MicrobeHunter.com Microscopy Forum

Thank you for the reference, seem to be nice products there.

Macro_Cosmos wrote: ↑

Sun Mar 05, 2023 10:46 am

Most Lumencor light engines (fancy term for LED) can be controlled with MM.
Not cheap, but great product and a great team are behind it.

Probably I have not made good enough search... but please kindly direct me towards Micromanager controllable multichannel LED drivers if you know one (reasonable price would be a nice bonus). I have seen many interesting expensive big 4 products (seriously expensive) and even more interesting possib...

Offset modified servospline may be interesting starting point, if ready made cross roller motorized stage is tending to be too spicy: https://www.youtube.com/watch?v=wLTf87E9Wno Also, the final reports may offer good insights: https://bmedesign.engr.wisc.edu/projects/f20/motorized_stage https://bmed...

I stumbled recently on this and similar items on Ebay:

https://www.ebay.com/itm/125616622774

Seems to be quite decent way to evaluate microscope optics, I assume those are manufactured by electron litography.

Just tell her, that it could be also 1 microscope and 16 women. That usually settles it.

By the way, variable spherical aberration correction is also common in camera macro lenses, that have a further "floating" lens that moves when focusing: such that when the lens is focused near, the spherical aberration (and maybe others) are corrected for that focal distance. Similar to the "corre...

A spherical aberration adjustable compensator, that's not a bad thing after all. (But the same result can also be achieved by simply adjusting the tube length :? or the sensor position in an infinity system) I'm dreaming since a couple of years of an "adjustable compensating eyepiece" for chromatic...

How about cognac to go with it?

Spatial filtering would make it clear - pinhole in foil will give good clean spherical wavefront (needle used on aluminium foil resting on ceramic tile gives quite good small pinholes). It may be, that diode laser without optics is actually better for this kind of evaluation but I have not tried it.

Slightly expanded laser shows well common faults like damage to front lens. Ideally the projected image is homogeneous circle (assuming laser beam is clean), free from aberrations from damage or dirt. Not an ideal or exhaustive test but quick and simple. On the image the beam expander (laser source ...

Interesting technology. There is quad version here:

https://www.biovis.com/bodies/automatio ... ights.html
https://www.ebay.com/itm/364083343014

Thank you, this is bit high priced there.

Nice overview publication, lot of info condensed.

Maybe there is someone wanting to part with one. I would like to see the green laser transmission projection to evaluate the condition before buy.

Thank you!

For DIY microfluidics the material is quite good.

biofilmer86 wrote: ↑

Sun Nov 13, 2022 5:23 am

Remember Shrinky Dinks? I'm not sure if using heat-shrunk polystyrene would make good rheinberg filters, but it might make decent darkfield and oblique filters. Give me your thoughts.

Truly enjoyable references, both the Apochronaut-s technical/historical insight and the reference to the H. J. Margolis thread, thank you. It would be interesting to experiment on the same principles as InFocus provides with the tunable lenses like those: https://www.edmundoptics.com/f/optotune-manu...

Interesting, seems to be really nice on paper. Although color correction mandates separate versions for Zeiss, Leica etc. From the documentation: • Imparts DYNAMIC OPTICAL FOCUSING to Almost ANY Infinity-Corrected Microscope • Corrects Spherical Aberration BETTER than Correction Collared Objectives ...

The original source seems to order small batches of them, have no idea, what the costs are, there was talk about batch two last time I looked.

Interesting publication, >4 (9?) mm^2 FOV and is of fully open design - no strings attached. Nice! "The design of the objective is not patented, and it will not be patented in the future. All designs originating in this report are free for reuse, no licensing or material transfer agreements are requ...

Really nice repo: "... contains code for designing, analyzing, and carrying out multicolor structured illumination microscopy experiments based on a digital micromirror device (DMD-SIM), including DMD simulation code, DMD pattern generation, SIM reconstruction and instrument control. It also include...

About the devils and details: Texas Instruments DLP evaluation kits have image offset 100% - for symmetric projection to field diaphragm plane one needs to reposition projection lens and preferably scale the projection image size as necessary. Photographic tilt shift lenses are useful ready made sol...

As expected, someone has already realized quite nice example of optical setup with the DLP4710 EVM:

http://isopack.blogspot.com/2022/03/ima ... scope.html

About the SIM image stack processing - I have yet to try it but there is free to use machine learning code up on Gitlab and a nice 2021 publication going with it: ML-SIM https://github.com/charlesnchr/ML-SIM ML-SIM: universal reconstruction of structured illumination microscopy images using transfer...

Time to sell both and get a really good microscope ?

Try acetone/IPA mix and relatively aggressive scrape with sharpened wooden stick on one edge - if it is residue, it starts to move and there is not much to lose.

Solidified immersion oil?

What considers suitable fluorescence cameras - cheap cooled astronomy cameras seem to be beating 5 year old 12k professional sCMOS camera on readout noise and sensitivity. Sure, relatively low frame rate but this is more the question of sensor resolution. IMX533 camera - cooled, mono and quite remar...

Really nice video on SIM principles, by David Agard: https://www.youtube.com/watch?v=i73HhpLJrqs Basically it is boiling down in information gathering on wavefront, increasing NA with tricks beyond what is possible with just glass shaping in objective. Appears as simple as changing the light source ...

From Leica DMR broshure, it may be the the condenser optical scheme, the massive front element seems quite similar:

Zeiss West Grey WL with complete Jamin-Lebedeff interference contrast set
Where it is located and what you would consider for it?