Search found 4057 matches

by apochronaut
Tue Apr 20, 2021 7:48 pm
Forum: Microscopes and optics
Topic: Any Bresser XPD-101 field microscope users?
Replies: 6
Views: 67

Re: Any Bresser XPD-101 field microscope users?

I was told by a rep. from a Chinese factory recently that the Chinese made FM-31 clone is no longer being made so if Bresser has them still, they may be the last of them.
by apochronaut
Tue Apr 20, 2021 7:22 pm
Forum: Beginner's corner
Topic: Knurled ring on 60X objective
Replies: 6
Views: 65

Re: Knurled ring on 60X objective

A picture would help but it is most likely one of two things. 1) a correcting collar which moves internal lens elements to correct for spherical aberration caused by using a coverslip other than one of ideal thickness. 2) an iris diaphragm control that is used to reduce the N.A. of the objective, wh...
by apochronaut
Tue Apr 20, 2021 5:17 pm
Forum: Microscopes and optics
Topic: Most useful magnifications for phase contrast
Replies: 8
Views: 128

Re: Most useful magnifications for phase contrast

AO/Reichert made a phase condenser with a single insert that could be slid in or out for phase contrast. They apparently thought a 40x was most useful, since this was the default configuration. For many really interested in cell details, it's probably a good decision. Working with kids, I find a 20...
by apochronaut
Tue Apr 20, 2021 12:06 pm
Forum: Microscopes and optics
Topic: Most useful magnifications for phase contrast
Replies: 8
Views: 128

Re: Most useful magnifications for phase contrast

If I think of my usage over the past years, I would say the 20X has been the most used but they all get used a lot. I don't use phase objectives for BF. They just aren't good enough. Instead, I sought out objectives of higher N.A. and better colour correction. They can produce imaging that rivals ph...
by apochronaut
Tue Apr 20, 2021 1:56 am
Forum: Microscopes and optics
Topic: Can't focus new 60X objective
Replies: 17
Views: 194

Re: Can't focus new 60X objective

One of my big problems with the objective was how far out of center it was ; 200 microns or so. The high magnification Reichert objectives are rarely out by 20. Nevertheless for 80 bucks it was o.k. I guess but I just spent 125.00 for a 40X 1.0 oil planapo. If I put 15X eyepieces on that, it makes t...
by apochronaut
Mon Apr 19, 2021 10:49 pm
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 5
Views: 204

Re: should i buy Bresser phase contrast series 57?

The Galen III was an earlyish Chinese D.I.N. scope. The objectives show up as Bausch & Lomb, Cambridge , Leica and generic planachros from China. The phase ones I have seen have all been black. This is probably a new set on right now, badged Leica. The seller doesn't list items out of their packagin...
by apochronaut
Mon Apr 19, 2021 10:35 pm
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 13
Views: 258

Re: AO 1240 phase condenser (for model 10) on 110

Yes, my mistake. E200 it should have been.
by apochronaut
Mon Apr 19, 2021 10:10 pm
Forum: Microscopes and optics
Topic: Is Slide thickness critical?
Replies: 5
Views: 92

Re: Is Slide thickness critical?

Currently, a slide thickness of 1mm is considered optima for most applicationsl. In the past, thicker slides were more often used, ahout 1.2mm. The thickness of the slide does not affect the performance of the objective lens directly but does indirectly by affecting the performance of the condenser....
by apochronaut
Mon Apr 19, 2021 9:33 pm
Forum: Microscopes and optics
Topic: Can't focus new 60X objective
Replies: 17
Views: 194

Re: Can't focus new 60X objective

Yes. Your objectives are infinity. That's why that one doesn't work. Don't waste your money on parfocalizing shims. The image will be terrible when you do get it in focus.
by apochronaut
Mon Apr 19, 2021 9:05 pm
Forum: Microscopes and optics
Topic: Can't focus new 60X objective
Replies: 17
Views: 194

Re: Can't focus new 60X objective

That objective is for a Chinese short barrel microscope with a 160mm tube length. They made them up until the 80's as lab grade microscopes but still offer them for sale, since some of the old designs are still made for student microscopes. They have a lot of kids to teach. There has been so much in...
by apochronaut
Mon Apr 19, 2021 1:08 pm
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 5
Views: 204

Re: should i buy Bresser phase contrast series 57?

It is a good idea you asked that question. My answer would be , don't. Simply because the package mentions nothing about the objectives being plan. They appear to be achromats only which sets them back in time to the early days of phase contrast and thus they will be no better, and likely worse due ...
by apochronaut
Mon Apr 19, 2021 12:03 pm
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 13
Views: 258

Re: AO 1240 phase condenser (for model 10) on 110

I would set up one of the series 10 microscopes for phase, if I were in your shoes. Spend the time to clean and properly adjust one. A push pull type cork, the kind with the plastic knob glued on will work for the trinocular cover or pick up one of the photo tubes on line. The knock off ones may sti...
by apochronaut
Sun Apr 18, 2021 4:49 pm
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 13
Views: 258

Re: AO 1240 phase condenser (for model 10) on 110

occasional 1240 condensers will slide into a series 100 yoke and locate by friction rather than by registration, since both the condenser and yoke will have registration grooves, rather than pins. the fit is tight and will work o.k. you can encourage a fit with a file. however, fit is only one of th...
by apochronaut
Fri Apr 16, 2021 12:50 pm
Forum: Illumination Techniques
Topic: Researchers create light waves that can penetrate even opaque materials
Replies: 3
Views: 123

