:43 am
- Forum: Microscopy accessories
- Topic: Anti-Static for lenses.
- Replies: 26
- Views: 3253
- Thu Mar 21, 2024 12:42 pm
- Forum: Microscopy accessories
- Topic: Anti-Static for lenses.
- Replies: 26
- Views: 3253
Re: Anti-Static for lenses.
My drum scanner came with a large brush labelled as anti-static. I'll look up the make when I get home.
It appears to be made of animal hair. It does seem to work, but the drums are acrylic so no guarantee it'll work on glass.
It appears to be made of animal hair. It does seem to work, but the drums are acrylic so no guarantee it'll work on glass.
- Thu Nov 09, 2023 10:03 am
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55946
Re: Let’s talk about Delamination
Condensation possibly. I don't think it was. I had a good look at the objective and couldn't see any, however there was a patch with the characteristic oily interference colours. There is a good chance to repair de-lamination if you are able to disassemble the item. Yes, I've had a lot of success w...
- Wed Nov 08, 2023 1:52 pm
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55946
Re: Let’s talk about Delamination
10, maybe 15 max.
- Wed Nov 08, 2023 11:35 am
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55946
Re: Let’s talk about Delamination
Just had an interesting delamination experience. I bought a 10x Zeiss Planapo as an upgrade to my 10x Neofluar. The box was on my step when I got home from work on Friday. I unpacked it and attached it to my Universal. Immediately I saw a problem - I couldn't get a sharp image. The overall image was...
- Tue Jul 11, 2023 1:11 pm
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
Problem with the dyes is the terrible mess if the dye solution (or powder) is accidentally spilled Thanks for the warning! Also note, that fluorescence brightness benefits from high NA, since the amount of light gathered by the objective strongly grows with the NA. Yes, I was aware of this and took...
- Tue Jul 11, 2023 11:42 am
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
Thanks again for the advice. Actually had some success last night after going through my box of prepared slides - a couple of stem sections showed a fairly strong result in Position 4. The top one is a sunflower stem. There's no info on stains used. AL-7159.jpg AL-7162.jpg The illumination reaching ...
- Fri Jul 07, 2023 9:30 am
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
Thanks both, useful info. There are no markings on the filter rims. Here are some pictures with afternoon sunshine as the illuminator: Exciters, hopefully showing both reflection and transmission: IMG_6082.jpg Slot I is empty, slot 2 is dye filters only so only 3 and 4 the reflections from the inter...
- Wed Jul 05, 2023 1:24 pm
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
Thanks. The III RS doesn't have cubes, it has two sliders. One holds the exciter filters, these are push fit and can be easily changed, the other slider has the mirrors and emission filters held more securely. So in use it's possible to experiment by swapping the exciter set with the mirror/barrier ...
- Wed Jul 05, 2023 11:30 am
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
Yes. Fluorescence in general is dimmer by orders of magnitude than the excitation light. Often, fluorescence microscopy is done in a half-darkened room. Hmm. had a 'look' at some moss last night. With my 30W halogen lamp at full power (same luminance as the more common finned tube 60w lamphouse), i...
- Tue Jul 04, 2023 10:23 am
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Re: Zeiss III RS - Getting started in Fluorescence
The commonest and easiest fluorophore is chlorophyll, either in green plants or in briophytes or algae. I would suggest the latter. Moss "leaves" are excellent. The best excitation is violet light of wavelength 405-425nm, NOT UV. A peace of moss in water will fluoresce red. Thanks, I'll give that a...
- Mon Jul 03, 2023 12:38 pm
- Forum: Illumination Techniques
- Topic: Zeiss III RS - Getting started in Fluorescence
- Replies: 19
- Views: 4561
Zeiss III RS - Getting started in Fluorescence
I recently picked up a box of Zeiss bits which included a Zeiss III RS head which I've been playing around with on my Universal III. The filters are mostly in good condition thankfully. One beam splitter has a bubbly look but still gives a viewable image. A few of the dye filters had hazy surfaces w...
- Mon Jul 03, 2023 12:09 pm
- Forum: Microscopes and optics
- Topic: Zeiss finite objectives 20x to 32x?
- Replies: 7
- Views: 1393
Re: Zeiss finite objectives 20x to 32x?
. . . Eyepieces and telan lenses are a different matter, at least half are seriously delaminated, but thankfully repair is easier. . . Adam - - curious -- have you found a way to repair delamination in the intermediate pieces (finite adjusting telan lenses)? I've heard of some folks soaking them in...
- Wed Apr 19, 2023 12:33 pm
- Forum: Microscopes and optics
- Topic: How many microscopes are enough? :<)
- Replies: 31
- Views: 9410
Re: How many microscopes are enough? :<)
I accumulated four Zeiss standards before making room for a Universal. It has a big footprint but is now truly worthy of the name with all the lighting options I want plus easy switching between, as well an Optovar and Trino head built-in. Stage and condenser are easily adjusted or removed as requir...
- Wed Apr 19, 2023 12:13 pm
- Forum: Microscopes and optics
- Topic: Zeiss finite objectives 20x to 32x?
- Replies: 7
- Views: 1393
Re: Zeiss finite objectives 20x to 32x?
1) I must have about 30 Zeiss objectives now. Only two show any delamination, only one of which, 16x PH2 Neofluar, seriously degrades the image (fine detail is doubled). The other, Planapo 25x, shows multiple planes of delamination but limited to the periphery so still gives good images. Eyepieces a...
