MicrobeHunter.com Microscopy Forum

Bruce Taylor wrote: ↑

Wed Apr 07, 2021 12:18 pm

Yup, a rotifer. It's Taphrocampa, I believe...kind of an animated concertina. The last thing is Synura.

Still searching for the elusive tardigrade then. Thanks gentlemen

1. A ciliate called Spirostomum 2. Tardigrade 3. Don't know 4. A ciliate from the Chilodonellidae family 5. Don't know There was only 4 things :D That's so wild that the worm like creature is unicellular, there were a few approaching half a mm in length. And I finally found my first tardigrade. I'l...

I ventured out to get some totally new samples today, I found a really good bunch of things in my first drop from a local duckpond.

guesses
1) Euglenoid
2)tardigrade
3)some kind of ciliate
4) I honestly have no clue, looks like an anenome

we have all looked,.but who is man enough to admit it!

The word "heterotrich" refers to the two types of ciliary structures on these guys (not their uneven distribution). They have composite cilia (polykinetids) arranged in the form of "adoral membranelles", partially encircling the oral region; and they also have simple somatic cilia, uniformly distri...

Blepharisma is a heterotrich, based on the meaning of the words I'm guessing that means uneven distribution of cilia. What then does hypotrich mean?

noticed this guy, somewhat teardrop shaped with prominent cilia around the bucal cavity. Any ideas?

http://office.shivs.org:5080/ciliate5.mp4

Hi Guys, I found these two hypotrich attached, unfortunately I didnt see anything before, and I couldnt see what would have happened as my slide dried out. They eventually separate, and then looking at one of them it appears it has two macronuclei ? Any idea what's going on here? http://office.shivs...

I love it, I get a ton of these from moss samples. Keep up the good work!

Shivs, what you're describing is called "oblique Illumination." Since your microscope contains a filter holder, you can create oblique filters fairly easily to consistently reproduce this effect at varying magnifications. Here's some more information on how oblique illumination works: https://www.m...

I wonder if you could get one of these used ?


https://singularityhub.com/2009/12/15/h ... -home-use/

I made a quick video to show this better. its pretty neat

http://office.shivs.org:5080/3.mp4

Just took some soil and placed in a petri dish, added some water. I tilted the dish and sucked off some of the somewhat clear liquid and put it on a slide. This object is roughly 250um length and doesn't appear to move of its own accord , other than that I have no idea.

75RR wrote: ↑

Fri Mar 26, 2021 9:45 pm

.
I think you might get more feedback if you provided some details.

Knowing what microscope, objectives, slide preparation and illumination technique you used would help.

ok, thanks for the feedback. I just got a new camera so Ill take some more pics and make sure to note everything down.

just a correction on this, it appears the ring around the filter holder is partially obscuring the light path. Still not sure what this is caused by.

I accidentally noticed today that putting my blue filter partially on adds a depth to the image, is this a known technique? What causes this phenomenon?

Now that I have a half decent scope, I am trying to get some decent images. I am still trying to learn how to light specimens and getting the right balance of resolution and contrast. Please have a look and let me know what you think.

Thanks for all the info, I also found these bulbs:
https://www.bulbs.com/product/ESB-FHE

hans wrote: ↑

Thu Mar 25, 2021 6:30 am

Camera software implementation of color temperature seems a bit suspect?

No clue, I'm using the omax software, I have a new amscope cam on the way but I think it uses the exact same software.

3350K is still noticeably yellow to the eye but hopefully you see an improvement. What white balance setting were you using when you got the really yellow-looking photo? Does the camera software have a white balance mode where you manually set the color temperature? heres a comparison with default ...

FYI, I swapped the bulb with the spare in the box.. exact same. Not sure what crappy bulbs they are including. Hopefully the ones I ordered come soon.

Great topic. For me the big mystery is how tiny unicellular beings without eyes, ears, or brains can behave so much like intelligent animals. Somehow they manage to find food, evade predators (sometimes), extricate themselves from tight spots (eventually), reproduce, and sometimes even seem to have...

thanks, grabbed these
https://www.amazon.ca/dp/B015OTJTUS?psc ... yp_imgToDp

might take 2-3 weeks to get here so Ill live with these ones.

the bulb is a 20w 6v default that was in the scope. There is a 2nd replacement bulb but not sure if I want to try changing it quite yet.

thanks greg, I just adjusted the color temp in the software and it looks great.... looking through the lenses is still super yellow but im most concerned with how photo and video will turn out.... This thing is like night and day compared to the other scope I had.. now just waiting for the new cam, ...

Hi Guys, new scope arrived, such an upgrade to my crappy omax. I am a bit concerned with how yellow it appears. I have tried using the blue filter and it appears closer to normal but still not quite right. I knew halogen bulbs were supposed to be yellow but this is quite pronounced 0002.jpg 0001.jpg

https://www.amscope.com/cameras/10mp-usb2-0-microscope-digital-camera-software.html ok cool, I just bought the 18MP version because I do alot of video. I needed the higher frame rate. Yeah my framerate sucks lol I am just mainly doing pictures tbh If you lower the resolution in the software the fra...

LeonhardEuler wrote: ↑

Wed Mar 24, 2021 5:32 am

Shivs wrote: ↑

Wed Mar 24, 2021 5:27 am

which camera are you using again?

https://www.amscope.com/cameras/10mp-us ... tware.html

ok cool, I just bought the 18MP version because I do alot of video. I needed the higher frame rate.

which camera are you using again?

Well that's the first time I've actually helped anyone :) I just started microscopy about a month ago so I'm glad I can contribute. Link for cheap xantham gum below. 2.5g of powder to 125 ml of water seems to be a good amount, if you want them to be not moving maybe a bit thicker. https://www.amazon...