Search found 12 matches

by AquaLifefu
Fri Apr 15, 2022 3:49 am
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

Dishsoap and lemon water as Deparaffinizing.

https://www.ncbi.nlm.nih.gov/pmc/articl ... ort=reader

This seem to be easier to do and less clean up compare to the oil.
by AquaLifefu
Wed Apr 06, 2022 8:24 pm
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

I'm currently trying it out with my spider sample. Please let us know, how your "fried spider" finally turned out! Apparently hempsteadhalide send me hoyer instead of eurpral, i noticed that too late so not sure how it gonns turn out. My oven lowest is 170f so end up doing that, my hot water only d...
by AquaLifefu
Mon Apr 04, 2022 10:37 pm
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

Before staining, the sections were dewaxed in pre-warmed bleached palm oil at 60C for 15 minutes. The slides were stood upright for 1 minute to drain off excess oil and rinsed in 2 changes of 1.7% dish washing solution pre-warmed at 60C for 10 minutes each to degrease the sections before rinsing in...
by AquaLifefu
Mon Apr 04, 2022 9:41 pm
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

I'm only putting up this discussion for those that would prefer a safer alternative and to see if anyone have any input in this since I have not seen any thread about this technique . Hi AquaLifefu, first thanks for all your research on this topic and your input! Actually I would be happy to replac...
by AquaLifefu
Mon Apr 04, 2022 6:46 pm
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4668515/ Bio-Friendly Alternatives for Xylene – Carrot oil, Olive oil, Pine oil, Rose oil From this research, the use of these oil cause less shrinkage, while pine oil actually show better results than xylene, they were place in hot air oven at 140F for ...
by AquaLifefu
Mon Apr 04, 2022 9:39 am
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

Rather than those fancy vegetable oils I recommend the use methyl benzoate instead of xylene. One of its advantages is its tolerance against traces of water. It is not poisonous but more expensive than xylene. When it comes to the "horrible" danger xylene represents, please remember that your mount...
by AquaLifefu
Mon Apr 04, 2022 8:46 am
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Re: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

Both palm kernel oil and coconut oil solidifies at room temperature and require an incubator to keep it liquid for clearing, so the most convenient oil to use would be ground nut oil(peanut oil) from what I understand . https://www.srmjrds.in/article.asp?issn=0976-433X;year=2019;volume=10;issue=4;sp...
by AquaLifefu
Mon Apr 04, 2022 8:04 am
Forum: Specimens, samples and slides
Topic: Coconut oil(vegetables oil) as clearing agent alternative to Xylene.
Replies: 22
Views: 7489

Coconut oil(vegetables oil) as clearing agent alternative to Xylene.

I'm currently in the process of making a permanent mount and found a video in the community that said to use xylene as clearing agent. I was told it was toxic but didn't think it was that toxic until I done research about it "toxic include acute neurotoxicity, cardiac and kidney injury, cancer, bloo...
by AquaLifefu
Fri Mar 25, 2022 8:27 pm
Forum: Microscopes and optics
Topic: Is this lomo plan apo objective for 160 or infinity?
Replies: 11
Views: 2934

Re: Is this lomo plan apo objective for 160 or infinity?

apochronaut wrote:
Thu Mar 24, 2022 8:11 pm
It is usually on the other side.
The back of the objective only said lomo and the serial number.
Image

Also, the quality is better bad like you said compare to the standard achromatic.
by AquaLifefu
Thu Mar 24, 2022 2:29 am
Forum: Microscopes and optics
Topic: Is this lomo plan apo objective for 160 or infinity?
Replies: 11
Views: 2934

Re: Is this lomo plan apo objective for 160 or infinity?

The rule of thumb on Lomo is that the objectives marked with a focal length are infinity corrected. If you go to ebay and have a look at the listings from rafcamera , he usually has a comprehensive breakdown of the specs. of his objectives including the tube length correction. I see that the object...
by AquaLifefu
Wed Mar 23, 2022 8:43 pm
Forum: Microscopes and optics
Topic: Is this lomo plan apo objective for 160 or infinity?
Replies: 11
Views: 2934

Re: Is this lomo plan apo objective for 160 or infinity?

MicroBob wrote:
Wed Mar 23, 2022 8:39 pm
Hi,
do you know Wolfgangs LOMO-Infothek: http://www.mikroskopfreunde-nordhessen.de/lomo.php
You can find there the probably biggest archive abbout russian microscopes, it is likely that there is a document which describes your objective.

Bob
Thanks, I take a look at it.
by AquaLifefu
Wed Mar 23, 2022 8:30 pm
Forum: Microscopes and optics
Topic: Is this lomo plan apo objective for 160 or infinity?
Replies: 11
Views: 2934

Is this lomo plan apo objective for 160 or infinity?

I was wondering if this Lomo plan apo is for 160 or infinity, I thought I got it right until I noticed the F when I received them, I even got compensating K7 and K10 too so if it not for 160, this is pretty disappointing. If it is not for 160, any idea what I can do with these two pair of compensati...