MicrobeHunter.com Microscopy Forum

I highly recommend searching for and observing autofluorescence phenomena, just because of the amazing colors you can admire. Regards Maciej Last September I was given a complete Xenon fluorescence system for my Nikon scope by a friend who runs a university lab and had decommissioned the equipment....

Might that be a girdle view of a cenrtric diatom? You're fast approaching that point where you spend untold hours trying to identify these! It very well could be a girdle view. I shot many diatoms on an angle where it was clear that there were two centric halves of a diatom connected to each other ...

Very beautiful. I should search for more autofluorescent subjects.

Focus stack from video.

Thanks everyone for the comments

Slideshow - A selection of marine diatoms collected with a homemade plankton net (75 micron) from Koh Kut in the Gulf of Thailand. All images are focus stacks (Helicon Focus, Method B) from short video clips traveling through focus. Darkfield images using a 3-d printed patch stop: Nikon 10/0.25 Plan...

So much great about the video. Excellent videography, informative labeling and very funny soundtrack.

Luis Carlos wrote: ↑

Wed May 01, 2024 11:25 am

What is observed is the detachment of a gas vacuole. Arcellae use this type of vacuoles to regulate their buoyancy, to escape or to turn his theca

Thank you for that information.

MichaelG. wrote: ↑

Wed May 01, 2024 5:34 am

Nicely done

MichaelG.

Thanks

Sure Squintsalot wrote: ↑

Wed May 01, 2024 5:11 am

Seriously lively! Were you using a well slide?

No, a regular slide with a 24x50mm coverslip -- lots of lateral room to look around.

A closeup look at Suctorian tentacle movement. Suctorians have a spiral structure around their tentacles that acts like an accordian, moving the tentacles in and out.

Playback at 8x speed to better show this movement, which is very slow in real time.

This was a pretty active slide, including 2 Lacrymaria feeding.

Footage sped up (as noted on each clip).

Freshwater sample from Lumpini Park, Bangkok, Thailand.

Hobbyst46 wrote: ↑

Thu Apr 25, 2024 8:39 pm

Onion skins are nice to look at and are worth the effort IMO. One simple way to prepare them is as follows.
1. Clea . . .

Very nice and detailed tutorial, thanks.

Huge bloom of Euglena sanguinea, imaged at 10x darkfield, 20x, and 40x oil.

Freshwater sample from Lumpini park, Bangkok, Thailand.

The red color is due to the presence of the carotenoid astaxanthin, an antioxidant. Astaxanthin is responsible for the pink-red color of salmon.

Sure. In the first shot you see an egg attached to a rotifer. The baby rotifer has begun breaking out of it's egg. Each successive shot shoes the baby rotifer breaking further out of it's egg., until at the end it has broken free and immediately begins feeding.

Very cool!

Freshwater sample from Lumpini Park, Bangkok, Thailand.

This process took about 20 minutes in real time.

Definitely a very active biome!

Francisco wrote: ↑

Sat Apr 20, 2024 3:45 pm

Pleuronema

Very nice detail

apochronaut wrote: ↑

Thu Apr 18, 2024 12:45 pm

You could set up a Euglena smoothee concession. Could be the next Spirulina ! Might tickle on the way down, though.

LOLOL

Freshwater sample from Lumpini Park, Bangkok, Thailand.

Pond water sample from Lumpini Park, Bangkok, Thailand.

Really nice 3d effect and detail.

Ok thanks. Brought down by the old double flagella!

We see an ovoid heterotrophic euglenoid with a strongly ribbed pellicle, but I can't make out an ingestion apparatus or any flagella. I don't see how we can rule out a strongly ribbed biflagellate, like certain species of Ploetia and Entosiphon . Is there a particular species of Petalomonas you had...

At various frames in the videos, when I look frame by frame, a flagellum comes into focus. I'll grab a screenshot when home. I was hoping to ID to at least the genus level for the BioBlitz project but I had thought I probably don't have enough detail. I had thought the ribs, along with the barrel sh...

Is this the flagellate Petalomonas?

Absolutely awesome video. I find it difficult to record Condylostoma, you've done it very well.

Sure Squintsalot wrote: ↑

Wed Apr 03, 2024 6:02 pm

Cool video.

Does your rig not support darkfield?

I saw another one, so I shot it with a Rheinberg filter widefield.