MicrobeHunter.com Microscopy Forum

I just found one of Mr. Microbe Hunter Oliver's youtube videos on "conjugate distance/parfocal distance": https://www.youtube.com/watch?v=-4HzdclIW3A I thought I already watched all of his videos twice, but must have missed this one :-) In my work, we say "a little knowledge is a dangerous thing" Ar...

99% of the costumers (us, me included) has no idea what this 185 or 195 stands for, and we buy wrong objectives. Maybe that is a sophisticate fraud scheme to sell more objectives. Ah ha, I think you've help me to decode buying objectives online, thanks! Looking at sellers of these "185" objectives ...

Thanks for the great explanation! Perhaps the parfocal distance should also be part of the markings on each objective? Physically they are easy to tell apart, but when buying an objective online, I have not seen parfocal distance ever mentioned :-) The practical newbie mistake I made with all this i...

Recently I bought a trinocular microscope. All the objectives on this big microscope are physically longer than my small introductory monocular microscope. What puzzles me is both sets of objectives are marked the same, supposedly they are to DIN 160mm standard. Here is a picture of the two 40x obje...

That was an excellent article - read it 3 times and saved for future repeated reference!
Particularly helpful for me was the distinction made between "pure Koehler" and "modified Kohler", which helped to answer my conceptual questions regarding historical and modern day equipment setups. Thanks!

Thought of a few more newbie questions related to the condenser and the lack of a field iris diaphragm on my intro microscope : A) My microscope has a swing out filter holder at the bottom of the condenser, which currently houses what looks like a white diffusion filter. It also has a LED lamp under...

Thanks! Nice to know that I can just leave the condenser raised and not worry about it.

There's no field iris diaphragm on this intro microscope, so no uniform Koehler illumination for me.
I hope I'm using all the microscopy lingo that I'm learning correctly

Thanks again

Hello all, thanks to Mr.Microbehunter Oliver's youtube videos, which got me interested in this as a hobby, I bought an introductory Bresser Biorit TP microscope that has an Abbe condenser. Based on youtube video 001 "How to use a microscope", I follow these steps for using the condenser: 1) start wi...