Search found 4047 matches

by apochronaut
Mon Apr 19, 2021 1:08 pm
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 4
Views: 105

Re: should i buy Bresser phase contrast series 57?

It is a good idea you asked that question. My answer would be , don't. Simply because the package mentions nothing about the objectives being plan. They appear to be achromats only which sets them back in time to the early days of phase contrast and thus they will be no better, and likely worse due ...
by apochronaut
Mon Apr 19, 2021 12:03 pm
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 11
Views: 175

Re: AO 1240 phase condenser (for model 10) on 110

I would set up one of the series 10 microscopes for phase, if I were in your shoes. Spend the time to clean and properly adjust one. A push pull type cork, the kind with the plastic knob glued on will work for the trinocular cover or pick up one of the photo tubes on line. The knock off ones may sti...
by apochronaut
Sun Apr 18, 2021 4:49 pm
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 11
Views: 175

Re: AO 1240 phase condenser (for model 10) on 110

occasional 1240 condensers will slide into a series 100 yoke and locate by friction rather than by registration, since both the condenser and yoke will have registration grooves, rather than pins. the fit is tight and will work o.k. you can encourage a fit with a file. however, fit is only one of th...
by apochronaut
Fri Apr 16, 2021 12:50 pm
Forum: Illumination Techniques
Topic: Researchers create light waves that can penetrate even opaque materials
Replies: 3
Views: 107

Re: Researchers create light waves that can penetrate even opaque materials

When does the first transmitted illumination met microscope come out?
by apochronaut
Thu Apr 15, 2021 11:55 am
Forum: Microscopes and optics
Topic: Nikon Plan objective - washed out colours
Replies: 12
Views: 278

Re: Nikon Plan objective - washed out colours

No achromat has full spectrum colour correction. In fact, no objective has full spectrum colour correction. If achromats could do it, then fluorite and apochromat objectives wouldn't exist and even they are on a never ending treadmill leading to perfection somewhere near to infinity. Companies will ...
by apochronaut
Tue Apr 13, 2021 10:41 am
Forum: Microscopes and optics
Topic: Nikon Plan objective - washed out colours
Replies: 12
Views: 278

Re: Nikon Plan objective - washed out colours

All microscope objectives used properly in a coherent system made during any era of manufacturing by reputable companies have fairly equivalent performance. There are differences between them but it is quite subtle. One manufacturer might show slightly lower saturation of red for instance but have h...
by apochronaut
Tue Apr 13, 2021 1:35 am
Forum: Identification help
Topic: Mold, bacteria biofilm, parasite, or something else?
Replies: 7
Views: 4767

Re: Mold, bacteria biofilm, parasite, or something else?

I have to agree more than in part with you. Forum rules are necessary ; they aren't the problem ; what is, is the banal interpretation of them by some ; that any kind of request for i.d. on anything related to the human body is medical advice. This is a microscope forum. All kinds of samples can be ...
by apochronaut
Sun Apr 11, 2021 8:15 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 544

Re: can Phase contrast kit work WITHOUT Köhler ???

I should have added that the condenser is never oiled for phase. It works at sub 1.0 N.A. but arrives at that differently than DF.
by apochronaut
Sun Apr 11, 2021 4:09 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 544

Re: can Phase contrast kit work WITHOUT Köhler ???

There are more types of illumination than just critical or Köhler. Those are just two extremes related to how focused or defocused the illumination source is. Critical illumination requires that the illumination source be very broad and quite uniform in illumination intensity. It was the illuminatio...
by apochronaut
Fri Apr 09, 2021 10:51 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 1546

Re: PZO DIC on a BHT microscope, possible?

Uhhhhh! I am thinking. Why not just buy a Biolar microscope body? You can probably even get the nice 100 watt version with a 5 nosepiece.
by apochronaut
Thu Apr 08, 2021 2:33 pm
Forum: Beginner's corner
Topic: what is a 1x objective ?
Replies: 5
Views: 477

Re: what is a 1x objective ?

Corrected wide field.
by apochronaut
Thu Apr 08, 2021 2:31 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 675

Re: Glycerol Immersion Objectives

Presumably your monochromatic condition is close to one of the wavelengths that the objective is corrected for?
by apochronaut
Thu Apr 08, 2021 1:49 pm
Forum: Illumination Techniques
Topic: DIY phase anulus with LOMO phase objectiv
Replies: 12
Views: 631

Re: DIY phase anulus with LOMO phase objectiv

Agree with Fan. The image of your phase diaphragm should be conjugate with the phase annulus in the objective , when the condenser is focused perfectly on the sample. Where you are placing the phase diaphragms is more typical of the ideal location of a DF occlusion. Typically, phase condensers are o...
by apochronaut
Thu Apr 08, 2021 12:37 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 675

Re: Glycerol Immersion Objectives

As with so many things , there is theory and then there is empirici. There does seem to be a bit of a phobia that plagues newly hatched microscopists when it comes to oil immersion. It is a little difficult to master but immersion is immersion and outside of an extra minute or two to clean up a bit ...
by apochronaut
Wed Apr 07, 2021 12:52 am
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 675

Re: Glycerol Immersion Objectives

Quartz cover slips might be of some advantage but the conventional suppliers want a pretty penny for them. I just happen to have some tweaked water immersion objectives coming from China soon and they are sending some quartz covers and slides at the same time. I am not sure about the price but it wi...
by apochronaut
Tue Apr 06, 2021 8:04 pm
Forum: Microscopes and optics
Topic: Glycerol Immersion Objectives
Replies: 12
Views: 675

Re: Glycerol Immersion Objectives

I sometimes use a 63X 1.0 N.A. glycerin immersion planachro. It is very good but since I don't also have the compatible 100X 1.25 glycerin immersion planachro, it is a bit cumbersome. There are very few objectives that can be used with multiple immersion media, without having adjustment collars. Thi...
by apochronaut
Mon Apr 05, 2021 7:45 pm
Forum: Beginner's corner
Topic: Can someone help me understand this?
Replies: 8
Views: 393

Re: Can someone help me understand this?

