Search found 91 matches
- Mon Feb 19, 2024 1:27 am
- Forum: Digital processing
- Topic: Re: ZEISS ZEN Lite (Blue)
- Replies: 1
- Views: 3233
Re: ZEISS ZEN Lite (Blue)
Hmm, maybe long dead thread... but what about ZEN Blue?
- Thu Dec 21, 2023 3:03 am
- Forum: Beginner's corner
- Topic: Best size objective for Pond life?
- Replies: 36
- Views: 58197
Re: Best size objective for Pond life?
I wonder, if the modern electrophysiology objectives at 20x and 40x would be the best option - optimized for water immersion, long working distances, quite high NA.
- Tue Nov 21, 2023 3:45 am
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Re: Interchangeable DIC prisms (shear values for different manufacturers)
:D Puzzle is all quite simple - laminate frame stressed Sanderson prism between glass plates. Remove frame. Trim excess. Or use suitable laser to ablate internal matrix on the edge areas to create expansion stress - have not yet tested this. I was trying to see, if anyone has more interesting approa...
- Sun Nov 19, 2023 12:28 pm
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Re: Interchangeable DIC prisms (shear values for different manufacturers)
To drive further the discussion - edge view:
- Mon Nov 06, 2023 11:56 pm
- Forum: Microscopes and optics
- Topic: HELP: IDENTIFY STRANGE APPARATUS WITH OBJECTIVE 10X AND FILTERS
- Replies: 31
- Views: 31814
Re: HELP: IDENTIFY STRANGE APPARATUS WITH OBJECTIVE 10X AND FILTERS
The methacrylate prism reminded me plastic scintillators where the plastic matrix carries fluorescent material responding to ionizing radiation excitation. Cherenkov/scintillaton characterization maybe? UV block with yellow filters etc. Just pure speculation... Check, if it is slightly more radioact...
- Mon Nov 06, 2023 5:10 am
- Forum: Microscopes and optics
- Topic: HELP: IDENTIFY STRANGE APPARATUS WITH OBJECTIVE 10X AND FILTERS
- Replies: 31
- Views: 31814
Re: HELP: IDENTIFY STRANGE APPARATUS WITH OBJECTIVE 10X AND FILTERS
Check, if it is slightly more radioactive compared to background. The prism might be scintillator?
- Mon Nov 06, 2023 4:37 am
- Forum: Microscopes and optics
- Topic: DIC Prism Design
- Replies: 18
- Views: 8905
Re: DIC Prism Design
I missed one auction on Ebay for all 3 volumes, have not seeked actively for the 2-nd and 1-st after that. I believe there is already scan for vol. 1 and 2 but not available in public archives. On the other hand, a friend now sends me two "Advanced Light Microscopy vol. 2 M. Pluta1989". When they ar...
- Fri Nov 03, 2023 9:38 pm
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Re: Interchangeable DIC prisms (shear values for different manufacturers)
This is UV filter casing from HOYA. One can see the residual stresses resulting from the injection molding. There are multiple methods for creating the stress gradients for organic materials, some seem to be giving reasonable results. Stresses2.jpg What considers the Nomarski vs Wollaston prisms and...
- Sat Oct 14, 2023 5:26 pm
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Re: Interchangeable DIC prisms (shear values for different manufacturers)
Those are pretty nice finds, thank you! From: https://www.researchgate.net/publication/236224633_Quantitative_orientation-independent_differential_interference_contrast_microscope_with_fast_switching_shear_direction_and_bias_modulation From: https://www.researchgate.net/publication/287716624_Differe...
- Sat Oct 14, 2023 5:24 pm
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Re: Interchangeable DIC prisms (shear values for different manufacturers)
Basically Sanderson prisms without the external stress frame, pretty much any method works as long the correct stress gradient is created in the polymer.
Scarodactyl wrote: ↑Sat Oct 14, 2023 6:06 amI am not familiar with frameless stress gradient prisms. How do they work?
