Search found 130 matches

by Microscopy_is_fun
Tue Jul 26, 2022 6:41 am
Forum: My microscope
Topic: Home built laser scanning confocal microscope
Replies: 31
Views: 6747

Re: Home built laser scanning confocal microscope

Now, i am focusing on scanning head modeling. After the system finished, i will test the confocal imaging quality between different photomultiplier tubes, and try to use SiPM module designed by Vincent et al. as detector. Very interesting! Here are some more links that might be relevant for the pro...
by Microscopy_is_fun
Mon Jul 25, 2022 8:18 pm
Forum: My microscope
Topic: Home built laser scanning confocal microscope
Replies: 31
Views: 6747

Re: Home built laser scanning confocal microscope

The commercial confocal microscope is tooooooooo expensive for personal use, so, i try to make it in low cost using used parts.(Just for prototype~) :D :D :D Do you already have an idea which galvoscanner and which photomultiplier tube you would like to use? If you haven't seen yet, Thorlabs offers...
by Microscopy_is_fun
Sat Jul 23, 2022 8:14 pm
Forum: Pictures and Videos
Topic: -
Replies: 5
Views: 1317

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by Microscopy_is_fun
Sat Jul 23, 2022 8:22 am
Forum: My microscope
Topic: Home built laser scanning confocal microscope
Replies: 31
Views: 6747

Re: Home built laser scanning confocal microscope

Next, i want to design my own scanning head, and hope better than this. :D :D :D I would be glad to hear more about that when you progress with this project! I used custom LabView programm to control the system. The objectives is olympus PLN20X, the tube is removed from olymupus cx21 binocular obse...
by Microscopy_is_fun
Sat Jul 23, 2022 8:19 am
Forum: About the website, the magazine and this forum
Topic: Spammers proliferate
Replies: 37
Views: 14602

Re: Spammers proliferate

They are taking a break and counting shoes. Seems there are plenty of shoes left, and spammers take over the forum once more. This is a nuisance for the users as well as for the administrator of the forum. I do not understand why the offer of long-standing contributors to help solving this problem ...
by Microscopy_is_fun
Fri Jul 22, 2022 7:43 pm
Forum: Microscopes and optics
Topic: Olympus and Leica
Replies: 7
Views: 1609

Re: Olympus and Leica

I'm thinking of two possibilities: Olympus BX50 or Leica DMLB. Both are of much more recent vintage than an Orthoplan or a Universal and both are upgradeable to PC and even DIC. Anyone have advice on the relative merits of these two systems? I use a Leica DM 2000, which is quite similar to the DMLB...
by Microscopy_is_fun
Fri Jul 22, 2022 7:11 pm
Forum: My microscope
Topic: Home built laser scanning confocal microscope
Replies: 31
Views: 6747

Re: Home built laser scanning confocal microscope

Great work, thanks for showing!

Can you give some more details on the software you used for controlling the scanner and on the utilized objectives and tube as well as scanning lenses?
by Microscopy_is_fun
Fri Jul 22, 2022 7:08 pm
Forum: Pictures and Videos
Topic: -
Replies: 5
Views: 1317

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by Microscopy_is_fun
Thu Jul 21, 2022 8:00 pm
Forum: Pictures and Videos
Topic: -
Replies: 5
Views: 1317

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by Microscopy_is_fun
Thu Jul 21, 2022 7:43 pm
Forum: Pictures and Videos
Topic: -
Replies: 5
Views: 1317

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by Microscopy_is_fun
Thu Jul 21, 2022 7:06 pm
Forum: Pictures and Videos
Topic: The Sport of Microscopy
Replies: 11
Views: 1862

Re: The Sport of Microscopy

Now it works, thanks!
by Microscopy_is_fun
Thu Jul 21, 2022 6:52 pm
Forum: Pictures and Videos
Topic: The Sport of Microscopy
Replies: 11
Views: 1862

Re: The Sport of Microscopy

Hi Don,

unfortunately the videos are set "private", therefore I cannot watch them.
by Microscopy_is_fun
Mon Jul 18, 2022 7:30 am
Forum: Camera systems and imaging
Topic: DSLR vs dedicated microscope camera ?
Replies: 13
Views: 2968

Re: DSLR vs dedicated microscope camera ?

