Saul, can you please share the 3d file? The output image is very interesting. I would like to try it as well. Appreciate it.Saul wrote: ↑Wed Apr 24, 2024 12:59 amMy DIY version:
https://www.photomacrography.net/forum/ ... 45#p296945
Search found 102 matches
- Thu May 09, 2024 7:30 pm
- Forum: Illumination Techniques
- Topic: The Lucigen illuminator
- Replies: 7
- Views: 734
Re: The Lucigen illuminator
- Wed Mar 01, 2023 12:07 am
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Tue Feb 28, 2023 5:37 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
It doesn't matter where the modulator is located though, as long as it's location is matched by the slit location relative to the condenser. A similar principle is used in locating the phase annulus outside the objective in some phase systems, such as Reichert did also around 1972 and for all contr...
- Mon Feb 27, 2023 11:55 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
That's starting to look right! Nice job. The analyzer slit needs to be at the back focal plane of the objective. For low power objectives, your insert is probably at close to the right place - the back of the objective. For higher power objectives the back focal plane seems to be in the barrel - ha...
- Mon Feb 27, 2023 11:44 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
Hi John,
I recreated my account today and uploaded the files. Looks like I have to wait for 24 hours before I can publish it. Will post the final link around this time tomorrow.
- Mon Feb 27, 2023 11:45 am
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
Sure. Will post it later today.
- Sun Feb 26, 2023 10:16 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
Got hold of ND Filter (12.5% light transmission, close to 15% mentioned everywhere). Changed my approach a bit. Instead of creating a slider as I tried before, I created an insert that goes into the objective backside. It gives better control. Tried it with various combinations ( 10% or 15% dark and...
- Tue Feb 14, 2023 11:13 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
Verified with phase telescope to make sure only the transparent area is covered by nose polarizer. It goes full extinction and create dark background at the highest turn. So it seems 3rd polarizer will not work. I will look for a gray/ND film. BTW, even though it didn't provide desired result(whatev...
- Tue Feb 14, 2023 9:53 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
Ldflan, probably I didnt explain it clear. I didn't change the condenser slit after I set it. I am rotating the light source polarizer after everything is set. What I am moving is the 2 sheets in the nose to adjust the position to put them in place. I followed the information in the user guide below...
- Tue Feb 14, 2023 7:18 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
Improvised something to do the diy HMC quickly. Here is the first version. All the videos were unprocessed and taken quickly. The photos were from the videos a d unprocessed 1. Top one - goes on top of light source. 2. Middle one - filter holder. Slit is for 40x 3. 2x poster board papers in the nose...
- Mon Feb 13, 2023 4:23 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
- Mon Feb 13, 2023 1:17 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
That is the information I was looking for. Thanks.
- Sun Feb 12, 2023 9:31 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
Thanks ldflan for taking time to post the images. It gives me some ideas. I am trying various lighting methods just for fun as I like the challenge. Gives me satisfaction at the end. I thought HMS seems simple enough to diy. :) . Posting sample videos help others make a decision to do diy ( just lik...
- Sun Feb 12, 2023 8:03 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
Perfect. Thanks. Appreciate the response.
- Sun Feb 12, 2023 7:28 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
I doubt that 15% transmission rating for the polarizing film is an absolute must. I would give it a try with whatever linear polarization film you can get. The 1% transmission area of the Hoffman slit (marked on the website you reference as "D") can be omitted, at least at the objective rear. I kno...
- Sun Feb 12, 2023 7:21 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
One more video on the original VAC. Just to avoid confusion, I used one linear polarizer at the light source and the VAC patch mentioned in the link in the first post. The patch goes to filter tray. Then the polarizer at the light source is turned to produce variable effects. Camera couldn't keep up...
- Sun Feb 12, 2023 6:34 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
I see! You are combining circular and linear polarization... at one edge fairly high up in the optical train. I have no clear idea what that is doing... I am getting the gray and blue hue with just 2 proper linear polarizer( when I rotate the bottom one) without the nose filter. It only comes if th...
- Sun Feb 12, 2023 5:49 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
I think you must be mistaken about the 3/4 perimeter not varying. Just rotate the condenser and light source filters against one another and you will see what I mean. The area that is cut out of the condenser filter cannot vary in intensity because only one polarizer affects the light that goes thr...
