Search found 105 matches

by chrisimbee
Tue Jun 29, 2021 5:33 am
Forum: Pictures and Videos
Topic: "baby" physarum polycephalum from spores
Replies: 1
Views: 845

"baby" physarum polycephalum from spores

Hello, 3 weeks ago I put spores of physarum on agar gelose 1% (with no nutrive elements) with a thin layer of water. I could see the flagelate and amiboid forms for days... a few days ago i added 1 or 2 oat flakes... and today I could see the "newly born" physarum : they are tiny plasmode form for t...
by chrisimbee
Fri Jun 11, 2021 3:29 pm
Forum: Pictures and Videos
Topic: giant chromosomes of blood worms
Replies: 11
Views: 3378

Re: giant chromosomes of blood worms

I'm living in France.. so i dont know about the "Lochoptera dubia" but i'll ask the entomology dept at work. And about the carcinogeny of acridine orange can you give any data and sources ? And aceto -orcein is hard to buy and also pretty expensive. The amount needed of acridin orange was really rea...
by chrisimbee
Fri Jun 11, 2021 7:25 am
Forum: Pictures and Videos
Topic: giant chromosomes of blood worms
Replies: 11
Views: 3378

Re: giant chromosomes of blood worms

So I tried with acetic acid 100% alone and then I rince and dye with gentian violet (crystal violet). It works but it shows more blurring of the definition... 21 juin 21 chromosome chironome fixation acétique violet x20 2.jpg 21 juin 21 chromosome chironome fixation acétique violet x20 3.jpg 11 juin...
by chrisimbee
Fri Jun 11, 2021 6:11 am
Forum: Pictures and Videos
Topic: giant chromosomes of blood worms
Replies: 11
Views: 3378

Re: giant chromosomes of blood worms

11 juin 21  chromosomes chiironome x40 epifluo.jpg
11 juin 21 chromosomes chiironome x40 epifluo.jpg (84.66 KiB) Viewed 3342 times
11 juin 21  chromosome chironome ++ x16.jpg
11 juin 21 chromosome chironome ++ x16.jpg (76.14 KiB) Viewed 3342 times
11 juin 21 chromosome chironome x40  bis.jpg
11 juin 21 chromosome chironome x40 bis.jpg (60.46 KiB) Viewed 3342 times
So in conclusion : the fixation - precipitation is essential for good results :

now i'm trying to find out if the alcool alone would work..
by chrisimbee
Fri Jun 11, 2021 6:09 am
Forum: Pictures and Videos
Topic: giant chromosomes of blood worms
Replies: 11
Views: 3378

Re: giant chromosomes of blood worms

In fact I've just found out after a fex trial the missing important information : None of the protocols mention FIXATION and i'm going to show you the effect of the addition of a few drops of a mix of alcool-acetique acide : its immediat : the DNA precipitate and the chromosomes become visibles even...
by chrisimbee
Thu Jun 10, 2021 8:09 pm
Forum: Pictures and Videos
Topic: giant chromosomes of blood worms
Replies: 11
Views: 3378

giant chromosomes of blood worms

Hello, I 've found out that there was a protocol to observe the giant chromosomes présent inside the nucleus of the salivary glands of blood worms ( larvae of chironomes). I wasnt successfull in dyeing them properly yet with methylen blue or toluidin blue so I tried with orange acridine under Uv-blu...
by chrisimbee
Thu Jun 10, 2021 12:08 pm
Forum: Pictures and Videos
Topic: group of diatoms with a swarm of algae..
Replies: 1
Views: 1430

group of diatoms with a swarm of algae..

Hello,

here is a video from the group of diatoms.. there were surrounded by "flying" algae that couldnt be taken in photo properly with my Nikon. This video was taken with my astro cam (ZWO ASI 120MC) :)

set the resolution of youtube at max..

https://youtu.be/He_M_U5KSW4

Chris...
by chrisimbee
Tue Jun 08, 2021 10:24 pm
Forum: Pictures and Videos
Topic: group of diatoms..
Replies: 1
Views: 901

Re: group of diatoms..

08 juin 21 groupe de diatomé x40 ZWO ASI.jpg
08 juin 21 groupe de diatomé x40 ZWO ASI.jpg (107.03 KiB) Viewed 899 times
Chris
by chrisimbee
Tue Jun 08, 2021 10:24 pm
Forum: Pictures and Videos
Topic: group of diatoms..
Replies: 1
Views: 901

group of diatoms..