I agree with everything that has been said above. My personal opinion: I would only use a DSLR if you already own it. If you buy a camera, I would prefer mirrorless in case your focus is on obtaining colorful and crisp photomicrographs. If you focus is on documenting and image processing (measuring ...
by Microscopy_is_fun
Fri Jul 08, 2022 6:45 pm
Forum: Home-made microscope adaptations
Topic: Converting an oil immersion objective to a water immersion objective.
Replies: 17
Views: 3902

Re: Converting an oil immersion objective to a water immersion objective.

apochronaut wrote:
Thu Jul 07, 2022 1:04 am
5) An alternate set of shims waiting to be installed.
Great work! How do you decide, which shim thickness you need for converting from oil to water immersion?
by Microscopy_is_fun
Sun Jul 03, 2022 6:47 am
Forum: Camera systems and imaging
Topic: Mirrorless camera and adapter combination?
Replies: 26
Views: 4696

Re: Mirrorless camera and adapter combination?

Your setup looks very good! In addition to the 0,5x lens, many manufacturers also provide 0,63x lenses. But in your case, the setup without any lens seems to work very well, why change it? Overall you have a very compact and usable system, congratulation! The E-M1 camera will deliver high-quality im...
by Microscopy_is_fun
Tue Jun 28, 2022 7:21 pm
Forum: Pictures and Videos
Topic: Small and beautiful freshwater snails
Replies: 3
Views: 828

Re: Small and beautiful freshwater snails

Very well captured scene!
by Microscopy_is_fun
Mon Jun 27, 2022 6:44 pm
Forum: Camera systems and imaging
Topic: Mirrorless camera and adapter combination?
Replies: 26
Views: 4696

Re: Mirrorless camera and adapter combination?

So doing the math and assuming that 12mp is far more than enough while using 10x or 40x objective on my microscopy...and with mainly wanting to do live viewing on a computer with some pictures taken and video recorded in HD. Would there be any advantage of getting something like: Olympus OM-D E-M1 ...
by Microscopy_is_fun
Mon Jun 27, 2022 11:55 am
Forum: Camera systems and imaging
Topic: Mirrorless camera and adapter combination?
Replies: 26
Views: 4696

Re: Mirrorless camera and adapter combination?

I can mount the camera body (without lens) directly to the C mount if I have the correct adapter piece, correct? Yes, that's correct. This is one of the big benefits when using infinity optics: The objective projects an image to infinity. The tube lens corrects (almost) all lens errors of the objec...
by Microscopy_is_fun
Sun Jun 26, 2022 9:53 pm
Forum: Camera systems and imaging
Topic: Mirrorless camera and adapter combination?
Replies: 26
Views: 4696

Re: Mirrorless camera and adapter combination?

Hi cwilli, first welcome to the forum! Your microscope is a setup with infinity optics, which makes camera adaptations rather simple and straightforward. It's certainly better to attach the camera to the trinocular port. From the manual it seems that your trinocular head is equipped with a c-mount, ...
by Microscopy_is_fun
Sun Jun 26, 2022 11:38 am
Forum: Specimens, samples and slides
Topic: Who's Using Fluorescent Markers and Tags?
Replies: 7
Views: 2224

Re: Who's Using Fluorescent Markers and Tags?

