MicrobeHunter.com Microscopy Forum

Hello. I was wondering if the stage from the AO Fifty Phase Microscope could be attached to the body of the Series 10. I did some research and found that the stage of the Series 10 comes off with a lever, but I was wondering if the stage further comes apart in a way where the stage of another could...

You will need AO Spencer cat. 1240 condenser with a top lens such as cat. 1242. And AO 34mm (short) parfocal infinity objectives. To add to that. All of the AO and Reichert Buffalo made phase condenser bodies for infinity corrected microscopes except one, had the same thead to receive the condenser...

Hello. I have recently found crystalline structures in a fecal sample I took. I repeatedly found them in the sample. I have taken more samples lately and have not been able to found them. I have determined that it was not the glass as they moved with the current at times. Does anyone know what these...

Hello. I have found numerous Series 10 AO microscopes for sale, however have not been able to find any equipped with Phase Contrast. Does anyone know the necessary parts to convert the non-phase model into a phase contrast one? Any help is appreciated!

During the early years of the development of phase contrast systems, manufacturers of those systems experimented a lot with various types , locations and dimensions of retardation plates in the phase objectives( phase rings). This led to certain types of plates forming images that caused certain fe...

Hello. Is there a difference between a Dark Phase objective and a phase objective, or does this mean that it can be used for darkfield and phase contrast. I have seen this on old AO objectives. Any help is appreciated.

Thank you for the reply. Do you think that the base of the Fifty Phase could come off, I know that the Series 10's base can. I looked and could not find anything that would indicate that it would come off, but wanted to ask. Also, do you know of any different heads that could fit onto the Fifty Pha...

Hello. I was wondering if the stage from the AO Fifty Phase Microscope could be attached to the body of the Series 10. I did some research and found that the stage of the Series 10 comes off with a lever, but I was wondering if the stage further comes apart in a way where the stage of another could...

Hello. I was wondering if the stage from the AO Fifty Phase Microscope could be attached to the body of the Series 10. I did some research and found that the stage of the Series 10 comes off with a lever, but I was wondering if the stage further comes apart in a way where the stage of another could ...

Hi, MicroscopyLearning. It's Bacterial Endospore. It looks like a bright bead in Phase Contrast. ==> https://www.dreamstime.com/illustration/endospores.html Here's my old video. Oe_AO1kQ8y8 Thanks, this is quite helpful. I looked at the Wikipedia entry for it (https://en.wikipedia.org/wiki/Endospor...

Hello. I was recently observing a fecal sample and came across these very interesting kind of what I assume are bacterium. I wanted to see if anyone has any ideas on what this could be. Thank you for any help you can give. There is also an interesting kind of crystal in the video. If anyone has any ...

Hello. Recently I have been wondering how to stain the chromosomes of a cell. I have seen multiple sources saying to use the stain Safranin. How would you recommend applying this stain, should it be diluted first and then applied? The safranin I use is a 1 percent aqueous solution. Also, does anyone...

Do you have any recommendations on what to view with a phase contrast microscope, ... Do you have any recommendations for samples to view? Phase contrast is of limited help to view bacteria. This has been explained to you before. An easy example specimen is the following: Put some large thick slice...

wabutter wrote: ↑

Tue Dec 28, 2021 8:00 pm

I used to have a couple of different types that were essentially homemade. I'll see if I can find one in an old tool kit and take a picture. One was wooden, with a soft metal strap, that other was all metal.

Thank you. Do you have any recommendations on how to make one?

Hello. Does anyone have recommendations for good bacteria specimens? I am using phase contrast.

You will need an objective wrench in order to get them off. An objective wrench in similar in design to a jar opener, only the scale is brought down to fit into the nosepiece and objective. I have looked and only found a few, but I am not sure if they are compatible or the same as this kind of obje...

apochronaut wrote: ↑

Tue Dec 28, 2021 6:02 pm

Yes , objectives are standard R.M.S. threads.
There are many manuals available here. https://user.xmission.com/~psneeley/Personal/Microscope.htm

Do you have to take out a screw, on mine I have found it hard to take the objectives out. What would you recommend doing?

Hello. Does anyone know where to find manuals for older American Optical microscopes. Also, does anyone know if the objectives are removable on them?

Just a quick and dirty picture of what you could expect. The picture shows some Cocci with gram-staining. Bacteria.jpg Do you have any recommendations on what to view with a phase contrast microscope, while I would like to be able to view bacteria with bright-field, I am also fine with doing it wit...

Ok. I see what you mean now, but doesn't a starter culture sometimes contain yeast sometimes though? Yeast doesn't produce yogurt. From what I have read some yoghurts do contain yeast, but I am not sure if the starter culture contains it. What do you think? Also, about how high should the resolutio...

What do you mean by a yoghurt culture? Do you mean the bacteria sample or do you mean a yoghurt starter culture? A yogurt starter culture. It contains little more than bacteria typically streptococcus thermophilus and sometimes lactobacillus delbrueckii. Activate the culture in warm water with some...

Will diluting the specimen into a glass of water than piping out the water onto the slide work? Also, I saw in a video that Microbehunter did not dilute his sample and rather put it on and looked at the edges of the sample. Which way would you recommend? The challenge is to clean the specimen from ...

Do you live near the ocean? I find tons of oscillatoria and other cyanobacteria in the stinky, mucky shores of the SF bay. Where I live I cannot really access the ocean very well, but there are some streams that I could go to. Do you think that there is any substantial amount of cyanobacteria in th...

No, with yoghurt (and make sure it is yoghurt that still has live cultures) I would go the other direction, to thin and separate the yoghurt enough to see individual bacteria. That is, put a drop of yoghurt on the slide, then dilute it with a drop of water before placing the slip cover on top. With...

Like others have said, it's not difficult to grow some cyanobacteria. If it ever helps, though, you can also find places where they've grown into visible mats and films. I have had luck finding them at the edges of marshes and lakes, where they are generally darker than green algae, and whenever I ...

I believe anywhere there is light and the oxygen levels are lower, you will be likely to find some, so slow moving warmer water or anything poorly aerated. Easiest would probably to put a jar of water with a lid in a windowsill for a couple of weeks, should see it as blue-green patches. Many small ...

Are you presently observing protists? If so, using Don's technique, you should see bacteria wherever there are protists, with the 40x or higher objective. Depth of field will be shallow so you don't want much water under the slip cover--you may have most luck at the point that the water begins to d...

Yeah, we don't yet have electoplasmic contrast in the arsenal, maybe someday :D But really, even the most humble compound microscope of today is better than the microscopes that early researchers used to make important discoveries. Van Leeuwenhoek's microscope used a tiny glass sphere as the only o...

Hello. Does anyone have a recommendations on where to find cyanobacteria. What are some common signs that might indicate their presence? Have a careful look into your bathroom. Are there any red or blue patches in the shower? Yes. I think I know what you are talking about. Currently I do not see an...

BramHuntingNematodes wrote: ↑

Mon Dec 20, 2021 6:35 pm

For living bacteria use ectoplasmic contrast to see the tiny bits of their souls

What do you mean by this? I am not familiar with ectoplasmic contrast. Is this an optical technique for viewing them?