- Wed Mar 29, 2023 12:17 pm
- Forum: Microscopes and optics
- Topic: Recommendations for 40x-100x Objective, LWD, High n.a.?
- Replies: 29
- Views: 4197
Re: Recommendations for 40x-100x Objective, LWD, High n.a.?
I have a Zeiss LD-Epiplan 40x/0.60 which might worth a look. They are not common on the used market but not sought after; mine was £50.
I will have to check the working distance but from memory it is a few mm.
I will have to check the working distance but from memory it is a few mm.
- Tue Jan 17, 2023 1:46 pm
- Forum: Microscopes and optics
- Topic: Zeiss 4X objective
- Replies: 20
- Views: 4055
Re: Zeiss 4X objective
I have the Zeiss 2.5x (modern version), the 6.3x Neofluar and the 4x Planapo (older version). The 6.3x NF has by far the most working distance, and unlike the 2.5x the objective is tapered which gives even more room. The older 2.5x looks a little narrower than mine, but not as much as the 6.3x. I wo...
- Tue Jan 03, 2023 2:43 pm
- Forum: Microscopes and optics
- Topic: Information on the "Old" Zeiss DIC System for Finite Microscopes
- Replies: 2
- Views: 708
Re: Information on the "Old" Zeiss DIC System for Finite Microscopes
Thanks Tim, very useful. I got an old Zeiss DIC set in the autumn and am very happy with it. With tweaking I've found it gives good results with most of my objectives although backgrounds are typically uneven. My favourite combinations are the 16x Neofluar with Prism I and the 63x Neofluar 1.25 oil ...
- Thu Sep 29, 2022 12:33 pm
- Forum: Pictures and Videos
- Topic: Microscopic life in a sea water aquarium
- Replies: 21
- Views: 4859
Re: Microscopic life in a sea water aquarium
I fine lens indeed, what sort of trans DIC combination have you come up with? Just this week I received a condenser and slider of the first generation. The parts are a little beaten up but the results are great so far, despite not using the correct objectives. Previously I did play around a little ...
- Wed Sep 28, 2022 9:05 am
- Forum: Pictures and Videos
- Topic: Microscopic life in a sea water aquarium
- Replies: 21
- Views: 4859
Re: Microscopic life in a sea water aquarium
Fantastic images Wes!
The Planapo 25/0.65 is also one of my favourite objectives. It seems to play nicely with the first generation of Zeiss DIC too, though it is early days for me with trans DIC.
The Planapo 25/0.65 is also one of my favourite objectives. It seems to play nicely with the first generation of Zeiss DIC too, though it is early days for me with trans DIC.
- Mon Aug 01, 2022 10:03 am
- Forum: Pictures and Videos
- Topic: Amphora optical sections
- Replies: 11
- Views: 2377
Re: Amphora optical sections
Hi Adam, Thank you for your comment. The diatom measures at 40x60 µm. I used DIC with a 40/0,95 apochromat Zeiss Jena objective, the microscope was setup for afocal projection with a 10/18 Kpl-W eyepiece (with a 40 mm pancake lens on a Canon 50D). The diatom was attached to the underside of the cov...
- Fri Jul 29, 2022 12:19 pm
- Forum: Pictures and Videos
- Topic: Amphora optical sections
- Replies: 11
- Views: 2377
Re: Amphora optical sections
Great results Wes.
I've recently been experimenting with pushing the resolution limits of my Zeiss Universal on similar subjects, so any technical details would be appreciated. And how big is this diatom?
I've recently been experimenting with pushing the resolution limits of my Zeiss Universal on similar subjects, so any technical details would be appreciated. And how big is this diatom?
- Wed Jul 20, 2022 9:23 am
- Forum: For forum members who want to buy and sell equipment
- Topic: looking for an Zeiss KPL 10X eyepiece
- Replies: 3
- Views: 1161
Re: looking for an Zeiss KPL 10X eyepiece
Me too. I'm in the UK.
- Wed Jul 20, 2022 8:25 am
- Forum: Beginner's corner
- Topic: Looking at opaque objects with compound microscope
- Replies: 6
- Views: 2163
Re: Looking at opaque objects with compound microscope
You can, but you need epi-illumination which means the light comes from the top through the same objective used for viewing. Yes, the focal plane and working distances become very limited, but in my experience it can be more user friendly at higher mags than working with water-on-slide mounts and tr...
- Thu Jul 14, 2022 9:55 am
- Forum: Microscopes and optics
- Topic: Zeiss achroplan stuck
- Replies: 21
- Views: 3245
Re: Zeiss achroplan stuck
The only good resource I've found is here: https://www-funsci-it.translate.goog/schede-tecniche.html?_x_tr_sl=it&_x_tr_tl=en&_x_tr_hl=en-US&_x_tr_pto=wapp I can't see any Zeiss infinity optics unfortunately. There was also this recent thread: https://www.microbehunter.com/microscopy-forum/viewtopic....
- Thu Jul 07, 2022 2:09 pm
- Forum: Home-made microscope adaptations
- Topic: DIY epi-illumination through eyepiece port
- Replies: 4
- Views: 2505
Re: DIY epi-illumination through eyepiece port
To be honest this can be a problem even with dedicated epi heads (I have a Zeiss Universal). Crossed polarisers are the solution, albeit with the loss of much light, but closing down the field diaphragm to exactly match or slightly vignette also helps a lot. So I'd suggest looking at ways to collima...