MicrobeHunter.com Microscopy Forum

I ended up going with a xenon bulb from a car How well does it work e.g. with a GFP/FITC filter? How is the intensity? With the filter Im using I believe 430-490nm the intensity is plenty to be picked up by a cell phone camera , (see video) I have not used any dyes, just Autofluorescence for now, I...

[] I bought a universal power supply from Ebay. Actually I have been stuck with the fluorescence method itself in the lab. Thanks for reminding me. I try to start this project soon. Too many projects... :) I ended up going with a xenon bulb from a car https://www.microbehunter.com/microscopy-forum/...

Just a handful of bacteria can be identified by sight and even that requires complex stains like acid fast, endospores malachite green etc... The image you uploaded appears to show septate or multinucleated hyphae from a fungi and not a bacterial colony., but even then Im not 100% sure. You can try ...

Im having a hard time distinguishing between the two of them... probably due to a certain degree of color blindness
Would you call this positive or negative? https://youtu.be/SG_tT_oZDBc

https://youtube.com/shorts/y7HFcdajvmk?feature=share

Perfect my setup is missing the 100X, this site is offering them in positive and negative, Im not sure if negative offers any benefits over positive at such higher magnifications. any advantages of one over the other? Thank you. https://bolioptics.com/100x-infinity-corrected-plan-ph-n-achromatic-neg...

Can a negative phase objective be used with a positive phase condenser and vice versa?

Didymium filters are very useful indeed - I have a number of Olympus filters that I use/d with my BX40 and 50 'scopes. Hi John, I'm looking to use purchase and use for prepared histology slides and on my Fl Laborlux, particularly with eosin; wherein the light path do you normally use yours? does it...

Are all the parts interchangeable in between the Reichert 400 and 420 Microstar/Diastar? Particularly the nose turret being that the Diastar has a slot for a filter. Also the Diastar having the 100w lamp housing Im guessing was used primarily for high mag DF and some form of DIC, but whats inside th...

Didymium Filters has anyone used them or are familiar with them, also are Didymium Filters the same as intensifier filters used in photography like the hoya ones or is it a completely different material?
https://www.olympus-lifescience.com/en/ ... /didymium/

The ones you are looking for have Chrome barrels with either engraved ( earlier) or painted ( later) Reichert on the barrel. They are a wider barrelled objective than the catalogued series 400 objectives. Im still getting confused when it comes to the 26mm from thePolyvar and the chrome RMs apo/Fl ...

One major advantage of led light is that you can switch it on and off as often as you need. Conventional fluorescent lamps do not like turning on and off frequently. There are several discussions on this forum about this topic if you search. I have also built a fluorescent led light, however using ...

Is that a tube lens? I had overlooked some images early on and assumed the tests were done with the Microstar IV(MS4) head and swapped eyepieces, which prompted my question about the telan/tube lens. If, however, the tests were done with the Laborlux head (is that a Laborlux head?), Microlux head, ...

microb wrote: ↑

Wed Nov 03, 2021 6:32 pm

Is that a tube lens?

I believe so, not 100 percent sure, there is a marking in front of the Illuminator " 1X"

It looks like you could do an adapter that is basically a tube. I didn't model the top inset anchor-points/bearing-pads. And in this image showing a cross section, it would be way shorter. Also the angle of the dovetail is just arbitrarily 45 degrees, but Olympus is 60. So that is probably wrong. B...

. "F.Y.I. If this is for your Frankenstar, I would keep my eyes peeled and hold out for Reichert planapos, unless you are in a rush." Indeed, I plan on using them on the Frankenstar, I have not run into one yet, and honestly don't even know how they look like, are they stamped AO or Reichert? I wou...

Im thinking of pulling the trigger on some of these "Chilympus" objectives, and was wondering if its the right way to go. Overall I'm jus trying to get a better image out of my systems, I've seen some apos on ebay but even used they are a lot more expensive. Any input is appreciated

Like any other halogen bulb it produces an quite significant amount of IR. This is why those lamp houses came with heat filters. If you have one of the Illuminator 100 lamphouses can you point out the heat filter for me. Only glass between the bulb and the microscope that I can see is the focusing ...

More pics

] So the LaborLux, does it use 210mm objectives with reflectance? Im not sure, I know that the Laborlux 12 can use 160/170mm objectives What objectives did you use on the MicroStar? Are they infinity, or did you just test phase contrast with infinity and use a trinocular with a tube lens? The Micro...

I presume that's using the telan from the Microstar? Not sure what telan is?!! As for the illuminator, I've done some 3D modelling and have a Microstar IV if that might be of assistance. The vertical illuminator that I plan on adapting is from the Laborlux (160/170mm) the clamp mechanism as well as...

I can't see it in your images, but is there not enough travel for the spring loaded condenser stop to the Microstar stage? If it matters, I suspect one could modify that part relatively easily. . Yep comes off short by 1 mm when the condenser is raised all the way up in case I want to use Immersion...

Here's a video testing DF and phase
https://youtu.be/EkekYVEtNC8

microb wrote: ↑

Mon Nov 01, 2021 5:52 pm

I'd be curious what dovetail designs you need to match. If you look at my posts, I've been doing some adapters.

Here's a picture of the Microlux dovetail where the phase kit came from, with an abbe condenser from the manufacturer.

My mistake :? , there is a way of installing the condenser without removing the stage,... The stage adjustment can be moved all the way up, with nosepiece completely raised and that gives you enough clearance to remove the condenser without having to remove the stage. ... You will have to be mindful...

Notice where the pins grab onto the condenser, it comes up to exactly 45mm on the Chinese condenser.

I'd be curious what dovetail designs you need to match. If you look at my posts, I've been doing some adapters. Ok so I took ot apart to check the measurements, only off by a few decimal points when you compare the two, I measured a AO1087 condenser as well as the 1970 and there is a 2-3 mm differe...

I have really enjoyed reading about these experiments with car xenon lights. Do you have a link describing those experiments? Sounds intriguing. It started with a thread on the Amateur Microscopy page on Facebook, but I couldn't find it again when I look for it. Edit: I found it, it is in the discu...

Heres a quick video at 600X ( 15X eyepiece) using a cellphone.
https://youtube.com/shorts/ttC6q-tLogQ?feature=share

Here's a Microstar IV with Phase contrast from a "Olympus"copy condenser and matching objectives (Infinity). The phase kit came from the Seiler Microlux IV you see on the background, also goes by BK5000 sold by Jenco or straight from China. Installation requires removal of the stage on the Microstar...