MicrobeHunter.com Microscopy Forum

Well, my goodness, I'm flattered for the critique . It was kind of about microscopy too.

There is a belief that is held by some , that plan is not necessary for thick preparations. However , this although not completely dumb, does not account for certain aberrations. For in addition to planarity, a plan objective offers more clarity. Not only by focusing the edge with the axis but also ...

If these silicon-based creatures had silicon eye-lenses, then they would only see in the infra-red, since silicon is essentially opaque in wavelengths shorter than say 1100nm. Then, silicon's refractive index at 1100nm is around 3.5. I wouldn't know what a good match would be, but it sure isn't air...

My guess is, they would use very similar immersion oils like us. As carbon based life forms we use materials containing every element in the periodic systems. Why should a silicon based life form behave different? Probably wouldn't be but theoretical scenarios for potential environments range from ...

That looks like a 30mm thread. The A is supposed to mean fully corrected. Auskorrigiert, if I have it spelled correctly. The reference length is 250mm.

If silicon based life existed somewhere and proceeded to the point of requiring and developing light microscopy : what would the immersion mediums be?

It is silicone immersion but if you can pull that off the image quality will be quite high with the following provisos. It is messy. I have not used that objective but there are some characteristics of the short barrel Olympus objectives that one would need to be aware of before considering using on...

Beginning in the 1960's Bausch & Lomb evolved 2 streams of compound microscopes. Those made in Rochester that evolved out of their unique Flat Field optics program and a kind of tier 2 group based on imported microscopes. The imported biological ones had 160mm optics. The most common of the latter w...

Dovetail mount ? You know this reference I guess?

https://www.alanwood.net/olympus/microscopes.html

Pete M knows a lot about DIC setups , particulary Olympus. Maybe he will chime in.

With both having a 30 watt halogen bulb, both would benefit from a little absorption of the longer wavelength part of the spectrum, so a blue filter is valuable for both. The 31 was more for student use so perhaps they just wanted to make it very straight forward. The 41 had several condenser option...

Actually, the #2110 1.30 condenser isn't that similar to the Reichert 90/1.30 condenser because it was not really intended to be used dry, as the Polyvar condenser was at .90, or for phase for that matter. The 2110 was a moderately high N.A. strain free oil immersion achromat aplanat intended for DI...

One further note about eyepieces. There are two versions of Polyvar 10X/24 eyepieces, a WPK 10X/24 and WP 10X/24. Presumably with the WP , they have removed the .4% compensation from the eyepiece and put it elsewhere. It looks like the WP were for the Polyvar 2 and as a 20mm f.o.v. version the Polyl...

The 40X .70 Plan Fluor is based on a third generation system designed for the series 400 microscopes. The Plan Achro and Plan Fluor objectives in that program were all for interference contrast. Examples of working DIC Diastars for instance are fitted with standard Plan Achros and Plan Fluors. It se...

Probably. Titanium or Zirconium flint would have by then become the option and likely. BK 7 was ubiquitous but with a high refractive index, much like flint but lower dispersion it could only have been used as a direct replacement when a high degree of other juggling of the math occurred. Interestin...

https://patents.google.com/patent/US4373785A/en?inventor=arthur+shoemaker&oq=arthur+shoemaker&page=2 A second embodiment of the 100X Planapo Reichert objective patented in 1981. Essentially it is the same 8 cell , 13 element objective but with some changes in curvatures, radii, refractive index and ...

The scans are all very helpfull to fill in the gaps. Since you have 5 - 40X objectives, I am hoping you have the 40X .70 Plan Fl Apo . Since your plan is to avoid the use of immersion with your students I have found it one of the most perfect objectives for general use. Despite having only a modest ...

https://patents.google.com/patent/US365 ... ker&page=2

This would have been carried forward for the Polyvar.

https://patents.google.com/patent/US370 ... ker&page=2

This would have been carried forward for the Polyvar.

https://patents.google.com/patent/DE233 ... ker&page=3

This would have been carried forward for the Polyvar.

I did a little further checking. Arthur Shoemaker had two patents for 10X .25 Plan 34mm parfocal objectives. They are surprisingly different but achieve very similar goals. One is no doubt the cat.# 1021 and the other maybe the later cat.# 1127. No fluorite glass in either but the 1021 at least nudg...

I'd prefer to see the thicker, inside part of those jaws fully over the condenser barrel. Can you put a metric ruler or scale across the i.d. of the sleeve and see what you get as well. Not the caliper, a scale or ruler using the 10 as zero because sometimes that first mm on tapes/rulers are not acc...

Olympus , for some reason went back to an older condenser sleeve mount for those microscopes. It's anyone's guess why but imo , the reason is pretty transparent. Their previous mount was 36.8mm. I was just trying to confirm if they used the same as previous or have they used another dimension. If yo...

37mm sleeve?

Are the centering screws not integral to the condenser on those two CX's?

According to Leica HC objectives are not backwards compatable with Delta optics. Presumably this would mean that the two former systems would most likely be incompatible too. I don't know what the incompatability details are.HC and HCX are compatable, HCX and Delta are but not HC and Delta.

CX-41 uses 1.25 according to the manual but the CX-41 can accept other objectives. Aside from fit, there are height and focal length concerns with a condenser, plus it's capability to accept an aux. lens for very wide fields. The 31 and 41 are the same frame and illumination, are they not? I am wond...

I'm sorry but I wrote that really fast out of my noggin, which might have been sleep deprived and I meant CX. so just replace the B with a C. I'm pretty sure all else is accurate. Edited for accuracy now.