MicrobeHunter.com Microscopy Forum

Thanks all. I suppose each image took between 1 and 2 hours, for taking it and processing it.

405nm or 45nm light Firstly, congratulations on the beautiful images Secondly, is 45nm written correctly? Which image? Many thanks. You know, I went through and read and reread that a couple of times, and still missed that I had written 45nm. I've corrected it now thank you - the images were done w...

Superb. I especially like the Surirella and Stauroneis. Thanks. They have all taken a bit of a hit on resolution compared to the originals (resolution reduced for sharing in the forum). The originals were almost full frame on a Nikon d800 (about 35Mp) and that has impacted the image quality a bit, ...

A few diatom images from a range of Brunel test slides. These are transmission images, done using 405nm or 450nm light, with oblique light (Olympus Aplanat Achromat condenser) and with a 60x Olympus Splan Apo NA 1.4 oil immersion objective. Microscope is a modified Olympus BHB and the camera a monoc...

I'll come back to this thread with a bit more info when I have more time (currently laid up with Covid and spending 20 hours a day sleeping). I built a uv transmission microscope and eye safety was a key concern. I use filters below the eyepieves which block >99.99% of the light below 400nm. 97% doe...

That is a really interesting way to get DF. Excellent results too. Since that is an objective made for a non covered sample, was it covered? Yes this had a 0.35mm thick fused silica coverglass. As you say it's an interesting approach to get darkfield. I do have some other reflecting objectives (mai...

Thanks all. Chas, in theory yes, but I would imagine it would need very long exposure times (due to the amount of light actually getting through). Cyanotype should be most responsive to UV below about 380nm (https://pubs.rsc.org/en/content/articlelanding/2014/pp/c4pp00166d) so a 365nm LED should be ...

This was done on my UV modified Olympus BHB. Darkfield image of a diatom at 313nm. Condenser was an antique Zeiss quartz one, stopped right down (normal condenser, not a darkfield one). Objective was a 15x Edmund Optics NA 0.28 reflecting objective. I guess because the condenser was stopped right do...

Beautiful images, JMC !! The properties of titanium oxide are definitely tempting to use as high RI medium, however it will only work in a film form or a suspension of nano particles, both of which are not really realistic under home microscopy... Thanks. Yes, these metal oxide or metal layers cert...

Amazing contrast and dotting power. Almost like SEM. Oblique light stacking, I am guessing? Offset oblique or circular oblique light, with or without polarization? Thank you, JMC. Thanks. No polarisation. Just bright field, but my Olympus Aplanat can be pulled to one side which was done here (I gue...

In a desperate attempt to stave off work for a few minutes, I thought I would share some images from some slides I recently received from the Postal Microscopical Society. These are Aluminium coated ones by John Dale (who has been mentioned above). These were imaged on my modified Olympus BHB with a...

Cool. As someone who works with UV a lot, a word of advice though. If you are going to use it, please wear UV safety goggles. I'm not sure what wavelengths that light source will emit, but the fact that it is called a short wave microscope makes me thunk UV and UV can be very damaging to the eyes an...

Thanks all. I prefer buying older slides so I keep an eye out for them when I can. So far, they have been nice and robust. I am not a huge fan of immersion oil, and prefer to use glycerine where the objective allows (just because it is so much easier to clean afterwards).

Well this post has moved on since yesterday. Thanks for all the comments, makes for interesting reading. The coating on the outside of the slide is interesting, I hadn't thought of that and assumed it was just on the inside face. I've got a reflection probe for my spectrometer, so next time I am usi...

I would have expected better contrast from such a slide. I have a few of this type, albeit from more modern mounters than Horace Dall. The coating metal is often different too, aluminium being quite common. This is of one by John Dale. Mike From what I read in the Quekett Journal article mentioned ...

Are you using the 0.9 dry? they can be silvered also using the same chemical process as mirrors. Silver nitrate. Super shiny and reflective. Excellent image capture. Things must be fairly well lined up. Coated coverslip. Coated sapphire watch glass I have only found from 1mm x18mm onwards. Yes, it'...

