MicrobeHunter.com Microscopy Forum

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Thanks Hobbyst46, looks like I am going to start measuring slide thickness as well now.

Found this in Light Microscopy by Murray and Robinow page 15
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Thanks

Found this in Fundamentals of light microscopy
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It suddenly occurred to me that though I do take into account cover slip thickness I have not done the same with slide thickness.

Both my slide boxes have 1.0/1.2mm written on them. Though I have not measured the slides. Is there an ideal thickness?

Thanks Wes An eyepiece is designed to send a virtual image that needs a lens (afocal or an eye) to convert it into a real one. By raising the eyepiece it produces instead a real image that can then be projected directly onto the camera sensor. This is to try to improve sharpness and detail (closer t...

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Thanks MichaelG. and Hobbyst46

Is the 12.5X KPL raised above parfocality height ?
Is it a 12.5x -W high eyepoint KPL ?

Raised 10mm by the collar shown. High eyepoint (as indicated by the eyeglass pictogram) but believe field of view is 18mm
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Just to add to the comments above - You may find that though the generic phase kit works well enough you will be limited by the objectives that come with it. A used 160 finite quality microscope will allow you to upgrade just about everything in time (and all designed to work well together) To use Z...

Thanks janvangastel, was experimenting with direct projection using a 12.5x Kpl

Many thanks sinabro

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Zeiss Planapo 63x/1.4, DIC, 70µm diameter, stacked in Photoshop, Marine sample, Alboran Sea.
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Very nice! Great resolution and clarity

Many thanks janvangastel and ImperatorRex (had missed your posts)

Great videos with some very nice detail - particularly liked the hatching!

Hi, you might have a look at Rolf's new camera adaptation method: https://microscopyofnature.com/microphotography-hybrid-eyepieces He uses combinations of standard eyepiece components to form a projective. I have tested his method and it works very well. Bob The modified eyepiece idea as shown by R...

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Excellent action video. Do hope Frank recovers soon!

Thanks Plasmid

Many thanks Luis Carlos, D0c and Hobbyst46

Thanks MicroBob and great link. Always nice to see beyond what a light microscope can do!

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Zeiss Planapo 63x/1.4, DIC, 65µm length, stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Unfortunately some stem detail was lost in the stacking
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Many thanks LouiseScot and KurtM

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Came across a small strand of Melosira nummuloides yesterday, always a treat when I come across them

Zeiss Planapo 63x/1.4, DIC, 17µm width, stacked in Photoshop, Marine sample, Alboran Sea.
Second image included a stitch as well, in order to capture the whole strand.

very nice images, well done

Here's a diatom strew with some crops using the Nikon 40/0.85 and 3W violet-blue LED. I've been testing out the violet-blue LED though I find it strange that it looks violet-blue to the naked eye but turns into a deep blue when viewed through an ocular. For my peace of mind - What precautions are y...

Hobbyst46 wrote: ↑

Sat Apr 03, 2021 3:02 pm

Fantastic diversity.
From which depth (in the mud, and below the water) was the sand collected ?

I used a jam jar to scoop the wet sand from the surface down to 2 - 3 cm at low water.

You might find this link useful. You can set it for your area

https://www.tidetime.org/europe/spain/estepona.htm

Nice variety! - including a few I have not come across myself.

Thinking of following your example ...

When you mentioned that you 'swirl the water and then decant', is that by hand or with a centrifuge?

Yes, only one is used.

I use one (calibrated with a stage micrometer) to measure length and width with but as I find it distracting I have it placed in an additional (i.e. a third) eyepiece.

Welcome j almeida,

do post some photos of your microscope in the 'My microscope' section when you have a moment. I am sure we would all like to see them.

Looking forward to your diatom photos!

Great catch and video!

It can be a challenge to find and clean dust inside a microscope. Fortunately eyepieces are one of the easiest to isolate - if the dust spots turn as you spin the eyepieces in place you have found the culprit. Setting up Köhler precisely helps to 'hide' some of the inevitable dust. Also make sure yo...

Thanks. Nice read!

Great catch!

Curiously it reminded me that I need to finally get around to cleaning my sensor ;)