Search found 7671 matches

by 75RR
Mon Apr 19, 2021 9:28 pm
Forum: Microscopes and optics
Topic: Is Slide thickness critical?
Replies: 5
Views: 78

Re: Is Slide thickness critical?

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Thanks Hobbyst46, looks like I am going to start measuring slide thickness as well now.

Found this in Light Microscopy by Murray and Robinow page 15
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by 75RR
Mon Apr 19, 2021 5:47 pm
Forum: Microscopes and optics
Topic: Is Slide thickness critical?
Replies: 5
Views: 78

Re: Is Slide thickness critical?

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Thanks

Found this in Fundamentals of light microscopy
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by 75RR
Mon Apr 19, 2021 4:53 pm
Forum: Microscopes and optics
Topic: Is Slide thickness critical?
Replies: 5
Views: 78

Is Slide thickness critical?

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It suddenly occurred to me that though I do take into account cover slip thickness I have not done the same with slide thickness.

Both my slide boxes have 1.0/1.2mm written on them. Though I have not measured the slides. Is there an ideal thickness?
by 75RR
Mon Apr 19, 2021 4:03 pm
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 174

Re: Actinocyclus octonarius

Thanks Wes An eyepiece is designed to send a virtual image that needs a lens (afocal or an eye) to convert it into a real one. By raising the eyepiece it produces instead a real image that can then be projected directly onto the camera sensor. This is to try to improve sharpness and detail (closer t...
by 75RR
Mon Apr 19, 2021 1:10 pm
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 174

Re: Actinocyclus octonarius

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Thanks MichaelG. and Hobbyst46
Is the 12.5X KPL raised above parfocality height ?
Is it a 12.5x -W high eyepoint KPL ?
Raised 10mm by the collar shown. High eyepoint (as indicated by the eyeglass pictogram) but believe field of view is 18mm
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by 75RR
Mon Apr 19, 2021 7:39 am
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 5
Views: 171

Re: should i buy Bresser phase contrast series 57?

Just to add to the comments above - You may find that though the generic phase kit works well enough you will be limited by the objectives that come with it. A used 160 finite quality microscope will allow you to upgrade just about everything in time (and all designed to work well together) To use Z...
by 75RR
Mon Apr 19, 2021 7:16 am
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 174

Re: Actinocyclus octonarius

Thanks janvangastel, was experimenting with direct projection using a 12.5x Kpl
by 75RR
Mon Apr 19, 2021 5:49 am
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 174

Re: Actinocyclus octonarius

Many thanks sinabro
by 75RR
Sun Apr 18, 2021 11:52 pm
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 174

Actinocyclus octonarius

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Zeiss Planapo 63x/1.4, DIC, 70µm diameter, stacked in Photoshop, Marine sample, Alboran Sea.
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by 75RR
Wed Apr 14, 2021 6:19 am
Forum: Pictures and Videos
Topic: Rotifer Euchlanis
Replies: 5
Views: 191

Re: Rotifer Euchlanis

Very nice! Great resolution and clarity
by 75RR
Tue Apr 13, 2021 3:28 pm
Forum: Pictures and Videos
Topic: Licmophora abbreviata
Replies: 9
Views: 267

Re: Licmophora abbreviata

Many thanks janvangastel and ImperatorRex (had missed your posts)
by 75RR
Sun Apr 11, 2021 12:09 am
Forum: Pictures and Videos
Topic: Rotifer: Adineta vaga minor
Replies: 3
Views: 163

Re: Rotifer: Adineta vaga minor

Great videos with some very nice detail - particularly liked the hatching!
by 75RR
Sat Apr 10, 2021 12:50 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 594

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

Hi, you might have a look at Rolf's new camera adaptation method: https://microscopyofnature.com/microphotography-hybrid-eyepieces He uses combinations of standard eyepiece components to form a projective. I have tested his method and it works very well. Bob The modified eyepiece idea as shown by R...
by 75RR
Fri Apr 09, 2021 6:41 pm
Forum: Pictures and Videos
Topic: Hydra viridissima gets decimated.
Replies: 8
Views: 317

Re: Hydra viridissima gets decimated.

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Excellent action video. Do hope Frank recovers soon!
by 75RR
Fri Apr 09, 2021 6:40 pm
Forum: Pictures and Videos
Topic: Licmophora abbreviata
Replies: 9
Views: 267

Re: Licmophora abbreviata

Thanks Plasmid
by 75RR
Fri Apr 09, 2021 12:16 pm
Forum: Pictures and Videos
Topic: Licmophora abbreviata
Replies: 9
Views: 267

Re: Licmophora abbreviata

Many thanks Luis Carlos, D0c and Hobbyst46
by 75RR
Fri Apr 09, 2021 12:14 pm
Forum: Pictures and Videos
Topic: Melosira nummuloides
Replies: 5
Views: 152

Re: Melosira nummuloides

Thanks MicroBob and great link. Always nice to see beyond what a light microscope can do!
by 75RR
Fri Apr 09, 2021 7:40 am
Forum: Pictures and Videos
Topic: Licmophora abbreviata
Replies: 9
Views: 267

Licmophora abbreviata

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Zeiss Planapo 63x/1.4, DIC, 65µm length, stacked and stitched in Photoshop, Marine sample, Alboran Sea.

