MicrobeHunter.com Microscopy Forum

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Thanks ScienceMatters, I am happy with it. It is just that for me, part of the fun is seeing how far I can get.

I get strong chromatic aberration especially around the edges when I tried direct projection without lifting the eyepiece. Hi Wes, in this case you will have changed the objective's focus, right? This is not a good method as the objectie is not used as intended. Bob What I did was to make sure the ...

I compared Rolf's hybrid eyepieces ( eye lens KPL 10x, barrel KPL 8x, field lens either KPL 8x or KPL 10x) to a lifted standard KPL 8x: The hybrids give a much better image. So Rolf really had an ingenious idea here, especially since the hybrids consist of fairly common components. Neither of us ha...

. Many thanks chrisimbee and MicroBob For your M43 camera the lenses of a KPL 10x non-high eyepoint mounted on the barrel of the KPL 8x non-high-eyepoint should give good colour correction and good use of the image circle. That was an interesting article: https://microscopyofnature.com/microphotogra...

Many thanks ImperatorRex

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That is very good for Brightfield, well done.

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Thanks sinabro

Thanks for the tip!

Thanks apochronaut, was just trying to keep things like postage and customs simple.

Have dropped the UK as an ebay shopping area - have heard lots of horror stories in terms of postage and tax!

Will contact some EU dealers and see what they have.

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Wonder if what you have there is Hyperamoeba flagellata.

See link: https://www.researchgate.net/publicatio ... _apparatus

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Anyone know of a European supplier for objective shims?

Link above may charge European buyers in Euros but the company is in Philadelphia USA

That is a great resource + beautiful images - thanks for the hard work!

Welcome BobHelmond, when you say new trinocular, new to you or new new?

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Thanks Hobbyst46, not something I have managed to catch on video before as I usually start by pinning them down with the cover slip.

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Quite a lot of water between the diatom and the cover slip at times with consequently a drop in resolution
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Sometimes quite a lot of detail is needed for an ID

Are you familiar with Ferry's website Microworld?

https://www.arcella.nl/lobose-testate-amoebae/

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Thanks Hobbyst46, looks like I am going to start measuring slide thickness as well now.

Found this in Light Microscopy by Murray and Robinow page 15
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Thanks

Found this in Fundamentals of light microscopy
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It suddenly occurred to me that though I do take into account cover slip thickness I have not done the same with slide thickness.

Both my slide boxes have 1.0/1.2mm written on them. Though I have not measured the slides. Is there an ideal thickness?

Thanks Wes An eyepiece is designed to send a virtual image that needs a lens (afocal or an eye) to convert it into a real one. By raising the eyepiece it produces instead a real image that can then be projected directly onto the camera sensor. This is to try to improve sharpness and detail (closer t...

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Thanks MichaelG. and Hobbyst46

Is the 12.5X KPL raised above parfocality height ?
Is it a 12.5x -W high eyepoint KPL ?

Raised 10mm by the collar shown. High eyepoint (as indicated by the eyeglass pictogram) but believe field of view is 18mm
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Just to add to the comments above - You may find that though the generic phase kit works well enough you will be limited by the objectives that come with it. A used 160 finite quality microscope will allow you to upgrade just about everything in time (and all designed to work well together) To use Z...

Thanks janvangastel, was experimenting with direct projection using a 12.5x Kpl

Many thanks sinabro

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Zeiss Planapo 63x/1.4, DIC, 70µm diameter, stacked in Photoshop, Marine sample, Alboran Sea.
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Very nice! Great resolution and clarity

Many thanks janvangastel and ImperatorRex (had missed your posts)

Great videos with some very nice detail - particularly liked the hatching!

Hi, you might have a look at Rolf's new camera adaptation method: https://microscopyofnature.com/microphotography-hybrid-eyepieces He uses combinations of standard eyepiece components to form a projective. I have tested his method and it works very well. Bob The modified eyepiece idea as shown by R...

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Excellent action video. Do hope Frank recovers soon!