Re: Researchers create light waves that can penetrate even opaque materials

When does the first transmitted illumination met microscope come out?
by apochronaut
Thu Apr 15, 2021 11:55 am
Forum: Microscopes and optics
Topic: Nikon Plan objective - washed out colours
Replies: 12
Views: 292

Re: Nikon Plan objective - washed out colours

No achromat has full spectrum colour correction. In fact, no objective has full spectrum colour correction. If achromats could do it, then fluorite and apochromat objectives wouldn't exist and even they are on a never ending treadmill leading to perfection somewhere near to infinity. Companies will ...
by apochronaut
Tue Apr 13, 2021 10:41 am
Forum: Microscopes and optics
Topic: Nikon Plan objective - washed out colours
Replies: 12
Views: 292

Re: Nikon Plan objective - washed out colours

All microscope objectives used properly in a coherent system made during any era of manufacturing by reputable companies have fairly equivalent performance. There are differences between them but it is quite subtle. One manufacturer might show slightly lower saturation of red for instance but have h...
by apochronaut
Tue Apr 13, 2021 1:35 am
Forum: Identification help
Topic: Mold, bacteria biofilm, parasite, or something else?
Replies: 7
Views: 4773

Re: Mold, bacteria biofilm, parasite, or something else?

I have to agree more than in part with you. Forum rules are necessary ; they aren't the problem ; what is, is the banal interpretation of them by some ; that any kind of request for i.d. on anything related to the human body is medical advice. This is a microscope forum. All kinds of samples can be ...
by apochronaut
Sun Apr 11, 2021 8:15 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 567

Re: can Phase contrast kit work WITHOUT Köhler ???

I should have added that the condenser is never oiled for phase. It works at sub 1.0 N.A. but arrives at that differently than DF.
by apochronaut
Sun Apr 11, 2021 4:09 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 567

Re: can Phase contrast kit work WITHOUT Köhler ???

There are more types of illumination than just critical or Köhler. Those are just two extremes related to how focused or defocused the illumination source is. Critical illumination requires that the illumination source be very broad and quite uniform in illumination intensity. It was the illuminatio...
by apochronaut
Fri Apr 09, 2021 10:51 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 1569

Re: PZO DIC on a BHT microscope, possible?

Uhhhhh! I am thinking. Why not just buy a Biolar microscope body? You can probably even get the nice 100 watt version with a 5 nosepiece.
by apochronaut
Thu Apr 08, 2021 2:33 pm
Forum: Beginner's corner
Topic: what is a 1x objective ?
Replies: 5
Views: 494

Re: what is a 1x objective ?

Corrected wide field.
by apochronaut
Thu Apr 08, 2021 2:31 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 687

Re: Glycerol Immersion Objectives

Presumably your monochromatic condition is close to one of the wavelengths that the objective is corrected for?
by apochronaut
Thu Apr 08, 2021 1:49 pm
Forum: Illumination Techniques
Topic: DIY phase anulus with LOMO phase objectiv
Replies: 12
Views: 650

Re: DIY phase anulus with LOMO phase objectiv

Agree with Fan. The image of your phase diaphragm should be conjugate with the phase annulus in the objective , when the condenser is focused perfectly on the sample. Where you are placing the phase diaphragms is more typical of the ideal location of a DF occlusion. Typically, phase condensers are o...
by apochronaut
Thu Apr 08, 2021 12:37 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 687

Re: Glycerol Immersion Objectives

As with so many things , there is theory and then there is empirici. There does seem to be a bit of a phobia that plagues newly hatched microscopists when it comes to oil immersion. It is a little difficult to master but immersion is immersion and outside of an extra minute or two to clean up a bit ...
by apochronaut
Wed Apr 07, 2021 12:52 am
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 687

Re: Glycerol Immersion Objectives

Quartz cover slips might be of some advantage but the conventional suppliers want a pretty penny for them. I just happen to have some tweaked water immersion objectives coming from China soon and they are sending some quartz covers and slides at the same time. I am not sure about the price but it wi...
by apochronaut
Tue Apr 06, 2021 8:04 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 687

Re: Glycerol Immersion Objectives

I sometimes use a 63X 1.0 N.A. glycerin immersion planachro. It is very good but since I don't also have the compatible 100X 1.25 glycerin immersion planachro, it is a bit cumbersome. There are very few objectives that can be used with multiple immersion media, without having adjustment collars. Thi...
by apochronaut
Mon Apr 05, 2021 7:45 pm
Forum: Beginner's corner
Topic: Can someone help me understand this?
Replies: 8
Views: 407

Re: Can someone help me understand this?

They will work fine as a set. It is possible though, that in order for them to perform to perfectly planar well corrected specs. they would need eyepieces other than what you have on your microscope. Amscope buys parts and entire systems from multiple factories and jobbers, so full optical compatabi...
by apochronaut
Fri Apr 02, 2021 1:37 am
Forum: Identification help
Topic: Fungus on Pear?
Replies: 2
Views: 175

Re: Fungus on Pear?

Spores are very tiny. Just a few microns across. You may be seeing some fruiting bodies but not spores. If the fungus was black, it might be what is commonly called rhizopus rot by pomologists.
by apochronaut
Fri Apr 02, 2021 1:32 am
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 22
Views: 839

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

From the size of the oil globule or what looks like one, it seems the oil might be high in the lens column. Usually it is a smaller less noticeable contamination when it is down near the front lens. Sometimes it is hard yo see at all.
by apochronaut
Thu Apr 01, 2021 5:54 pm
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 22
Views: 839

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

There are several scenarios with such a lens. 1) the oil has invaded minimally and it simply requires cleaning of the oil , out of the lens chamber and major reduction of the oil in the correction collar and retraction cylinder. That is a fairly easy scenario and is the most likely with a modern len...