- Sat Oct 14, 2023 5:11 am
- Forum: Illumination Techniques
- Topic: Interchangeable DIC prisms (shear values for different manufacturers)
- Replies: 11
- Views: 7153
Interchangeable DIC prisms (shear values for different manufacturers)
Are there DIC prism shear values stated somewhere for the Leica, Nikon, Zeiss, Olympus? Considering the frameless stress-gradient DIC prisms could be manufactured quite cheaply, it may be beneficial to try to gather list of different manufacturers DIC prism shear values as a reference. Frames for th...
- Mon Jul 10, 2023 1:33 am
- Forum: Microscopes and optics
- Topic: Let’s talk about Delamination
- Replies: 30
- Views: 55755
Re: Let’s talk about Delamination
How is the modern Zeiss stuff doing, any known problems?
- Mon Mar 06, 2023 12:57 pm
- Forum: Illumination Techniques
- Topic: Micromanager controllable LED drivers (preferably flicker free)
- Replies: 2
- Views: 2391
Re: Micromanager controllable LED drivers (preferably flicker free)
Thank you for the reference, seem to be nice products there.
Macro_Cosmos wrote: ↑Sun Mar 05, 2023 10:46 amMost Lumencor light engines (fancy term for LED) can be controlled with MM.
Not cheap, but great product and a great team are behind it.
- Sat Feb 25, 2023 8:57 am
- Forum: Illumination Techniques
- Topic: Micromanager controllable LED drivers (preferably flicker free)
- Replies: 2
- Views: 2391
Micromanager controllable LED drivers (preferably flicker free)
Probably I have not made good enough search... but please kindly direct me towards Micromanager controllable multichannel LED drivers if you know one (reasonable price would be a nice bonus). I have seen many interesting expensive big 4 products (seriously expensive) and even more interesting possib...
- Mon Jan 30, 2023 5:30 am
- Forum: Microscopes and optics
- Topic: Suggestions for a diy xy mechanical stage for an inverted scope
- Replies: 7
- Views: 2445
Re: Suggestions for a diy xy mechanical stage for an inverted scope
Offset modified servospline may be interesting starting point, if ready made cross roller motorized stage is tending to be too spicy: https://www.youtube.com/watch?v=wLTf87E9Wno Also, the final reports may offer good insights: https://bmedesign.engr.wisc.edu/projects/f20/motorized_stage https://bmed...
- Sat Jan 28, 2023 5:24 pm
- Forum: Microscopes and optics
- Topic: Photomasks as optics testing tool
- Replies: 0
- Views: 1584
Photomasks as optics testing tool
I stumbled recently on this and similar items on Ebay:
https://www.ebay.com/itm/125616622774
Seems to be quite decent way to evaluate microscope optics, I assume those are manufactured by electron litography.
https://www.ebay.com/itm/125616622774
Seems to be quite decent way to evaluate microscope optics, I assume those are manufactured by electron litography.
- Thu Jan 26, 2023 12:39 am
- Forum: My microscope
- Topic: So sad
- Replies: 13
- Views: 8145
Re: So sad
Just tell her, that it could be also 1 microscope and 16 women. That usually settles it.
- Wed Jan 25, 2023 4:36 pm
- Forum: Microscopes and optics
- Topic: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
- Replies: 14
- Views: 5308
Re: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
By the way, variable spherical aberration correction is also common in camera macro lenses, that have a further "floating" lens that moves when focusing: such that when the lens is focused near, the spherical aberration (and maybe others) are corrected for that focal distance. Similar to the "corre...
- Wed Jan 25, 2023 3:46 pm
- Forum: Microscopes and optics
- Topic: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
- Replies: 14
- Views: 5308
Re: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
A spherical aberration adjustable compensator, that's not a bad thing after all. (But the same result can also be achieved by simply adjusting the tube length :? or the sensor position in an infinity system) I'm dreaming since a couple of years of an "adjustable compensating eyepiece" for chromatic...
- Mon Jan 23, 2023 11:35 pm
- Forum: Miscellaneous
- Topic: cigars and microscopy
- Replies: 7
- Views: 2493
Re: cigars and microscopy
How about cognac to go with it?
