MicrobeHunter.com Microscopy Forum

BTW, I heard if you put a 100W bulb in the Zeiss Axioskop, you risk burning it's power supply. The Light housing is made to support up to 100W, but the Axioskops power supply only supports 50W (I believe there is a 50W labelling in the back of the skop). Some of the "Fluorescence-equipped" Zeiss Axi...

BTW, the picture you are showing is of a Zeiss Axioskop 50 (also sometimes labeled EL-EINSATS, for some reason I'm not to sure about).

Matador

For fun, I also bought these "1pc 10X 22mm / 23mm Wide Filed Stereo Microscope Eyepiece Diopter Adjustable" cheap chinease generic eyepieces just to test and see if Zeiss Axioskop could support 23 mm eyepieces (50 bucks is cheap for a set of two new eyepieces) Link here: https://www.ebay.com/itm/253...

What came standard with the Axio skop 50 (or the Axioskop 20) are the Pl 10x/20 (model 44 40 32) eyepieces (with a 20 mm field of view). (BTW: Axioskop 50 have a 50W external halogen lighting housing system, while the Axioskop 20 has a built-in (under the field diaphragm) 20W light source). The Axio...

Being a pathologist myself, I thought I can share my experience/observations: Nowadays pathology scopes are typically equipped with 2X, 4X, 10X, 20X, 40X and some with an additionnal 1X and 63X objectives as well (the advantage is that the 63X is a dry objective, whearas a 100X is typically an oil i...

Awesome ! Also have an Axioskop 50. I'm in Quebec City, Canada,
My instrument setup : https://www.microbehunter.com/microscop ... =4&t=12711
Welcome to to forum !

Matador

Awesome post. I too am a proud canadian owner of an axioskop 50. My setup here with plenty of pics: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=4&t=12711 I had my farir share of unresolved challenges with adapting this scope. 1) First, I needed an unobtainable part (445265) so I c...

That looks very tempting ... but I must resist ! Being in England, the total cost of purchasing it could be considerable. Although I regret not having one, I’m not really ‘looking for’ an Axioskop. My sign-ff comment says it all really. Many thanks for thinking of me though. MichaelG. True shipping...

No, I regret that I do not have an Axioskop myself ... so this is just an interesting academic exercise for me. MichaelG. I still haven't recieved my order for the lenses but it should be pretty soon. MichealG, If you're still looking for an Axioskop, I came across this one on eBay and it seems lik...

what about an "aromatic" solvent such as xylene or toluene ? I tried n-heptane. Nothing, quick anyway. Heptane is an aliphatic alkane solvant, not aromatic. So while heptane is a very hydrophobic solvant (solvatation by van der walls and hydrophilic interactions), it doee not make pi-stacking inter...

what about an "aromatic" solvent such as xylene or toluene ?

SurplusShed's lens finder is a cheap way to get a variety to try https://www.surplusshed.com/search_lenses.php?type=&diameter_to=22&diameter_from=25&focal_length_to=-55&focal_length_from=-45&sort=&sortby=+asc I just placed an order with them for four lens ranging from 21 to 25 mm diameter with foca...

BramHuntingNematodes wrote: ↑

Mon May 10, 2021 1:41 pm

What was the slide of? Planar corrections are much more noticeable on planar subjects.

Typical histological slide (4-5 micron tissue thickness).

Matador

Interesting. I've read somewhere that non-planar objective should be in focus in 60% of there field of view (FOV) diameter (that is, the center part of the FOV will be in focus), while plan-ojectives should have 100% of their FOV in full focus. The thing is, objectives have a field number (FN). High...

To my understanding, this Auxiliary lens 1.25x (44 52 65) works like this (you rotate the condenser turret to get the auxiliary lens 1.25x in the light path and lower the swing-in/swing-out "front lens" when you need to use the Plan-NEOFLUAR 1.25x objective): Condenser light path.jpg I'm still looki...

