MicrobeHunter.com Microscopy Forum

The patch is na specific but the aperture width effects the contrast.
A narrow gap of around 1mm will improve the resolution.

Zeiss coated optics cleaner spray.
For when you assume the coating is damaged after isopropyl meths soapy didn't work.

Vintage diatom slides are generally not expensive.
SEM images will help to determine if you are seeing aberrations or resolved structures.

It's a subject covered here.
I'd value a PL/PH trinoc iscope at £500 boxed in as new condition.
LED is good for not boiling live subjects.

I have an iscope pH pl trinocular. The slide clamp is junk. The auto power off isn't true. Apparently the fine focus can work loose but can be fixed. Is it worth the new price? No. It's not Kohler, it's a diffused 3w neo LED with a field iris. The objectives look similar to motic but they are not th...

Low power condensers are the least suited to DF. Dry will be limited to 0.85 realistically but 0.75 is a more likely limit for 1.25. Oil would be for 0.5 to 0.9,lower na objectives will only have the centre illuminated. There are exceptions as always. If you have a phase telescope and a slide ringin...

I've only seen bonded to make a single element filter.

Think you'll find the yellow and blue fringing is how the subject edges are illuminated,the objective can't do a busting lot about it.

But I do notice a small coloration off to one side of field dia. during Kohler alignment. This is more obvious when condenser is oiled which minimizes reflections between condenser and slide. I'll have to examine this later thanks to your post. Thanks, Glenn Likewise, I never tried centration of th...

G4 sockets allow for error in filament placement. Custom bulbs with a collar require a jig to focus the collar fitment precisely. Probably not a good idea to run 6v 30w on a supply intended for 20w. 6v @ 30w is 5 amps. 6v @ 20w is 3.5 amps. Either the voltage will be low and give a warm tint or the ...

Another great contribution thanks. Striving for perfection in production is the biggest waste of time and money resulting in bankruptcy or buy out. There is an agreed standard, sometimes that is exceeded by chance. Below that is usually left for the phase B tester to dispute and resolve. The intende...

Happy days.
We have Watson and V***ers in the UK.
It's a slippery slope if you don't have a lot of space, the Watson microsystem 70 cases are designed to be stacked.
Good luck from sunny Devon.

Optimised for epi illumination?
What 10x do you have?

Excellent, thanks for sharing your find.

More useful for inverted scope.
Would work ok for lower na, maybe 2x.

The light source is the cause. LED systems with pretend Kohler will have similar aberrations. It's just the way things are. The objective can't fix the problem. It could be cost effective to pick up a Labophot 2 with a dead PSU but working fine focus and add a variable 12v 5A DC supply. There are 2 ...

Close and focus on the Kohler iris. There will be coloured edges. LED is the cause not the objective. There are but the colours vary according to the objective. I can almost get rid of the edges when everything is properly aligned, especially with the Plan Apo and Fluor objectives. 100% what I woul...

Close and focus on the Kohler iris.
There will be coloured edges.
LED is the cause not the objective.

If you like yellow and blue fringing LED is an upgrade.

Something is out of alignment.
Wrong bulb or LED "upgrade".
Unlikely to be the objectives.

I'd wonder about the mountant being an issue.

More than anything if the slide clamp is up to holding a slide and the plastic mechanical parts hold up.
There's the yellow and blue fringing from the pretend Kohler and LED to deal with.
The objective can only do so much.

Sticking with 275.

Best avoid anything that looks like it's already lived in marine biology labs.
Good luck with finding a bargain, they are available.

The biggest difference is the cleaning of samples. Residues will quickly settle Infront of the subjects. Settling and rinsing may or may not be interesting. DF is more challenging but what is life without taking some on. You should get a lot for the equivalent budget. I don't use mine much but would...

000 sable brushes are worth trying for moving things with minimal damage.

Definitely a junk pile of projects.
If the labophot 2 PSU is working it may be viable, Labophot 2 complete with failed PSU are cheap and abundant.

I'll kick off with 275 microns ish across the round diatom.

I'm confident you will enjoy the DOF from x40 .65 far more than the resolution at the limits.
X20 .5 is great when you train yourself to see what is there.
Get some bargain diatom strew slides from eBay and just have fun with it.

How long is the lag from posting here to when it appears on their site?