Search found 4200 matches

by apochronaut
Fri Jun 11, 2021 12:10 pm
Forum: Microscopes and optics
Topic: Wattage on light source
Replies: 5
Views: 135

Re: Wattage on light source

The location of the illuminator is of important in determining the brightness of an illumination system. Lamps located right under the stage of low wattage, can be brighter than one located in the body or rear of the microscope even though they are of higher wattage. However, if the illuminators are...
by apochronaut
Fri Jun 11, 2021 11:53 am
Forum: Beginner's corner
Topic: which is the real DIN 160mm objective?
Replies: 10
Views: 232

Re: which is the real DIN 160mm objective?

In order to determine an objective's parfocality, you need a high magnification example of the objective series. Low magnification and l.w.d. examples are often much shorter than the parfocal length. An immersion example will be less than 1mm shorter than the parfocal length. In general there are a ...
by apochronaut
Thu Jun 10, 2021 4:40 pm
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

of course. I was more talking about the possibilities for average microscopists with limited budgets. Neatest thing I have seen so far is the concept , which has a prototype working at some level, of pre-magnification with nano lenses. They are claiming 25,000 diameters. Any image reconstruction sys...
by apochronaut
Thu Jun 10, 2021 2:02 pm
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

That's true but light microscopy still has severe limitations in viewing small structures but increasing capability in viewing process.

We still haven't broken the .2 micron barrier although there are rumours of various advances. Pre-magnifying with nano lenses, lots of digital modifications.
by apochronaut
Thu Jun 10, 2021 1:29 pm
Forum: Beginner's corner
Topic: Amscope brand
Replies: 7
Views: 275

Re: Amscope brand

I would be a fruitless chat. I had one fairly recently with a manager at Olympus. Olympus, manufactures components in whichever country is best suited to the task, is pretty much the drill. With Amscope the situation is the same but in their case, they don't manufacture anything. Ningbo is just one ...
by apochronaut
Thu Jun 10, 2021 11:14 am
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

In no specific order. Henrici's Molds, Yeasts , Actinomyces 1930 Pathogenic Micro-Organisms including Bacteria and Protozoa William Hallock Park Anna W. Williams 1910 A Textbook of Bacteriology Hans Zinsser Frederick F. Russel 1922 Bergey's Manual of Determinative Bacteriology 1923 Bacteriology Rich...
by apochronaut
Thu Jun 10, 2021 10:22 am
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 294

Re: trinocular head

Well. I am comitted to the 88.00. . We will see. It is the 100/0-0/100 split I use most on the Diastar, rarely the 20/80. This head from India is claiming extra wide prisms in order to handle an extra wide f.o.v., whatever that means. To some, 20mm might be extra wide. I would see it as 22mm with R....
by apochronaut
Thu Jun 10, 2021 3:20 am
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 26
Views: 1430

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

The threads are kind of lousy but I got it fixed and working well. I don't think that set ring will come out too easily again but it shouldn't need to.
by apochronaut
Thu Jun 10, 2021 2:38 am
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 294

Re: trinocular head

That is very useful. I have my German English fictionary at hand. The 100-0/30-70/0-100 head sounds like the one. I have a similar head for the Diastar only 20-80 and I love it. Yes. I don't know the tube distance. I will have to see. At least the Indian head is new and won't need to be cleaned???
by apochronaut
Thu Jun 10, 2021 2:26 am
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

Even in the 19th century, bacteriologists were using BF to understand their chosen passion with a surprising degree of accuracy.They applied oblique illumination and with patience they observed behaviour and noted it. Early research microscopes were always equipped with sophisticated oblique condens...
by apochronaut
Wed Jun 09, 2021 6:46 pm
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 294

Re: trinocular head

Thanks, Bob. Yes, those are available but by the time I get it here, especially if customs steps on it, it will be possibly 350 $CDN. I'm building up a 100 watt Biolar piece by piece. Two interchangeable 5 place nosepieces; one with a 5X scanning objectve and then the 4 PZO planachros with the integ...
by apochronaut
Wed Jun 09, 2021 11:39 am
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 294

Re: trinocular head

The Indian microscope industry began using tooling and obsolete designs from Zeiss, if my memory serves me correctly. They are probably still using some of the same castings. A look at the microscopes section of Indiamart will give you an idea of where they are at. They still pump out tens of thousa...
by apochronaut
Wed Jun 09, 2021 10:43 am
Forum: Miscellaneous
Topic: 24,000 years on ice
Replies: 2
Views: 115

Re: 24,000 years on ice

Over the years I have repeatedly rehydrated dried up slides, some of which sat for a long time. Any encysted rotifers popped up in no time. One had been down behind the leg of a table and the wall for 1/2 a century I think. Maybe a little less. The rotifers were wearing paisley shirts, though. The p...
by apochronaut
Wed Jun 09, 2021 2:25 am
Forum: For forum members who want to buy and sell equipment
Topic: American Optical Series 10 - $125.00 (works, cosmetic flaws)
Replies: 4
Views: 168

Re: American Optical Series 10 - $125.00 (works, cosmetic flaws)

Note that the field stop shows a yellow diffraction band, the hallmark of fine compensating optics. Don't let the advertising of the pushy companies fool you. ALL microscope optical systems are compensated. Some claim in the objective, some claim elsewhere. It doesn't actually matter where, as long ...
by apochronaut
Wed Jun 09, 2021 12:22 am
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 294

trinocular head

I am still wandering in the vacant landscape of PZO trinoculars. I have looked at the possibility of a 42mm Olympus retrofitting onto a PZO but even there, I am likely going to get dinged for a lot for something that will need work in order to work. Then I find this. https://www.ebay.ca/itm/15427088...
by apochronaut
Tue Jun 08, 2021 9:20 pm
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

