MicrobeHunter.com Microscopy Forum

Excellent detail as usual! Just curious what instrumentation do you use in your evaluations. I have always been curious, especially in color renditions, as to how comparisons are made. I know in photography we have master color charts, e.g. Tiffen Color Separation Guide and Grey Scale. Are there sim...

With the exception of only a few early infinity corrected apochromats that I know of ( 10X .30, 43X .80 and 80X 1.30) the infinity corrected objectives have the catalogue # engraved on the objective barrel. They are rarely 3 numbers and normally 4 numbers. There were rarely serial #'s on the infini...

AO objectives labelled as C (C=coated) are for 160mm TL systems. Have you considered adding a pair of 15x eyepieces? Your 40x then gets you to 60x. Edit: There's a 63x 34mm inifinity in this post https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=16&t=15484 Thanks for the link, but too ...

I'm trying to stay away from oil, and the 34mm parfocal infinity objectives are hard to come by, it seems, except for the standard 4x, 10x, 43x/45x, 97x/100x oil. I came across a 20x AO Spencer C80874 but don't know whether it is Infinity corrected. I've tried to search, but no cigar. I appreciate y...

Thank you, both. Some very interesting insights for me to ponder!

Looking to buy AO Spencer 60x Dry Objective Cat # 122 (infinity corrected) at a reasonable price (only have one arm and leg left!! I'm in USA so prefer sale here to avoid high shipping costs.

It seems that there are two "standard" industrial parfocal lengths used, 45mm and 60mm. AO Spencer used 34mm. Can spacers be used to adapt a shorter parfocal length objective to a turret with longer parfocal length objectives? For example, 11mm spacer with 34mm parfocal objective to obtain 45mm leng...

If you want to display it, get some Lexan or Plexiglas and make a display case for it. You could also find an appropriately sized fish tank and set it upside-down over your 'scope on a wooden platform. Just some suggestions.

Welcome aboard; many knowledgeable people here willing to share their knowledge. Enjoy your journey!!

On the X-Y units on my 'scopes there is a small nylon pad on the bottom so that the actual mechanism does not actually rub on the table when in motion. Perhaps some accumulation of debris under the pad has lifted the mechanism enough for it to slip over the slide. Just a thought.

I'm not 100% sure as I bought mine used thru Ebay, but I think these are the people I bought mine from:
https://spwindustrial.com/swift-optical ... field-10x/
Price is way above what I paid for mine, but they have 5 for sale.

The copy of the manual that I have states that the condenser N.A. is 1.2, but the .65 N.A. sounds more realistic. Also, I read that the eyepieces were 10X though the manual does state them to be 12.5x. That would only change the Magni-Changer lens magnifications in any case. The two small knobs just...

The popularity if Swift microscopes is based on several elements. James Swift and Son was a venerable innovative English microscope maker, which made a number of the most classic British microscopes. As the British industry faltered, the Swift trademark went to another company but ultimately ended ...

I actually bought two AO Sixty microscopes "for parts or repair" thinking I could make one good 'scope from the two. When I received them, they were both in near perfect working condition and fairly nice cosmetically, as well. One had a three objective turret w/ 4x,10x, and 43x objectives, a 161 wid...

In my newcomer intro, I mentioned that I had purchased a Swift SW200 'scope mainly because of the good reviews it received and the review that stated it was "not a toy but a good entry level microscope." Well my assessment is that it is a good 'scope for grade school and maybe jr. high. It is a very...

Just curious about the use of oil on the condenser. I have an AO Sixty Reference Manual, which goes through the details of how to use the 100x oil objective, and it does not include anything about putting oil on the condenser and specifically shows only adding oil to the cover slip. Everything I rea...

I have a couple of AO Sixty microscopes and found a Reference Manual for it that had each page in .jpg format. I printed each page and then scanned it, so I now have a copy in .pdf format, which makes it much easier to use when viewing on the computer. If anyone would like a copy of the .pdf manual,...

Welcome to the forum. I'm relatively new here, as well, and have found many knowledgeable people willing to share their knowledge. The 'scopes you mentioned are good, but a lot depends on the objectives that are included with them. The higher the quality of the objectives, the higher the cost of the...

viktor j nilsson wrote: ↑

Mon Jun 06, 2022 11:40 am

GerryR wrote: ↑

Mon Jun 06, 2022 10:41 am

haileynelson10 wrote: ↑

Mon Jun 06, 2022 9:25 am

Parfocalling is exactly what you have explained. Some people sometimes are still wrong about it.

Please clarify; who explained it properly and what do people get wrong about it? Much appreciated. Thanks.

It's a spam bot.

You might think people would have something better to do.

haileynelson10 wrote: ↑

Mon Jun 06, 2022 9:25 am

Parfocalling is exactly what you have explained. Some people sometimes are still wrong about it.

Please clarify; who explained it properly and what do people get wrong about it? Much appreciated. Thanks.

Nice post! Two books I have that I don't see on your "shelf" are the following:
Principles & Techniques in Histology, Microscopy and Photomicrography; D.R. Singh
Exploring with the Microscope; Werner Nachtigall

Thanks for all the inputs; great suggestions, and I definitely learned a better way of doing this!!

I also shim at objective shoulder using o rings or rubber bands.Tension provided by screwing action and rubber bands is enough to hold objective at the exact shim height. No glue is needed. Excellent solution! All this works really well if you have to bring the objective closer to the specimen; wha...

Not. There are some designs such as the Bausch & Lomb patented ....... The objective with closest focus is treated as the standard and the other objectives are shimmed to match. Interesting that they start with "the objective with closest focus," which makes sense, as that would seem to be the most...

I have seen many posts where it was suggested that replacing an objective will result in it possibly not being parfocalled. However, I have also read some articles on parfocalling, and it seems rather straight forward: 1) After removing the lower objective tube covers on the higher power objectives ...

The first two are expensive because they are "Nikon" and "Olympus." The third is less expensive because it is "used," and the last one is inexpensive because they cant figure out where the battery goes :roll: By the way, I have used one of the inexpensive ones and they work acceptably well, even wit...

The different it makes is whether or not you want to work with chemicals to eliminate the parafine from the specimen, and whether or not you can get good results with a Carrot. So if I were you I would start with a carrot because that's easy, and go from there. Thank you Greg, I'll start with carro...

I had some time on my hands, so decided to make a microtome (I have a small machine shop.) Nothing fancy. Fabricated out of aluminum with a hardened steel top and steel “plunger" with a 3/8-32 thread for a 0.03125 per revolution advance. I had an old Weck straight razor that uses the longer, single-...

500 WVDC is good to know, but you need to know the capacitance in uf (microfarads). Most items, if not specialized for a particular application can be purchased form, Allied Electronics, Newark Electronics, Digi-key, etc. The value in uf is important and can be gotten from a schematic, or from someo...

Motic still sells the M3 version of this, but they modified it with three micro objectives (if I recall, 4x, 10x, 40x) and a 1x macro. Not nearly as "nifty" IMO as the M2 with the "Magnachanger."