- Sun Feb 12, 2023 5:37 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
This is a cool thread, and thanks for uploading your video tests; that's a lot of work. Sansub, are you using an inverted microscope? What's your set-up look like for those videos? Thanks :) . Nothing much to do outside in the weekend, so trying things that I kept thinking of doing for sometime now...
- Sun Feb 12, 2023 4:10 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
Thanks for posting the videos. To my eye, the VAC2 setup seems to be giving a much better result than the modified system; the modified system's results seem very similar to normal oblique lighting. When you rotate the light source polarizer on the modified system, it pretty much seems to dim acros...
- Sun Feb 12, 2023 4:00 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
No, the cut out area of the main filter is not the variable area of light transmission. The area of variable light transmission is the 3/4 disk polarizing filter. The 3/4 perimeter can be tuned to extinction using the light source polarizer. Neither of these are Hoffman interference contrast. To ge...
- Sun Feb 12, 2023 2:50 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
As I understand it, the Strange VAC system creates a horizon of variable intensity illumination along 270 degrees of the circumference of the field of view, the result being similar to darkfield (or near darkfield) perimeter lighting condition, but with an oblique lighting effect in which the brigh...
- Sun Feb 12, 2023 2:20 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Re: Simplified Variable amplitude contrast (VAC) setup
Ldflan, Thanks for the detailed response. As you mentioned, yes, VAC seems to be fancy oblique. Atleast that is what I am getting as results. It varies the background light intensity uniformly to get better obliqueness. I didn't have better sample to demo it and make it "uuhh-wow" :D . That is why I...
- Sat Feb 11, 2023 4:39 pm
- Forum: Home-made microscope adaptations
- Topic: Simplified Variable amplitude contrast (VAC) setup & DIY HMC
- Replies: 43
- Views: 10260
Simplified Variable amplitude contrast (VAC) setup & DIY HMC
While searching on the forum, I found VAC setup mentioned and how it is good on certain conditions. While doing further searching, found one earlier thread talking about how to create diy for VAC (link below). Even though you can get that done with a bit of tinkering, I thought I can make it simpler...
- Mon Jan 23, 2023 11:46 pm
- Forum: Pictures and Videos
- Topic: Chilling out with a Paramecium !
- Replies: 6
- Views: 1234
Re: Chilling out with a Paramecium !
Pretty nice video. It is more impressive when I saw your spec. I agree with the phone recording. My microscopes are trinocular. I did setup entry level sony mirrorless camera ( bought it for 250$ used) and spent another 50$ for other accessories but at the end I found out that my phone camera takes ...
- Sat Jan 07, 2023 8:59 pm
- Forum: Illumination Techniques
- Topic: variable oblique filter
- Replies: 7
- Views: 2319
Re: variable oblique filter
Looks nice. Combine them together or alone in oblique manner produces nice effect. I have multi color films as well and playing with it :). They produce nice image up to 20x. 40x is not good in my experiments. https://live.staticflickr.com/65535/52613147071_96d5d0a0b1.jpg 20230107_134545 by San Sub ...
- Sat Jan 07, 2023 2:04 am
- Forum: Illumination Techniques
- Topic: variable oblique filter
- Replies: 7
- Views: 2319
Re: variable oblique filter
Legion air, I took your idea a bit further. I realized that this can be expanded to produce the dic effect mentioned in the below link. http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artnov02/diydic.html Here is the normal oblique slider. https://live.staticfli...
- Tue Jan 03, 2023 4:50 am
- Forum: Illumination Techniques
- Topic: variable oblique filter
- Replies: 7
- Views: 2319
Re: variable oblique filter
Thanks for sharing it. Simple and functional.
- Thu Dec 01, 2022 12:38 am
- Forum: Microscopes and optics
- Topic: Need help choosing objectives
- Replies: 24
- Views: 5324
Re: Need help choosing objectives
Thanks for the response Pete. Sorry, forgot to mention earlier that I do have a generic 20 plan acro objective. I compared the image quality of the phase objectives to the acromat plain from Amscope. The normal acromat image and videos are more crisp. The different is noticeable. Please see the com...
- Tue Nov 22, 2022 6:41 pm
- Forum: Microscopes and optics
- Topic: Need help choosing objectives
- Replies: 24
- Views: 5324
Re: Need help choosing objectives
Hi VCR, I just noticed your message. Yes I did took videos. I didn't use the photo port though. I use my phone camera ( Samsung s21 ultra) which has better quality camera than the cheapo Usb cameras out in the market. It is easier to use as well. I have a 3d printer. So made the "sleeve" for the pho...