There are many textbooks about fluorescence microscopy, but none of them targets "idiots". This is certainly true, since fluorescence microscopy requires quite some technical skills, and also caution when dealing with the frequently toxic and/or carcinogenic fluorophores. The recently published boo...
by Microscopy_is_fun
Sat Jun 11, 2022 5:39 pm
Forum: Pictures and Videos
Topic: Meadow sage under the microscope
Replies: 6
Views: 1418

Re: Meadow sage under the microscope

@Louise, Hobbyst46, FatBassPlayer Alexander: thanks for your encouraging feedback! The stem actually turned out quite well. I also had a look at the leaves, searching for the glandular hairs and the trichomes. Making thin (<20µm) section sof the leaves turned out to be a difficult task. But finally ...
by Microscopy_is_fun
Fri Jun 10, 2022 9:16 pm
Forum: Pictures and Videos
Topic: Meadow sage under the microscope
Replies: 6
Views: 1418

Re: Meadow sage under the microscope

I used the stem of one plant to watch it under the microscope. For this purpose, I fixed the stem in a mix of ethanol, formalin and acetic acid (AFA). Afer 24hrs, the AFA was replaced by 70% ethanol, followed by embedding in PEG (12hrs in water/20%PEG, 12hrs in water/50% PEG, and finally 12hrs in pu...
by Microscopy_is_fun
Fri Jun 10, 2022 9:06 pm
Forum: Pictures and Videos
Topic: Meadow sage under the microscope
Replies: 6
Views: 1418

Meadow sage under the microscope

The meadow sage ( Salvia pratensis ) is a pretty common plant in Europe, Asia and northern Africa. It likes sunny, non-fertilized meadows and typically blooms from end of May into June. As can be seen from Fig. 1, the plant can be quite abundant in some places. The picture was taken in the south of ...
by Microscopy_is_fun
Sun Jun 05, 2022 8:04 pm
Forum: Pictures and Videos
Topic: First Lacrymaria!
Replies: 10
Views: 1918

Re: First Lacrymaria!

Great, your music gives each of those little critetrs a distinct personality!
by Microscopy_is_fun
Sun Jun 05, 2022 6:02 am
Forum: Pictures and Videos
Topic: First Lacrymaria!
Replies: 10
Views: 1918

Re: First Lacrymaria!

Hi Javier,

very cool video, I like especially the parts with oblique illumination.

I was just misisng your amazing self-composed music, which you had in some of your other videos.
by Microscopy_is_fun
Sat May 28, 2022 4:51 pm
Forum: Pictures and Videos
Topic: Another Amoeba
Replies: 2
Views: 740

Re: Another Amoeba

Nice video, well observed! I always find it amazing, how these simple organisms are able to show quite some dedication when they want to reach their goals.
by Microscopy_is_fun
Mon May 09, 2022 9:09 pm
Forum: Pictures and Videos
Topic: Micro Music 15: Chromatic Hunt
Replies: 6
Views: 1315

Re: Micro Music 15: Chromatic Hunt

Thanks for the feedback! If I have something to complain, it is the missing scale bar. Without that, I never know how large these animals are. Is that possible to achieve when doing afocal imaging and using digital zoom to avoid vignetting? Hi Javier, I checked with Davinci Resolve: If you use a sc...
by Microscopy_is_fun
Sun May 08, 2022 9:07 pm
Forum: Pictures and Videos
Topic: Micro Music 15: Chromatic Hunt
Replies: 6
Views: 1315

Re: Micro Music 15: Chromatic Hunt

Hi Javier, very nice video with perfectly fitting music! It really looks like the Dileptus is dancing to your music. I think the colours are fully ok, in darkfield the colours are frequently differing from brightfield. If I have something to complain, it is the missing scale bar. Without that, I nev...
by Microscopy_is_fun
Sun May 08, 2022 8:01 am
Forum: My microscope
Topic: Leica Fan here
Replies: 16
Views: 3471

Re: Leica Fan here

Absolutely, you nailed it!!! A hobby within a hobby. That's my next: https://www.leica-microsystems.com/objectivefinder/detail/objective/506414/ But not for the list price, that's part of the trophy hunting experience, getting it for a good price. You have already a very professional line-up of obj...
by Microscopy_is_fun
Sat May 07, 2022 7:55 pm
Forum: My microscope
Topic: Leica Fan here
Replies: 16
Views: 3471

Re: Leica Fan here

For the 100X oil, I might explore that avenue in the future. But do note that, in order to have the best working environment for that objective, you'd have to also replace the dry condenser with an oil condenser. Then I'd want DIC for that, too, that would require a K4 condenser prism and a DIC pri...