MicrobeHunter.com Microscopy Forum

Then the questions are: 1. Does these APO require eyepieces to correct ca? 2. Leitz Pv APO is phase contract APO? I've never seen phase contrast APO. How does it perform? 3. What is the parfocal length of Leitz 170mm system? Is it also 45mm? 1. Yes, they require Leitz Periplan eyepieces for full co...

Methylene blue 1 % in water is a strong staining solution. Depending on the use case it can be used pure or diluted further. Eosin depends on the use case: 1 % in water if used as a counterstain to hematoxylin. 1 % in methanol or ethanol if used to stain mitochondria, plasma proteins and collagen. T...

My guess is, they would use very similar immersion oils like us.

As carbon based life forms we use materials containing every element in the periodic systems. Why should a silicon based life form behave different?

MitoTracker Green is reported to stain mitochondria. Your pictures match that.

And yes, acridine orange stains the nucleus.

There are oil immersion objectives from 10x up.

Examples I own and use:

10/0.45 oil
20/0.75 oil
40/1.3 oil

Children born of the Apollo space age with an inclination towards science, as I was, all wanted a microscope, a telescope and a chemistry set. Never wanted a telescope but hat a microscope and a lot of chemistry stuff. Many of the experiments I did, would be considered a criminal offense today. As ...

An educated guess is still a guess. I wouldn't go further without being sure.

Guess you realize, that this is a quite valuable item.

Hi Steve.

"Inko" was used by Zeiss for their early DIC condensers.
The constructions, that allows movement along one axis is another indicator.

I am not perfectly sure and did just a guess.

looks like some DIC prism for reflected light.

A trino for the SM-Pol never existed.

There are bino's but they lack the Bertrand lens and feature a lesser field of view than the mono. Hard to find anyway.

Turn the correction collar and check what happens.

apochronaut wrote: ↑

Mon Apr 15, 2024 11:27 pm

Can I get aluminum toxicity from that water?

Be careful! Aluminium is suspected to cause Alzheimers's disease. That's why drinking canned beer causes strange behavior.

The more nervous amongst us have a tendency to find documents like this one from Zeiss: https://www.fluorescencemicroscopes.com/wp-content/themes/CompoundMicroscopes/pdf/Zeiss%20HBO%20100%20Microscope%20Illuminating%20Operating%20Manual.pdf Even the more nervous amongst us will read in this documen...

I am still using mercury lamps and never had any problem with them. 1. UV light from a mercury lamp isn't more dangerous than UV light from a high-power LED. 2. A technically proper setup does not carry any UV risks. A full working days scattered UV light is much less than one gets from a 10 minutes...

To get dark field with the 100x you need some dedicated dark field condenser.

The L40/0.60 is nothing you want on your DRMB. It is designed to look through the bottom of Petri dishes. The correction collar is from 0 - 2 mm. Imagine the fiddling to set it properly to 0.17 mm.

I wouldn't buy a microscope with objectives from another brand.

Based on what I read of Leitz 600 and 400 series condensers, their intercept distance with the flip-up top is around 1.2mm, please see below. Doesn't this mean that my problems are likely caused by the condenser not reaching a high enough position, close enough to the slide? Please see the photo I ...

A centering disc is easily made DIY. Just print it on semi-transparent paper. The exact dimensions are not critical at all.

The lamp condenser is clearly defective. It must be centered on axis to work properly.

It depends on the definition of "a". There are trillions of microbes in the world. At least one of them will eat at any given time. The answer to your question is "yes" so far. If the intention of your question is, if we always know about any of the trillions of microbes when they are eating, the an...

The direct successor of the Diavert was the DL IM. First under the Leitz brand then Leica. Under Leica brand it made the change from finite to infinite optics. The Labovert was a very special animal standing separately. When using Olympus objective the fitting Olympus eye-pieces will give best resul...

It is time to give up on this. Stay with your believe.

Cropping does not increase frame rates. Cameras doing 1000 fps are sold at prices far beyond everything Touptek has on offer.

And yes, I don't know what I am speaking about.

The quoted specs of your touptek camera say "Ultra-fine Color hardware Color Enginne ensuring high frame rates(Up to 15 frames for 20M Resolution);" 15 frames is a bit away from 1450 frames. Do the math how low you must go with resolution to achieve 1450 fps. And yes, I don't know what I am speaking...

Your camera and its USB connection would not do 100 FPS.

Topcode wrote: ↑

Sat Mar 02, 2024 3:53 pm

Alexander wrote: ↑

Sat Mar 02, 2024 9:57 am

You are confusing exposure time with frame rate.

No? The framerate means that roughly 1450 frames were captured every second, exposure time was about 60 microseconds by my recollection.

How could you expose 1450 times 60 milliseconds in one second?

You are confusing exposure time with frame rate.

This is no Laborlux it is the smaller Leitz SM. The Laborlux had build-in illumination. It is nicely equipped with binoculars, Berek condenser and a good stage but it looks like the mirror is missing. The Heine alone will sell for more than the microscope. The dark-field condenser will sell easily a...

If you had any pictures.

Buy the tips for the maximum capacity, in your case 200 μl.

They look all the same on web pictures but are very different in real size. A 10 μl tip would not fit mechanically on your pipette.