MicrobeHunter.com Microscopy Forum

Thanks for the explanation, it's clear to me now!

Thank you all for your replies. The slide doesn't belong to me but I was fortunate to borrow it. So Optical Insights is a company that made dual-view camera systems. Essentially, a giant box splits the image into two, this is used to excite two types of dye for simultaneous observation, each image o...

Here are test images of illumination uniformity, taken in transmitted and reflected light with the 4x objective (FN26.5) and an EOS-1D X DSLR and NFK 2.5x photo eyepiece. Screenshot 2023-01-15 at 11.08.55.png Transmitted light, Köhler illumination Screenshot 2023-01-15 at 11.13.38.png Intensity prof...

I came across this interesting slide whose manufacturer's logo is a zebra: https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-LtCFCF7/0/f041225b/L/IMG_3971-L.jpg It has a smooth bronze-coloured reflective surface and a pattern of see-through square grids and circles. I put it under my Olympus BHS...

What do you mean by distorted corners? Do you mean pincushion/barrel distortion near the corners? I didn't notice anything with my UIS2 objectives and FN26.5 BH2 eyepieces, but I wasn't looking very hard and I wasn't looking at a grid. Indeed, the mix of UIS2 objectives, BH2 tube lens and BH2 superw...

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-KMLx9FW/0/d1907795/L/olympus%20BHSP%20microscope%20BH2-MA%20vertical%20illuminator%20reflected%20light%20maintenance%20cleaning-L.jpg I have what I think is the AB138900 inner plate as shown in the photo above. But it is just an empty ring, no g...

1. I think this is the green lens, not the red lens. You are looking at it upside down. 2. Ok I understand. But why did the other members of the forum suggest changing the tube lens? Perhaps the tube lens does correct something that is different between BH2 and UIS2 optical systems? 3. What does "fi...

1. I am quite sure my MA2 doesn't have the red arrow lenses. Maybe they belong to the analyzer slider above, or maybe the lenses were removed for some reason before I acquired the part. 2. Why don't you think it's necessary? 3. Ok, but where is it in the diagram? Is it just another name for the tube...

1. Does your diagram show MA2 or RLA? You said ""MA2 epi illuminator" (RLA)" so I'm not sure which one you're talking about. My MA2 doesn't have the lens with the red arrows. It is missing the entire AB119800. I assume my tube lens is the green one. 2. Yes, the tube lens has different corrections fo...

Thanks for the link. In any case I think I figured out where the 180 mm is. If you refer to the diagram above, I assume it's 33.6 + 151.3, minus the 4 mm overlap. So yes, I realise the "reference plane" is just for convenience.

Although the Olympus tube lenses are all supposed to be "f = 180", I'm still confused. For example with this diagram of the SWTLU (numbers in mm): Screenshot 2022-10-03 at 19.59.26.png Where exactly is the "f = 180" in this diagram? There's the number 151.3 mm from the reference plane to the focal p...

It might also be interesting to try a full UIS-infinity-compatible conversion. I don't know much about the polarization analyzer, but I'd assume the tube lens in another f=180 epi head could be removed and then something like a U-TLA tube lens added in place of a trinocular head for direct imaging ...

https://blogs.zeiss.com/microscopy/en/m ... n-germany/

A short article about the Jena facility which started in the 19th century, made my microscope and is now the birthplace of their most advanced confocals and light sheets.

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-LNGjQQv/0/9aad63f6/XL/IMG_2815-XL.jpg Makeshift polarized-light setup with "burger" made of Olympus analyzers, and deployment of my large-calibre "nuclear warhead" objective, the Zeiss Pol Z Plan 2.5x. The top lens of the condenser was unscrewed...

Zeiss Archives has kindly replied that this instrument was delivered to Bennett (a dealer) in New York in 1926. Is it safe to assume it went on a transatlantic cruise instead of flights? Does anyone know anything about Bennett? In 2022 it would depart New York on probably its very first trip to the ...

I reproduce here a discussion about the oil immersion objective, which arose on another thread about immersion oils. First, apochronaut kindly examined a similar objective in his collection: I've had a look at that objective. Has a tiny serial # 9412 on the front housing lip, kind of illuminating a ...

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-t6dBd7J/0/f9595ab6/XL/IMG_2722-XL.jpg https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-nPXs88J/0/592cae84/XL/IMG_2740-XL.jpg The cap on my objective unscrewed easily. It doesn't seem to be plated as it looks similar on both sides. On the in...

Acetone and chloroform both had no visible effect under a 10x loupe.

Thank you apochronaut for the detailed explanation. I will try to examine the objective with a microscope to see if the seal on the front lens is still intact. I will have to check the condenser too as I was planning to oil it as well. I guess I’ll stick with cedar oil as the front lens housing is m...

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-Dsm4FZG/0/005a841e/XL/YC010713-XL.jpg Something on one of the inner lenses of the eyepiece - is this delamination? I can't remove it with isopropanol and lens paper. Here are the very first micrographs, of the most classic slide in my collection...

I've verified physically that the ocular tube is 23.2 mm and the objective threads are RMS. These standard specs make the instrument more powerful and versatile as you can use any of the finite RMS objectives and matching eyepieces, and the tube length is adjustable. For example they fit my Nikon CF...

Thank you for the insights - it’s good to learn some of the principles behind the constitution of oils of varying index. My objective is likely held together by balsam but I can’t be sure. So I think I’ll stick with cedar oil for now, going with the authentic 1920s experience. But I’m still interest...

Thank you very much for the information. Now I can celebrate its centenary. Queen Elizabeth II may also be interested to know that she was only two months old when this microscope was made. I have also written to Zeiss Archives to ask if they have any information from their production and dispatch l...

After some deliberation, I've decided on the official theme music for my microscope: Anton Bruckner · Symphonie IX Wiener Philharmoniker · Herbert von Karajan Großer Saal, Musikverein · 1978 https://youtu.be/6CEiZ7DTVZ8 I will soon take the microscope on its sea trials and shakedown cruise, and emba...

Screenshot 2022-05-03 at 20.55.02.png https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-Q8XPqfr/1/4e81ed09/XL/IMG_2810-XL.jpg https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-cvqDhHB/0/c5807eea/XL/IMG_2811-XL.jpg The condenser is a three-lens aplanatic of NA 1.4 and focal length 10.5 mm. A...

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-STN8N9L/0/5f46640a/XL/IMG_2418-XL.jpg The exquisite “large XY stage E” is rotatable with no brake as far as I can tell. There is no goniometer, which would have been good for measuring angles. I might try designing and attaching one at a later d...

1 Google Applied Precision Immersion Oils 2. Listed you will find Delta Vision Imaging System User's Manual. Click on that. 3. Download Delta Vision Elite User's Maual.pdf. 375 pages, 22.54 mb. It's got to be there. Greg I can't find "Delta Vision Elite User's Maual.pdf" in step 3 above using your ...

These oils were originally meant for the GE DeltaVision OMX structured illumination super-resolution microscope. Here’s a document from them about selecting the right refractive index oil for the sample using their “oil calculator app”.

Applied Precision was acquired by GE Healthcare some time ago, and subsequently GE shut down their microscopy business if I’m not mistaken. I felt that calling them would be a dead end. I felt there was a fair chance that someone on the forum has used these oils before and could perhaps share not on...

https://photos.smugmug.com/Other/n-xC6Rt/Microscopy/i-2p2qv2q/0/191250e8/XL/IMG_2414-XL.jpg I recently took over a set of immersion oils of refractive indices from 1.500 to 1.534 in increments of 0.002, made by Applied Precision. Does anyone have any SDS or information on the composition of these o...