They will work fine as a set. It is possible though, that in order for them to perform to perfectly planar well corrected specs. they would need eyepieces other than what you have on your microscope. Amscope buys parts and entire systems from multiple factories and jobbers, so full optical compatabi...
by apochronaut
Fri Apr 02, 2021 1:37 am
Forum: Identification help
Topic: Fungus on Pear?
Replies: 2
Views: 170

Re: Fungus on Pear?

Spores are very tiny. Just a few microns across. You may be seeing some fruiting bodies but not spores. If the fungus was black, it might be what is commonly called rhizopus rot by pomologists.
by apochronaut
Fri Apr 02, 2021 1:32 am
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 22
Views: 831

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

From the size of the oil globule or what looks like one, it seems the oil might be high in the lens column. Usually it is a smaller less noticeable contamination when it is down near the front lens. Sometimes it is hard yo see at all.
by apochronaut
Thu Apr 01, 2021 5:54 pm
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 22
Views: 831

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

There are several scenarios with such a lens. 1) the oil has invaded minimally and it simply requires cleaning of the oil , out of the lens chamber and major reduction of the oil in the correction collar and retraction cylinder. That is a fairly easy scenario and is the most likely with a modern len...
by apochronaut
Thu Apr 01, 2021 1:44 am
Forum: Beginner's corner
Topic: "Pol" objectives in macrophotography under non-polarized, incident light
Replies: 5
Views: 256

Re: "Pol" objectives in macrophotography under non-polarized, incident light

Be advised though, that Zeiss Jena objectives will be best used with eyepieces providing the designed corrections for them.The correct Zeiss Jena eyepieces are the obvious choice but eyepieces mfg. with the same or similar corrections might work well enough : you won't know that until you try them o...
by apochronaut
Wed Mar 31, 2021 7:17 pm
Forum: Microscopes and optics
Topic: A small tip for users of Bausch & Lomb.
Replies: 10
Views: 488

Re: A small tip for users of Bausch & Lomb.

Nice find. n Great system. They finally abandoned it in about 1975, when they introduced spring loaded objectives, which are parfocalized by rotating the cam against the spring.
by apochronaut
Wed Mar 31, 2021 10:27 am
Forum: Illumination Techniques
Topic: Sony phase contrast patent application.
Replies: 3
Views: 201

Re: Sony phase contrast patent application.

Who knows what they are up to . Kodak patented all kinds of microscope related patents.
https://patents.google.com/patent/US20150309296A1/en

They seem to be focused on detection. Maybe Sony will bring out a new version of Clue?
by apochronaut
Tue Mar 30, 2021 9:40 pm
Forum: Illumination Techniques
Topic: Sony phase contrast patent application.
Replies: 3
Views: 201

Sony phase contrast patent application.

In looking for some patents it appears there may be a new player in microscope manufacturing. Sony has a patent application for what appears to be a high resolution phase contrast technology, overcoming the resolution limitations of conventional phase. Unless, the purpose is for in house use. Compan...
by apochronaut
Tue Mar 30, 2021 9:30 pm
Forum: Microscopes and optics
Topic: Condenser corrections aplanatic, achromatic, etc. -- which planes are they referring to?
Replies: 8
Views: 419

Re: Condenser corrections aplanatic, achromatic, etc. -- which planes are they referring to?

Is the image of the condenser iris not further corrected in the objective, though? Incidentally, Milton Sussman patented two abbe aspheric condensers within a year of each other. One is a two element and the other a 4 element in 2 groups.
by apochronaut
Tue Mar 30, 2021 9:17 pm
Forum: Camera systems and imaging
Topic: Michrome camera
Replies: 9
Views: 380

Re: Michrome camera

Yes. I guess what I am indecisive about is not knowing how fast is fast when it comes to biological samples? ...and how that relates to a frame rate of 53 fps., at which speed the increased resolution of the 20 mpx. model is still relavent. The global shutter model achieves slightly better resolutio...
by apochronaut
Tue Mar 30, 2021 8:16 pm
Forum: Microscopes and optics
Topic: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined
Replies: 22
Views: 831

Re: I received my Nikon CFN PlanApo 60/1.40 Oil, is completely ruined

My comments about ebay were more in general, not directed at you. Some people get burned on ebay because they don't ask the right questions. In the case of this objective. If the seller described it as in excellent condition, how would they know, since they clearly never looked through it? The listi...
by apochronaut
Tue Mar 30, 2021 6:16 pm
Forum: Camera systems and imaging
Topic: Michrome camera
Replies: 9
Views: 380

Re: Michrome camera

I am willing to try the in camera system. I need a video camera of this type anyway. The cameras themselves seem good, at a 400.00 price point and the stitching/stacking feature a nice bonus. I can see a future where add on stacking programs are as obsolete as roll film and everything is in camera. ...