MicrobeHunter.com Microscopy Forum

;) My oil dark field condenser works at 10, 20, 40 and 100x, but the 100x objective has a built in iris. What a difference. Greg Thanks Greg! I appreciate your note. I did manage to make a couple of darkfield stops. They work well, but only at 4x and 10x, not at 40. I think I’ll just have to pick u...

The brand should be able to fit your condenser holder is the biggest concern . There is also the paraboloid v cardioid. The cardioid is more efficient, but more finicky about stuff like centration and slide thickness also more expensive. Unless you are doing a lot of immersion lens work the parabol...

The quality of dark field imaging is dependent on how highly corrected the condenser is. There are various designs of DF condensers but they all have in common the fact that they correct for ca and other aberrations better than an abbe condenser. With higher N.A.'s they are also more precise, so th...

The quality of dark field imaging is dependent on how highly corrected the condenser is. There are various designs of DF condensers but they all have in common the fact that they correct for ca and other aberrations better than an abbe condenser. With higher N.A.'s they are also more precise, so th...

Yeah stops work ok for under the 40x, kind of iffy for an achromat 40x, unusable for a 40x fluorite or Apo or anything of a higher magnification. Also, if you were doing diascopic fluorescence you would want a darkfield condenser to shoot maximum abs. freq onto the sample but get a minimum of it in...

DIY darkfield patches do not work well above NA 0.65 (usually 40x objective, below that patches work just fine). Dedicated darkfield condenser has mirrored internal surface for better light efficiency and can also provide better optical correction. Thank you! Are you able to tell me if the oil DF c...

Hey Folk, I am new to this site and is my first time posting a question. Hello!! Is there a reason why someone would buy a dedicated dark field condenser instead of making their own? I cannot seem to find anything on the web. Please let me know your thoughts Thanks, NReekay In my limited experience...

Generally no, eyepieces in microscopy are differwnt from telescope eyepieces. In some microscopes the eyepiece is an essential part od the optics, correcting for aberrations intentionally left in the objective design to make a fully corrected image. Even outside of that compatibility ia not univers...

BramHuntingNematodes wrote: ↑

Tue Jun 01, 2021 4:37 pm

No don't do that.

Thank you!

Lovely!

Hey Folks,
I was wondering if one should automatically replace the eyepieces/oculars that your microscope came with? Usually when you buy a new telescope, that’s one of the first things you do... but what about with microscopes?
Thanks,
NReekay

Hey Folk,
I am new to this site and is my first time posting a question. Hello!! Is there a reason why someone would buy a dedicated dark field condenser instead of making their own? I cannot seem to find anything on the web.
Please let me know your thoughts
Thanks,
NReekay