MicrobeHunter.com Microscopy Forum

A standard rock crusher or a home made one will not crush the fossil fine enough to use with a transmitted light microscope. Crushed rock or fossils could be viewed quite nicely with a reflected light stereo microscope. To view a crushed specimen with transmitted light, the crushed rock would have t...

mrsonchus: My experience is almost the exact opposite of yours. I started out with a 3MP 5MP and then a 10MP USB Toupcam camera. All of them are less than stellar when compared to my two Canon cameras. If I intend to make large (A1) size prints from my micro-photos I use a Canon 5D MkII (21MP) on my...

I have had a mixed experience with hi-power LEDs for microscope illumination. I am presently on my fourth unit. The previous three have all been returned for credit due to the same problem. When the LED power is increased to maximum or near maximum a Venetian blind effect is visible in the image. I ...

Astyanax: The Amscope V-T720 is a full featured microscope and has a field iris. You will be able to set Koehler illumination. Your Tucsen USB camera is a good unit and coupled with the Amscope objectives will give excellent results. The Tucsen camera has a fixed focal length and is ideally suited f...

I have a few fossils, not very rare, I am going to grind and polish into thin sections.
Are you planning on making thin sections from some of your collection?

The first one for the grinder is a trilobite.

Roswell is not a hoax. Created in Maya with thin section rock photos as maps.
My secondary use of microscope photos is as bump and texture maps.

After the above responses, I tried making a mirrored sphere with flashing lights.
But the closest I could come with short notice is Potassium Ferricyanide, Topas Labs star filter, a few 60's friends and a quick render in Vue Esprit.

DO NOT WATCH THIS ANIMATION IF YOU ARE SUSCEPTIBLE TO CONVULSIONS.
and you have a fast download.

This GIF animation was assembled from 12 photos of Aeolian sandstone as the stage was rotated through 90 °.
A lambda plate was in place and a 2x objective.

Lymia:

You adjust the colour balance in your Nikon camera.
Set the white balance to Tungsten. That will clear up your photos and movies.

Lymia: This is essentially what JimT is describing . 1 - Place your Nikon camera on a tripod, 2 - Remove the lens. 3 - Attach one or two manual extension tubes. Manual extension tubes Manual extension tubes.jpg The tubes are inexpensive, buy two. 4 - Place your 10x eyepiece in your microscope. Turn ...

Lymia: The photos are meant to show: a) The difference in magnification between a 10x eyepiece and a 2.5x PL eyepiece b) The difference in quality between an excellent eyepiece (2.5x PL) and the eyepiece (10x WF) that comes with a standard microscope. Both photos were taken with an old Canon Rebel X...

Lymia: I have attached two photos comparing a 2.5x Nikon Projection lens with a 10WF eyepiece.The photos are of the same location on the slide. The original photos are RAW full size images for the camera resampled to 640x480 for the forum. You will immediately notice the larger magnification with th...

Your darkfield condenser is designed to be used only with the 100x obj. If your specimen is properly prepared, the 20w halogen lamp should provide enough illumination. Use your brightfield condenser with a lower magnification obj. and place a darkfield stop in the filter holder. These articles from ...

Lymia: Your Nikon camera is vastly superior to any USB camera you can purchase under $2000.00. The adapter you are currently using is more than adequate for your needs. The height of the condenser is adjusted to give even illumination across the aperture of the objective, not to adjust the amount of...

Lymia: There is no part missing from your microscope. Your microscope does not have a photo tube option and there is not microscope in your model line that has a photo tube. I stated the setup may be unwieldy because the length of the camera adapter tube is long and with the camera on the end would ...

Lymia: H.264 is a video format. Your camera supports two formats: H.264 (Best) and MPEG-4 (Normal) in three resolutions, 1920, 720, 424 at several frame rate per second (fps). Look in the camera manual for the video or videography section. It will explain how to set your camera for various video qua...

Lymia: Your Nikon d3300 has a 24MP APS-C sensor and is capable of taking full HD movies. Please note, your still photos (JPEG, RAW) have a resolution of 6000 x 4000 pixels. HD movies have a resolution of 1920 x 1080 pixels. Your still photo images have considerably more resolution than your movies. ...

Elucidation: The editor is HM my wife. I am making some thin section mineral slides and documenting the process. Thin section mineral slides consist of very thin 30-50 micron slices of rock mounted on a glass slide. I described an earlier process making a thin section by hand. The latest adventure n...

Lymia: The microscope and camera adapter you have should take excellent photos that are a bit soft around the outer edges. The camera adapter replaces the lens of your camera. The camera should be set to Aperture Priority and let the camera set the speed. The camera sees the adapter as a 0 f stop le...

I am in the process of writing and photographing a "How I make thin sections" of domestic rocks.
Not exactly in your topic category, but they are sections. Some thin, some not and some will have to be recovered from the dust bin.

The text must first pass the local editor.

Two thin sections. One a dancer with a boring name, the other a wallflower with a pretty name.

Silicalite sounds so ordinary and Coquina so coquettish. Yet Silicate photographs well and even a Lambda plate does not help Coquina.

Silcate and Coquina presentation.

Use Microsoft Word 97 , the .DOC format for compatibility and not the newer .DOCX format. It is probably better to insert place holder images in the document and attach the full size photos. The Editor asks that your photos be in JPEG format and full size. Not overly sharpened (my advice). The above...

A few years ago I purchased a Caswell Plug N' Plate Gold Kit. After use there was a small portion remaining. I forgot all about the kit until this Spring cleanup session. Upon shaking the bottle, I heard something rattling inside. Using tweezers I extracted what looked like three small pieces of gla...

The Spring season with its pretty colours is very short here and quickly morphs into Summer.
These are few photos I took of the early Spring colours and preserved them forever in stone.
Spring colours

BillBillt:
The linked URL.
Pay careful attention to the syntax . You need the full URL including the "https://"
Hook's Micrographia

You can download the ebook at Hook's Micrographia ebook

You may be interested in this Background Subtraction Toolkit developed expressly for microphotographs.
Download
and
Usage

allow me to see in Darkfeild,

WayOfWater:

The camera does not allow you to see Darkfield illumination, it allows you to see in low light situations.
Darkfield is achieved using special Darkfield stops below or in the condenser.

Just being nit picky.

Welcome to the forum.

Browsing the thin section of Sandstone Aeolian I noticed a couple of items that seemed out of place.
My first thoughts were artifacts, but they proved to be part of the specimen.
Sandstone (Aeolian)

The gallery presentation shows what I have managed to this point. They are not the greatest thin sections, but I am improving. At least the failure rate has dropped if that is any indication.
The thin sections

Ledikite..
Not all that common. There is some disagreement about the name.
A silica mineral of the mica family. Similar to Biotite.