Hello, here is a little groupe of diatoms that i found at the bottom of some water sample of the garden tonight.. 08 juin 21 groupe de diatomée algues.jpg 08 juin 21 groupe de diatomées x40.jpg 08 juin 21 groupe diatomé epifluo.jpg 08 juin 21 groupe de diatomée x40 epi.jpg 08 juin 21 groupe de diato...
by chrisimbee
Mon Jun 07, 2021 8:10 pm
Forum: Illumination Techniques
Topic: First try at Fluorescence!
Replies: 7
Views: 4104

Re: First try at Fluorescence!

this is really pretty good for a first but I think you should try a filter of a bit deeper yellow to get rid of most of blue light and enhance the fluorescence.

Try with some moss or pond alguae as they fluoresce in bright red :)
by chrisimbee
Mon Jun 07, 2021 12:18 pm
Forum: Pictures and Videos
Topic: alguae
Replies: 1
Views: 945

Re: alguae

07 juin 21 algue 2 recrop reduite.jpg
07 juin 21 algue 2 recrop reduite.jpg (47.37 KiB) Viewed 942 times
by chrisimbee
Mon Jun 07, 2021 11:59 am
Forum: Pictures and Videos
Topic: alguae
Replies: 1
Views: 945

alguae

Hello, I've just received my photo eyepiece Olympus NKF x3.3 to use with my BH2 :) Indeed the optical quality is much better !!! But the best thing is when I tried it with my Nikon D3100 instead of my astr cam ZWO ASI 120MC that I usually use. The color management is much much better and for once I ...
by chrisimbee
Sat Jun 05, 2021 6:44 am
Forum: Introduce yourself
Topic: Germ-A-Phob
Replies: 9
Views: 4155

Re: Germ-A-Phob

Welcome Cruzer1100 , microscopy is an amazing world full of beauty and knowledge :) If it can make you feel better i was told on another forum that beautifull images had not its place in microscopy and that only information in the image mattered !! So dont worry :) there are all sorts of microscopis...
by chrisimbee
Sat Jun 05, 2021 6:13 am
Forum: Pictures and Videos
Topic: Physarum polycephalum : "blood" circulation
Replies: 2
Views: 1423

Re: Physarum polycephalum : "blood" circulation

Hello Sir, thank you very much in fact I have no protocol and I just put the petri dish without its lid on the stage and used the X10 objective. But you can do the same in bright field . In fact the easiest way would be to make an agar plate in a smal petri dish but with a very thin layer eg 1mm thi...
by chrisimbee
Fri Jun 04, 2021 6:15 pm
Forum: Pictures and Videos
Topic: Physarum polycephalum : "blood" circulation
Replies: 2
Views: 1423

Physarum polycephalum : "blood" circulation

Hello, I have several sample of the "Blob" physarum polycephalum in petri dishes and as i'm preparing sclerotium (dried dormant form) i decided to put some pseudopodes under the microscope. But the thing is that in bright field you dont see much as the creature is quite thick so I thought that maybe...
by chrisimbee
Fri Jun 04, 2021 6:10 pm
Forum: Pictures and Videos
Topic: Lycopodium
Replies: 5
Views: 2153

Re: Lycopodium

beautifull indeed ! :) :o
by chrisimbee
Thu Jun 03, 2021 5:44 pm
Forum: Pictures and Videos
Topic: Hay fusion
Replies: 2
Views: 1165

Re: Hay fusion

very hard to say as the photo is too small and there are no details about the structure etc...
Seen like that it could be colpidium or something else..
by chrisimbee
Thu Jun 03, 2021 5:42 pm
Forum: Pictures and Videos
Topic: Actinosphaerium eichhorni
Replies: 9
Views: 2993

Re: Actinosphaerium eichhorni

really impressive images :) thanks
by chrisimbee
Wed Jun 02, 2021 9:08 am
Forum: Pictures and Videos
Topic: apis mellifera sperm alive
Replies: 2
Views: 1273

Re: apis mellifera sperm alive

02 spermatozoides abeilles acridine.jpg
02 spermatozoides abeilles acridine.jpg (89.79 KiB) Viewed 1246 times

with acridin orange under blue light @ 435nm : we can see the heads that become fluorescent in bright green.
by chrisimbee
Wed Jun 02, 2021 8:45 am
Forum: Pictures and Videos
Topic: Classic cheek cells
Replies: 2
Views: 1114