Way out of my depth here, JMC :oops: All the microscopy-related references to titanium dioxide seem to be about nanoparticles … except, of course, those touting slides by Horace Dall Do you have any references to his mounting technique, that you could share please ? Thanks MichaelG. . Just found th...

I recently had a Horace Dall diatom slide arrive on which the diatoms had been mounted with Titanium dioxide. The slide was made in 1950 and this procedure was done to increase their refractive index. I have done some quick test images of the slide (two objectives - Olympus Splan 20x NA0.46, and a Z...

There is something very beautiful about diatom structures so I try and keep an eye out for diatom slides on ebay. I had a couple turn up this week - a 7 form Test Slide from Klaus Kemp and one antique one by John Barnett with a group of 4 Eupodiscus Radiatus diatoms. These are some quick images of t...

Maybe with a vacuum immersion lens. https://www.microbehunter.com/microscopy-forum/viewtopic.php?t=1764 Thanks, I'd not heard of those before. I spoke with a friend of mine in Germany who is a microscope dealer. He thinks it may not even be from a microscope at all, and that it could be from someth...

I need a bit of help identifying a mystery objective please. The person I got it from knew nothing about (it was from ebay from a vendor who dealt in general second hand stuff, but wasn't a microscope dealer). Its mount seems to be the same diameter as RMS but is a finer pitched thread. I could get ...

There is a set of all three of Pluta's books for sale on ebay at the moment; https://www.ebay.co.uk/itm/Advanced-Light-Microscopy-vol-1-2-3-M-Pluta-1988-1989-1993-Unique-rarity/325162950937 I have no affiliation with the seller other than having bought from him before (and it was a good transaction).

MichaelG. wrote: ↑

Tue Apr 26, 2022 6:56 am

Just an historical note which may be of interest:

In the book that I referenced yesterday, there is a letter from Dr. S CZAPSKI, of Jena, to Mr. Van Heurck

pp 357-364

MichaellG.

Thanks MichaelG, yes it's an interesting read that letter.

Hi Louise, For me it's part of my work, so thankfully the research I do covers the costs of the equipment I buy (as long as the work I do for clients gives them what they need, I can carry on doing the interesting science). Yes, it was a big investment, but I hope to use the camera for my microscopy...

Hi JMC - is this perchance your article? https://www.google.com/search?q=nikon+d800+monochrome+uv+spectral+sensitivity&sxsrf=APq-WBuNvzSfxvLhRAEZxS-HeE_AWflbaw%3A1650755585967&ei=AYhkYqPVOtqA8gLi3pCICw&ved=0ahUKEwjj2b_ip6v3AhVagFwKHWIvBLEQ4dUDCA4&oq=nikon+d800+monochrome+uv+spectral+sensitivity&gs_...

Apochronaut - thank you for that.

MichaelG - yes, I bought it from Par.

I've played around with a reflecting objective that has no name on it whatsoever but my impression is that it may be a Cooke. The results were similar to that which you achieved in your comparison, which I have guessed in my case to be a result of a poor condenser set up and poor cover glass design...

Thanks all for the comments. MichaelG - yes I've got a few of the old Beck ones. They have no glass in them - they're just mirrors and are great from UV through IR. I have done some imaging at 313nm with one of them - https://jmcscientificconsulting.com/uv-microscopy-diatom-with-3-different-objectiv...

I was wondering if anyone had any information on a Leitz objective that I have, as so far I've not been able to find anything out about it? The objective is a Leitz 40x Spiegel (reflecting) objective. NA 0.65. RMS mount and 170mm tube length and made for a 0.17mm coverslip. Attached are a couple of ...

I have a few different, and somewhat unusual items, which I'm looking for for my UV microscopy work. I'll put a post per item up if that is ok. First one, a Leitz 50x NA 1.00 Pv Quarzgl 0.18mm glycerine immersion objective. This was originally for a microspectrograph, but I am hoping someone perhaps...