MicrobeHunter.com Microscopy Forum

I can do blue excitation light. But i would like to see the red autoflorescence of vegetal stuff. I use a RGB LED torch that works wonderfully but i have two diferent position for the cube filter and i think that none of them allows for that. Should i try to change the filters in the cube? I think b...

I see you some of you have lots of microscopes. I really think i am fine with 3.

I could save more space for a electron microscope.... XD

I was on my parents House when my dad showed me a toy microscope, now i have a zoom stereo, a kyowa with phase contrast and homemade polarization (with homemade waveplate) and an american optical that i have been cleaning with a fluorescence module that i power with a torch chinese led. I Will put ...

I was on my parents House when my dad showed me a toy microscope, now i have a zoom stereo, a kyowa with phase contrast and homemade polarization (with homemade waveplate) and an american optical that i have been cleaning with a fluorescence module that i power with a torch chinese led. I Will put a...

Well, i have a working fluorescence microscope. An old model without the Mercury lamp. I just put a blue/Green led torch in the tube, select a position for the built-in filters and... It just works. It was a cheap opportunity i couldnt let go. I have to do some minor manteinance. But... Fluorescent ...

That is very expensive for a metal tuve, the microscope is cheap. I will try something else, homemade tube.
Thanks anyway

I have a new microscope coming to home.. (i understand, now, why some of you have a collection of microscopes...)

The trinocular head does not have the original aluminium tube, so i think that some DIY is required.

Any ideas? I would like to put a camera there in the future.

Is there any way to repair a dicroic mirror?

Is the Air bubbler required for the Life to survive?

Would like to hear more about your ecospheres

Hey guys, i am starting to get tired of watching bacteria. I like to use pond water but i my samples dont have a lot of variety. I would like to know what do you do to find new specimens and materials to explore. Maybe should i take some leaves and grass and mixing them with water so i can see some ...

I did It. Using yellow filter from my PC kit with a blue led. I watched some autoflorescence in some leaves. It was not a complete black background but you can tell where is the fluorescent stuff coming. I cant expect proffesional quality or lots of thing yo see because its not a proffesional fluore...

I dont want to use UV, too risky. So with a blue led still not enought.. i think i Will stay with bright fiel, darkfield, polarization, waveplate) and PContrast...

Hey guys, i hope you are doing fine.

Do you think a blue led light and a cheap yellow filter in a standart microscope is enought for seeing some autoflorescence in the samples? Wáter ponds or vegetal tissue... Or i should forget that?

For a hobbyist UV excitation is of limited use anyway. It is widely overrated by many. In most cases you are better off with blue excitation. Acridine orange as a stain is very versatile, cheap and easily available while stains for UV excitation tend to be very, very expensive and are rarely availa...

One reason that trans-fluorescence has been phased out is, as commented above, its inability to fully separate the excitation from the fluorescence - the major challenge of basic fluorescence inspection and measurements.. Trans-fluorescence has been practiced with darkfield condensers; those provid...

In a first step differentiate between transmitted light and epi-fluorescence. Transmitted light: You need some excitation filter and an analyzer filter. The first under and the second over the specimen. Very much the same as polarization. The light source must deliver the needed wave length. This t...

I have seen a few videos and tutorials on the internet about homemade Fluorescence microscopy.

Is there any info or experience in this forum?
Thank you!!

Well i sanded and polished a little the new condenser and it fits so i have my first phase contrast view!! I may try to convert my 60x to PC so i can use the same condenser annulus. Respecting the multiuse turret... My dad is going to help me with that, i will upload some photos of the proyect, but ...

Well, i have a phase contrast condenser and an objective and they are designed to work together. But i received it today and the condenser does not fit into my microscope. The phase contrast condenser is maybe 0.5 mm larger than my regular brigth field condenser. So close but... i can sand the new c...

Yes, i think you both are rigth. Thing is the price is too much for something i can already do.
My microscope is a humble kyowa unilux 12 and a condenser turret will cost more than half of it.

Hey guys, i am wondering if a homemade rotating condenser plate is possible. I mean, i use brigth field, dark field, oblique field, polarization (with some non professional waveplates).. and i have recently ordered a 40x phase contrast objetive with a PC condenser. And i know i have to change the ir...