- Thu Jan 19, 2023 6:49 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
- Replies: 9
- Views: 3786
Re: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
Spatial filtering would make it clear - pinhole in foil will give good clean spherical wavefront (needle used on aluminium foil resting on ceramic tile gives quite good small pinholes). It may be, that diode laser without optics is actually better for this kind of evaluation but I have not tried it.
- Wed Jan 18, 2023 7:45 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
- Replies: 9
- Views: 3786
Re: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
Slightly expanded laser shows well common faults like damage to front lens. Ideally the projected image is homogeneous circle (assuming laser beam is clean), free from aberrations from damage or dirt. Not an ideal or exhaustive test but quick and simple. On the image the beam expander (laser source ...
- Mon Jan 16, 2023 10:47 am
- Forum: Microscopy accessories
- Topic: Test slide made by Optical Insights Spectral Imaging Solutions
- Replies: 6
- Views: 3233
Re: Test slide made by Optical Insights Spectral Imaging Solutions
Interesting technology. There is quad version here:
https://www.biovis.com/bodies/automatio ... ights.html
https://www.ebay.com/itm/364083343014
https://www.biovis.com/bodies/automatio ... ights.html
https://www.ebay.com/itm/364083343014
- Sun Jan 15, 2023 2:20 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
- Replies: 9
- Views: 3786
Re: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
Thank you, this is bit high priced there.
- Sun Jan 15, 2023 5:48 am
- Forum: Microscopes and optics
- Topic: Optical Microscope Technology in Japan
- Replies: 5
- Views: 1691
Re: Optical Microscope Technology in Japan
Nice overview publication, lot of info condensed.
- Fri Jan 13, 2023 8:00 am
- Forum: For forum members who want to buy and sell equipment
- Topic: WTB: Zeiss C-Apochromat 63x/1.20 W Corr
- Replies: 9
- Views: 3786
WTB: Zeiss C-Apochromat 63x/1.20 W Corr
Maybe there is someone wanting to part with one. I would like to see the green laser transmission projection to evaluate the condition before buy.
Thank you!
Thank you!
- Fri Jan 13, 2023 7:49 am
- Forum: Illumination Techniques
- Topic: Shrink Plastic As Rheinberg Filters
- Replies: 1
- Views: 2232
Re: Shrink Plastic As Rheinberg Filters
For DIY microfluidics the material is quite good.
biofilmer86 wrote: ↑Sun Nov 13, 2022 5:23 amRemember Shrinky Dinks? I'm not sure if using heat-shrunk polystyrene would make good rheinberg filters, but it might make decent darkfield and oblique filters. Give me your thoughts.
- Mon Jan 09, 2023 1:43 pm
- Forum: Microscopes and optics
- Topic: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
- Replies: 14
- Views: 5308
Re: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
Truly enjoyable references, both the Apochronaut-s technical/historical insight and the reference to the H. J. Margolis thread, thank you. It would be interesting to experiment on the same principles as InFocus provides with the tunable lenses like those: https://www.edmundoptics.com/f/optotune-manu...
- Sun Jan 08, 2023 4:35 am
- Forum: Microscopes and optics
- Topic: InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
- Replies: 14
- Views: 5308
InFocus™ Dynamic Optical Focusing Systems (tubelens on steroids)
Interesting, seems to be really nice on paper. Although color correction mandates separate versions for Zeiss, Leica etc. From the documentation: • Imparts DYNAMIC OPTICAL FOCUSING to Almost ANY Infinity-Corrected Microscope • Corrects Spherical Aberration BETTER than Correction Collared Objectives ...
- Sat Nov 19, 2022 1:05 am
- Forum: Microscopes and optics
- Topic: 20 mm working distance air objective, 10x, 0.50 NA
- Replies: 10
- Views: 2712
Re: 20 mm working distance air objective, 10x, 0.50 NA
The original source seems to order small batches of them, have no idea, what the costs are, there was talk about batch two last time I looked.