MicrobeHunter.com Microscopy Forum

Turn the correction collar and check what happens.

apochronaut wrote: ↑

Mon Apr 15, 2024 11:27 pm

Can I get aluminum toxicity from that water?

Be careful! Aluminium is suspected to cause Alzheimers's disease. That's why drinking canned beer causes strange behavior.

The more nervous amongst us have a tendency to find documents like this one from Zeiss: https://www.fluorescencemicroscopes.com/wp-content/themes/CompoundMicroscopes/pdf/Zeiss%20HBO%20100%20Microscope%20Illuminating%20Operating%20Manual.pdf Even the more nervous amongst us will read in this documen...

I am still using mercury lamps and never had any problem with them. 1. UV light from a mercury lamp isn't more dangerous than UV light from a high-power LED. 2. A technically proper setup does not carry any UV risks. A full working days scattered UV light is much less than one gets from a 10 minutes...

To get dark field with the 100x you need some dedicated dark field condenser.

The L40/0.60 is nothing you want on your DRMB. It is designed to look through the bottom of Petri dishes. The correction collar is from 0 - 2 mm. Imagine the fiddling to set it properly to 0.17 mm.

I wouldn't buy a microscope with objectives from another brand.

Based on what I read of Leitz 600 and 400 series condensers, their intercept distance with the flip-up top is around 1.2mm, please see below. Doesn't this mean that my problems are likely caused by the condenser not reaching a high enough position, close enough to the slide? Please see the photo I ...

A centering disc is easily made DIY. Just print it on semi-transparent paper. The exact dimensions are not critical at all.

The lamp condenser is clearly defective. It must be centered on axis to work properly.

It depends on the definition of "a". There are trillions of microbes in the world. At least one of them will eat at any given time. The answer to your question is "yes" so far. If the intention of your question is, if we always know about any of the trillions of microbes when they are eating, the an...

The direct successor of the Diavert was the DL IM. First under the Leitz brand then Leica. Under Leica brand it made the change from finite to infinite optics. The Labovert was a very special animal standing separately. When using Olympus objective the fitting Olympus eye-pieces will give best resul...

It is time to give up on this. Stay with your believe.

Cropping does not increase frame rates. Cameras doing 1000 fps are sold at prices far beyond everything Touptek has on offer.

And yes, I don't know what I am speaking about.

The quoted specs of your touptek camera say "Ultra-fine Color hardware Color Enginne ensuring high frame rates(Up to 15 frames for 20M Resolution);" 15 frames is a bit away from 1450 frames. Do the math how low you must go with resolution to achieve 1450 fps. And yes, I don't know what I am speaking...

Your camera and its USB connection would not do 100 FPS.

Topcode wrote: ↑

Sat Mar 02, 2024 3:53 pm

Alexander wrote: ↑

Sat Mar 02, 2024 9:57 am

You are confusing exposure time with frame rate.

No? The framerate means that roughly 1450 frames were captured every second, exposure time was about 60 microseconds by my recollection.

How could you expose 1450 times 60 milliseconds in one second?

You are confusing exposure time with frame rate.

This is no Laborlux it is the smaller Leitz SM. The Laborlux had build-in illumination. It is nicely equipped with binoculars, Berek condenser and a good stage but it looks like the mirror is missing. The Heine alone will sell for more than the microscope. The dark-field condenser will sell easily a...

If you had any pictures.

Buy the tips for the maximum capacity, in your case 200 μl.

They look all the same on web pictures but are very different in real size. A 10 μl tip would not fit mechanically on your pipette.

onilink_ wrote: ↑

Wed Feb 21, 2024 6:07 pm

I know some corrections are also made in the tube lens, but I don't think it explains the difference between my 100x/80x and this 40x.

Reconsider that point.

Metallurgical objectives work in transient light as fine as in reflected liegt. The question is: fits the objective the system. There are some M26 to RMS adapters on the market, it should not be a problem. My concern is more on the side of coverslip. Fitting into a system is more than just the thre...

Metallurgical objectives work in transient light as fine as in reflected liegt.

The question is: fits the objective the system.

Maybe this 1inch sensor RisingCam could be an option for the future? https://www.aliexpress.us/item/2251832581795360.html?spm=a2g0o.store_pc_home.hotSpots_2006221742460.0&gatewayAdapt=glo2usa4itemAdapt But from what I learned, the overall picture information/quality may not be much better from the ...

Topcode wrote: ↑

Sun Feb 11, 2024 7:39 pm

source?

Read sources carefully. Did you find any proof that the posted data of IMX 585 was at 400.000 ISO? What are the sensitivity and noise level of the D6 at 100 ISO?

Just do the test. Compare the cameras in real live not in sales literature.

That will depend on your objectives and dye. I set up a friend's lab with a leitz I refurbished with led lamphouses and a dslr for shooting and it gives them at least adequate results for their research. Exactly. But a consumer DSLR is much more sensitive than 99 % of all dedicated microscope camer...

1'' sensor will fit well. Most dedicated microscope cameras are not sensitive enough for fluorescence work and those who are cost a fortune. This is one from the cheaper end of scale: https://de.aliexpress.com/item/32836591245.html?pdp_npi=4%40dis%21EUR%21€%201.273%2C67%21€%201.209%2C99%21%21%211340...

Doesn't matter, just take what is available for your scope.

It is quite some special objective requiring the fitting condenser. The offer you found on Ebay is ridiculous and will never sell.

150 USD is what I personally would pay for it but you may find somebody who is willing to pay more.

I simply use standard Petri dishes with my Diavert, the only exception being fluorescence work using oil immersion objectives.