Unfortunately some stem detail was lost in the stacking
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by 75RR
Fri Apr 09, 2021 6:20 am
Forum: Pictures and Videos
Topic: Melosira nummuloides
Replies: 5
Views: 152

Re: Melosira nummuloides

Many thanks LouiseScot and KurtM
by 75RR
Thu Apr 08, 2021 11:57 pm
Forum: Pictures and Videos
Topic: Melosira nummuloides
Replies: 5
Views: 152

Melosira nummuloides

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Came across a small strand of Melosira nummuloides yesterday, always a treat when I come across them

Zeiss Planapo 63x/1.4, DIC, 17µm width, stacked in Photoshop, Marine sample, Alboran Sea.
Second image included a stitch as well, in order to capture the whole strand.
by 75RR
Tue Apr 06, 2021 7:46 am
Forum: Pictures and Videos
Topic: Collotheca sp. - Rotifer
Replies: 12
Views: 1601

Re: Collotheca sp. - Rotifer

very nice images, well done
by 75RR
Sun Apr 04, 2021 7:58 pm
Forum: Illumination Techniques
Topic: First go at Dodt
Replies: 2
Views: 193

Re: First go at Dodt

Here's a diatom strew with some crops using the Nikon 40/0.85 and 3W violet-blue LED. I've been testing out the violet-blue LED though I find it strange that it looks violet-blue to the naked eye but turns into a deep blue when viewed through an ocular. For my peace of mind - What precautions are y...
by 75RR
Sat Apr 03, 2021 4:16 pm
Forum: Specimens, samples and slides
Topic: Alboran Sea Diatoms
Replies: 11
Views: 382

Re: Alboran Sea Diatoms

Hobbyst46 wrote:
Sat Apr 03, 2021 3:02 pm
Fantastic diversity.
From which depth (in the mud, and below the water) was the sand collected ?
I used a jam jar to scoop the wet sand from the surface down to 2 - 3 cm at low water.

You might find this link useful. You can set it for your area

https://www.tidetime.org/europe/spain/estepona.htm
by 75RR
Sat Apr 03, 2021 2:25 pm
Forum: Specimens, samples and slides
Topic: Alboran Sea Diatoms
Replies: 11
Views: 382

Re: Alboran Sea Diatoms

Nice variety! - including a few I have not come across myself.

Thinking of following your example ...

When you mentioned that you 'swirl the water and then decant', is that by hand or with a centrifuge?
by 75RR
Wed Mar 31, 2021 12:51 pm
Forum: Microscopy accessories
Topic: How many reticles needed?
Replies: 2
Views: 237

Re: How many reticles needed?

Yes, only one is used.

I use one (calibrated with a stage micrometer) to measure length and width with but as I find it distracting I have it placed in an additional (i.e. a third) eyepiece.
by 75RR
Mon Mar 29, 2021 5:39 pm
Forum: Introduce yourself
Topic: Hello from Salvador, Bahia
Replies: 4
Views: 218

Re: Hello from Salvador, Bahia

Welcome j almeida,

do post some photos of your microscope in the 'My microscope' section when you have a moment. I am sure we would all like to see them.

Looking forward to your diatom photos!
by 75RR
Mon Mar 29, 2021 4:09 pm
Forum: Pictures and Videos
Topic: Ciliate during cell division
Replies: 5
Views: 221

Re: Ciliate during cell division

Great catch and video!
by 75RR
Sun Mar 28, 2021 9:24 am
Forum: Pictures and Videos
Topic: Tintinnidium in it's lorica?
Replies: 5
Views: 214

Re: Tintinnidium in it's lorica?

It can be a challenge to find and clean dust inside a microscope. Fortunately eyepieces are one of the easiest to isolate - if the dust spots turn as you spin the eyepieces in place you have found the culprit. Setting up Köhler precisely helps to 'hide' some of the inevitable dust. Also make sure yo...
by 75RR
Sun Mar 28, 2021 8:10 am
Forum: Resources (online, books etc.)
Topic: Fungi / Kew Seed Bank
Replies: 2
Views: 275

Re: Fungi / Kew Seed Bank

Thanks. Nice read!
by 75RR
Sun Mar 28, 2021 7:56 am
Forum: Pictures and Videos
Topic: Tintinnidium in it's lorica?
Replies: 5
Views: 214

Re: Tintinnidium in it's lorica?

Great catch!

Curiously it reminded me that I need to finally get around to cleaning my sensor ;)