Masks are types of filters that reroute, reshape, reduce or block portions of the illumination beam. They can be used in combination with one another or singly. A type of relief contrast, somewhat like DIC for instance can be achieved using masks. A slit condenser, which is a form of malleable mask ...
by apochronaut
Tue Jun 08, 2021 6:52 pm
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 332

Re: Viewing Bacteria With Bright-field Microscopy

Bacteria can be difficult because many of them are quite transparent, however there are several techniques that you can use in BF. To get a practice culture, take some vegetable : cabbage, cauliflower, broccoli, turnip, radish, carrot, cucumber, zucchini, beet ; something firm and with a skin or rin...
by apochronaut
Tue Jun 08, 2021 6:01 pm
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 26
Views: 1430

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

I finally set aside a bit of time last night to tackle this. There are 7 combined cells.The front one will stay and there is one stubborn meniscus lens still in there. The oil causes a vaccuum, so quick removal is not possible. However due to the oil, it will slowly slide down the barrel with gravit...
by apochronaut
Tue Jun 08, 2021 10:41 am
Forum: Home-made microscope adaptations
Topic: AO Cycloptic Illuminator
Replies: 7
Views: 496

Re: AO Cycloptic Illuminator

For anyone interested there are quadrant selectable off the shelf units with an over 70mm i.d. rated at 6000K, as well as smaller ones. They can be bought for around 80.00. The nice thing about them is that like the illuminators made for the higher end modern stereos, they are broken up into quadran...
by apochronaut
Tue Jun 08, 2021 12:24 am
Forum: Microscopes and optics
Topic: Lomo specs.
Replies: 0
Views: 52

Lomo specs.

I am contemplating buying some Lomo D.I.N. planapo objectives. They appear to have made both 160mm and infinity versions. I am more interested in the 160mm right now. Does anyone have any experience with either of those formats? I assume the 160mm need plancompensating eyepieces.Do only Lomo work we...
by apochronaut
Mon Jun 07, 2021 12:44 pm
Forum: Beginner's corner
Topic: Random Questions about objectives and sample preparation
Replies: 3
Views: 151

Re: Random Questions about objectives and sample preparation

The BF objective that you linked to has a specific cover glass spec. of 1.1, since it is for an inverted microscope. You should choose one for an upright microscope that either is a broader cover medium range or one that has the same cover slip correction as the other objectives. It should be marked...
by apochronaut
Mon Jun 07, 2021 10:03 am
Forum: Beginner's corner
Topic: Amateur microscopist discoveries?
Replies: 8
Views: 386

Re: Amateur microscopist discoveries?

Probably, new species are being discovered all the time by amateurs but in astronomy there is an obsession about discovering the new. There is a system established to coordinate that obsession. I think there is somewhat for diatoms too, Aquasteroids. Even when studying taxonomy around 1970, I had hu...
by apochronaut
Mon Jun 07, 2021 1:31 am
Forum: Collecting microscopes and slides
Topic: Spencer Buffalo Stereo Microscope
Replies: 3
Views: 194

Re: Spencer Buffalo Stereo Microscope

There were various permutations on that stand. I have had mostly the version with the articulating arm, a kind of quasi boom stand. One of the neat applications is to rotate the column to horizontal. Formatted in such a way those scopes make a good low power microscope for l.w.d. viewing through an ...
by apochronaut
Sun Jun 06, 2021 10:18 pm
Forum: Microscopes and optics
Topic: Is this a good objective?
Replies: 13
Views: 332

Re: Is this a good objective?

Don't give up hope on the Nikon. I will be tackling it tomorrow evening.

apologies. I confused you with another Javier on the forum.
by apochronaut
Sun Jun 06, 2021 9:11 pm
Forum: Miscellaneous
Topic: Microscopes on TV
Replies: 8
Views: 236

Re: Microscopes on TV

75RR wrote:
Sun Jun 06, 2021 2:59 pm
.
Doctor in 'Sweet Tooth' making saving the world harder on himself by using a microscope without objectives.
.
Was the script missing it's objectives too?
by apochronaut
Fri Jun 04, 2021 1:56 pm
Forum: Beginner's corner
Topic: Question about a Particular Phase Contrast Microscope
Replies: 12
Views: 343

Re: Question about a Particular Phase Contrast Microscope

Well. The Galen would have been o.k. too. I have a couple of AO, if you are interested in those.
by apochronaut
Fri Jun 04, 2021 1:54 pm
Forum: Beginner's corner
Topic: Advice to buy a Plan objective
Replies: 6
Views: 257

Re: Advice to buy a Plan objective

It also depends on the individual objective design. Some standard objectives have surprisingly flat fields.
by apochronaut
Wed Jun 02, 2021 8:48 pm
Forum: Microscopes and optics
Topic: Vanox dovetail
Replies: 13
Views: 273

Re: Vanox dovetail

Looks like this popular Polish microscopist is using a Olympus Trino on his Biolar: https://youtu.be/lSuIuUT4qKs That helps some., It seems it shouldn't be too difficult a swap, irregardless of the 1mm. It looks like he is not using PZO objectives. You probably can use many strain free 160mm object...
by apochronaut
Wed Jun 02, 2021 7:39 pm
Forum: Microscopes and optics
Topic: Vanox dovetail
Replies: 13
Views: 273

Re: Vanox dovetail

Yes, and as shown in the thread I linked, the Olympus circular dovetail is 42mm, so it's very close. Could work. People have mostly had trouble getting PZO DIC units to work on Olympus microscopes, the reverse should be easier. The Vanox head might work. But I don't know the tube length of the vano...