MicrobeHunter.com Microscopy Forum

Most Lumencor light engines (fancy term for LED) can be controlled with MM.
Not cheap, but great product and a great team are behind it.

Thank you all for your replies. The slide doesn't belong to me but I was fortunate to borrow it. So Optical Insights is a company that made dual-view camera systems. Essentially, a giant box splits the image into two, this is used to excite two types of dye for simultaneous observation, each image ...

Javier wrote: ↑

Mon Jan 16, 2023 3:10 pm

Is there a way to check authenticity?

Do you know of fake ones?

I have a UApo N 40x objective that asks for the BFP1 latch, thus officially incompatible with the normal U-DICT sliders. People often claim there is no difference between DICT and DICTS, I will demonstrate the opposite. BFP stands for back focal plane. Most UIS2 objectives have a BFP of either -19.1...

Oh, I know what this is for. Quite esoteric in nature too. You scored here, depending on how much you paid. I would love a slide like this. So Optical Insights is a company that made dual-view camera systems. Essentially, a giant box splits the image into two, this is used to excite two types of dye...

I will be offering a lot of very nice slides from Klaus, perhaps this or next week.

$500 is a lot, if I recall correctly, a new one is $750-$800? The Nikon, Zeiss, and Lomo ones fit BX, not sure about BH but making it work is just a matter of 3D printing a dovetail. Basically, any oil condenser should be less than fussy to adapt. Evident prices have gone through the roof with many ...

Mas, I wouldn't lose sleep over missing the first Ebay condenser. That U-UCD8 was missing its top lens. Those are even more rarely seen available on Ebay and (since they are the part most likely to be damaged) sell for several hundred dollars. Get them new from Olympus they are around $250. Prices ...

There was a pretty fascinating thread where the founder of InFocus joined this forum: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=4&t=11305 The "exaggerated, self-aggrandizing tone" definitely reads more like a advertisement for Sea-Monkeys at the back of a 1970s comic book than ...

Phill Brown wrote: ↑

Thu Jan 05, 2023 3:46 pm

Or vintage slide from eBay?

Watson slide!
So yeah, an antique slide.

https://i.ebayimg.com/images/g/WYsAAOSwhJNfoCtn/s-l300.jpg Screenshot 2023-01-04 122538.jpg I want these 2 stages, thanks. Sell, everything here: https://www.ebay.com.au/usr/danny7271994?_trksid=p2047675.m3561.l2559 Message me here for a discount, I mark up my prices on eBay because of high final v...

Not the best, 60x was hazy due to improper correction collar adjustment, only realised after recording.
Watch in 4k here: https://youtu.be/jBAl_16RxRw

I have the U-DICTH slider which is the U-DICTHR but it accepts a U-ANT, which I also have and can sell. This was a limited production model. This is in my opinion the superior model unless you have BFP1 objectives. I have a U-DIC100HR prism, not the DP you are after, but this could be the push to ge...

Your condenser prism is not even compatible.
Anyway, enjoy experimenting.

nevermind

Those frames are for electrophysiology patch clamp systems, they are designed to minimise vibrations and intended to work with water dipping objectives. Usually, a high power one is coupled with a low power objective for finding the target. They offer a lot of height between the objective at the sta...

If anything I like the "wrong" one better but that may be because of the phase ring in the -19 lens. Which prisms are you using? They both look incompatible, though the DIC effect is solid so I would say it does not matter too much. If none of the prisms are compatible, the comparison is misleading...

I have seen the NexScope one, the other two are new to me, thanks for sharing. My guess is they are sort of modified oil immersion objective where the glass placement is appropriate for the refractive index of water rather than oil, and therefore they are not comparable to what the big 4 offers. I h...

Deconvolution and super-resolution are similar, but different... Thanks for all the information! I have used SVI's Huygens for widefield and DIC (brightfield methods) deconvolution, the results were very good. For just brightfield, on a screen level, sensible sharpening gives comparable results. Go...

Trying out new setup.

That massive LED is wasteful and delivers subpar results. Reusing the optics of the lamphouse is a very good idea, however, it was not designed for such a huge area. I see no active cooling either, such a small lamphouse is going to choke this ludicrously overpowered LED. I wonder if the lack of bri...

The answer is "its complicated", as you've likely already realised from some of the above posts. Most microscope lenses intended to work with coverslips that are #1.5 in thickness. But this assumes that the sample is attached to the coverslip . If your sample is on the slide, or suspended in medium...

There's improvement with a 60x water immersion objective if live cells are your thing, with water above the cover slip (still a bit messy). However (Macro-Cosmos likely knows) that UPlanSApo 60xW objective probably sells well above $5K - and more for the DIC prism. You can likely find a lesser 60x ...

1) Go for the UPlanSapo 60xW and get the pairing prism. Stay away from that 40x. 2) What is your DIC slider? The 40x needs BFP1 position, U-DICT will theoretically yield subpar results. 40x using fluorescence beads, see how bad the periphery is yourself. https://live.staticflickr.com/65535/520558128...

Look up on the difference between objective magnification, eyepieces, and total magnification. If I project the image onto an outdoor theatre screen, it does not make it 100,000x. If you are lazy like me, just strictly use optical magnification. Objective magnification*projection eyepiece*optovar, ...

Forgive me for being outspoken. The magnification of projection eyepiece, is relative to that of 10X20 eyepiece viewport, so your image magnification should be multiplied by 10 times. photo-eyepieces-nfk-image-sizes.gif http://www.alanwood.net/olympus/photo-eyepieces.html#nfk At least on BHS, I gue...

Very attractive image. Was it post-processed to remove halos ? Yeah, background swap and some cleaning since the slide was pretty dirty. Wait a minute..... 200x + image crop, right? No real cropping, the diatom filled the frame diagonally, 43.128mm, 100x objective and 2x projection eyepiece, which ...

Species should be correct.

Heine phase contrast, 200x.

FWIW, the Ebay BX40 with a DIC nosepiece I PM'd you about, sold for under $1K. Someone managed a great scope at a low price - just the DIC nosepiece typically runs around $500 and the DIC nosepiece adapter another $300. With other components, currently available on Ebay etc., a complete BX40 DIC sy...

Scarodactyl wrote: ↑

Mon Nov 14, 2022 10:12 pm

Nikon made ss prisms for the eclipse.

Thanks, I messed up and did not read the graph I had properly.