MichaelG, thanks for taking the time to explain all this. I have not forgotten you. I actually contacted Ziess to inquire about obtaining this part from them (I was hoping they might have "new old stock", but no luck). They said they do not have part 445265-0000-000 (discontinued). Kind of too busy ...

For that budget, I would personally go for a used infinity corrected scope. For example, I bought a used Zeiss Axioskop 50 on eBay, and then bought Plan-APOCHROMAT and Plan-Neofluar ojectives. Here are some pictures : https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=4&t=12711 I had to ...

I'm curious to know, what scope brand do you have now ? I'm guessing you have a finite (aka fixed tube lenght) system. Most frequent is 160 mm tube lengths. The Zeiss ACHROPLAN 40x/0,65 NA infinity/0,17 and ACHROPLAN 20x/0,45 NA infinity/0,17mm (coverslip thickness) you have in the picture are for i...

...Seems a shame to use up a slot though. Not to mention only 4 slots in the condenser of such a modern microscope... That's a good point. Personally, I don't use darkfield illumination, phase contrast or DIC in my work (I do anatomic pathology and cytology) so it would not matter much to me. But t...

It's not the right lense. I found out that that one on eBay is 1.25 inch diameter wide (too wide for the 24mm slot in the phase condenser turret): https://www.ebay.com/itm/154398048566?_trkparms=aid%3D111001%26algo%3DREC.SEED%26ao%3D1%26asc%3D20160811114145%26meid%3D27d7efa9e9f44712a59e821510dd7759...

Perhaps (???): https://www.ebay.com/itm/194074524813?hash=item2d2fbe3c8d%3Ag%3ADNoAAOSwyKNfwF8x&LH_ItemCondition=1500%7C2000%7C2500%7C3000%7C7000 This is NOT the right lens (NOT compatible with the Axioskop infinity corrected system). I found out that this specific lens on eBay is 1.25 inch (32 mm)...

Perhaps (???): https://www.ebay.com/itm/194074524813?hash=item2d2fbe3c8d%3Ag%3ADNoAAOSwyKNfwF8x&LH_ItemCondition=1500%7C2000%7C2500%7C3000%7C7000 [Picture from eBay seller #1] eBay1.JPG Yes I saw this one, but I'm not sure it's the right kind. The seller does not advertise the lens diameter so I'm ...

Cool video I stumbled on a few years back... Imaging a hidden world (it's a classic):
https://www.youtube.com/watch?v=tPBH0cMf_8s

Better image quality version here: https://www.dailymotion.com/video/x31jvmu

Enjoy

Matador

Here is the complete story in video:

https://www.youtube.com/watch?v=7-LIbAWPc-g

Matador

I'm In Canada and I had success ordering Nye products throught this reseller... Tom Madden TAI Lubricants PO Box 1579 Hockessin, DE 19707 USA (302) 326-0200 (302) 326-0400 fax nyoil@aol.com www.lubekits.com Note that my order was done in 2013. I ordered: 1) 1 x Nyogel 744, 50 gram tube. (29.95$) 2) ...

Also, since resolution also depends on light wavelengs, you get better resolution for the same objective if you use blue light (shorter wavelenght) than if you use red light (higher wavelength).

Matador

I think it's the resolution that is better when the NA is higher.
There are formulas where you can see that resolution depends on NA: https://www.microscopyu.com/tutorials/i ... ion-airyna

Matador

I believe the auxiliary lense (44 52 65) fits in position "1" or "2" (which are the positions that can also accomodate phase ring diaphragms), but not in position "H/DIC" or "DIC" (which are meant to accomodate DIC prisms and also have integrated iris diaphragms). So the auxiliary lens (44 52 65) wo...

Condenser towards nose piece (eBay image): Condenser towards light source (eBay image):

Reading the patent : Yes , I am pretty confident that it is a simple meniscus lens design ... BUT No , I cannot find any reference to the glass or the curvatures Just for the sport of it ... If you can give me a useful dimension for any item clearly visible on the patent drawing: I will try to ‘rev...