Re: Classic cheek cells

02 juin 21 cellule de la joue épifluo x100.jpg
02 juin 21 cellule de la joue épifluo x100.jpg (68.53 KiB) Viewed 1112 times
02 juin 21 cellule de la joue épifluo x100 bactéries.jpg
02 juin 21 cellule de la joue épifluo x100 bactéries.jpg (55.67 KiB) Viewed 1112 times
by chrisimbee
Wed Jun 02, 2021 8:23 am
Forum: Pictures and Videos
Topic: Classic cheek cells
Replies: 2
Views: 1114

Classic cheek cells

hi,

I just wanted to try the classic observation of cheek cells with acridin orange under blue light @ 435 nm 3W led
01 juin  cellule de la joue épifluo.jpg
01 juin cellule de la joue épifluo.jpg (50.74 KiB) Viewed 1114 times
02 juin 21 cellule de la joue épifluo n.jpg
02 juin 21 cellule de la joue épifluo n.jpg (44.19 KiB) Viewed 1114 times
The bacteriae become visible and the nucleus is more defined.
Chris
by chrisimbee
Tue Jun 01, 2021 11:35 pm
Forum: Pictures and Videos
Topic: apis mellifera sperm alive
Replies: 2
Views: 1273

apis mellifera sperm alive

Hello, I've bee a beekeeper for more than 15 years now and I thought It would be interestiong to observe honey bee sperm from drones as they are really very motile and active.. Here is a preliminary video to give yu an idea what they look like. https://www.youtube.com/watch?v=nB1Bnlq05wg You got to ...
by chrisimbee
Tue Jun 01, 2021 9:45 am
Forum: Articles and images to be published
Topic: DIY Fluorescence Microscopy
Replies: 8
Views: 12412

Re: DIY Fluorescence Microscopy

as a fluorescent dye I mainly use the orange acridin (you can buy it on ebay at a really good price) as it changes color depending on the pH and if it links to DNA or RNA ; dead or alive matter... It works great with live protozoa and bacteriae for example.. 3W led @ 455nm or 470nm are available on ...
by chrisimbee
Tue Jun 01, 2021 9:42 am
Forum: Articles and images to be published
Topic: DIY Fluorescence Microscopy
Replies: 8
Views: 12412

Re: DIY Fluorescence Microscopy

hi ; I was given a epifluorescence module with my BH2 olympus and inside the module theres is a lens to compensate the extra optical lengh and it augment the overall magnification by a 1.25 factor. I didnt have the lamp house with the module so I bought a second hand one on ebay and replaced the mer...
by chrisimbee
Mon May 31, 2021 7:33 pm
Forum: Pictures and Videos
Topic: small alguae leave under blue light
Replies: 0
Views: 808

small alguae leave under blue light

Hello, just a trial with the new lamp house( for my fluorescent module ) found on ebay.. I replaced the mercury lamp by a 3W led @ 455 nm (royal blue) with a driver bought on amazon.. 24 mai 21 lampe house.jpg 24 mai 21 1er prototype brouillon.jpg 31mai 21 épifluo algue X 20.jpg 31 mai 21 épifluo x4...
by chrisimbee
Sun May 23, 2021 6:18 am
Forum: Pictures and Videos
Topic: Actinosphaerium cell division
Replies: 8
Views: 2660

Re: Actinosphaerium cell division

this is very informative and beautifull !

thanks
by chrisimbee
Mon May 17, 2021 10:07 pm
Forum: Pictures and Videos
Topic: Rainwater squiggly
Replies: 2
Views: 1206

Re: Rainwater squiggly

woaw !! very nice pic !!
by chrisimbee
Mon May 17, 2021 5:23 am
Forum: Pictures and Videos
Topic: onion mitosis : crossover with 2 techniques
Replies: 10
Views: 2951

Re: onion mitosis : crossover with 2 techniques

thank you very much guys :D
by chrisimbee
Sun May 16, 2021 2:06 pm
Forum: Pictures and Videos
Topic: onion mitosis : crossover with 2 techniques
Replies: 10
Views: 2951

Re: onion mitosis : crossover with 2 techniques

i'm going to try again and also try the giant chromosomes of drosophiles and chironomes larvae..