Search found 233 matches

by Tom Jones
Wed Jul 20, 2022 6:16 pm
Forum: Microscopes and optics
Topic: Why is DIC so Expensive?
Replies: 10
Views: 375

Re: Why is DIC so Expensive?

Probably both. It's a research technique, and that implies a very small market. Research is generally funded through grants and that tends to support higher prices. DIC was never intended to be an amateur technique, but I suspect now more amateurs utilize it than researchers, given the advent of new...
by Tom Jones
Tue Jul 19, 2022 10:36 pm
Forum: Camera systems and imaging
Topic: Camera won't take pictures
Replies: 3
Views: 169

Re: Camera won't take pictures

Break out you camera manual. Somewhere in there is a function to allow shutter release without a lens. Enable it. It's disabled by default since normally you take pictures with a lens. Obviously your adapter doesn't tell the camera it is a lens. Also see: https://www.dpreview.com/forums/thread/4437376
by Tom Jones
Fri Jul 15, 2022 2:35 pm
Forum: Specimens, samples and slides
Topic: Interesting bacteria structures or problem with over heat fixing?
Replies: 3
Views: 225

Re: Interesting bacteria structures or problem with over heat fixing?

Years ago, my first microbiology professor said of heat fixing, to pass it through the flame 2-3 times, smear up, then lay the slide on the back of your (then ungloved) hand. If you heard the sound of sizzling flesh, it was too hot!
by Tom Jones
Sun Jul 10, 2022 4:09 pm
Forum: Specimens, samples and slides
Topic: Dry mounted slides (no coverslip) are hard to see
Replies: 4
Views: 301

Re: Dry mounted slides (no coverslip) are hard to see

In clinical labs dry mounted blood smears get a thin coating of immersion oil over them prior to examination with low power objectives to reduce diffraction. Horrible images otherwise. Body fluid smears and grams stains, too. It acts just like a coverslip. Another drop is added when going up to 100x...
by Tom Jones
Sun Jul 10, 2022 3:59 pm
Forum: Specimens, samples and slides
Topic: Can these be saved?
Replies: 8
Views: 715

Re: Can these be saved?

Nothing. The tissue or blood smear remains fixed to the glass as long as you don't accidentally scrape part of it off! I can't vouch for specimens not originally fixed to the glass slide.
by Tom Jones
Sun Jul 10, 2022 3:56 pm
Forum: Identification help
Topic: Help identifying round worm or nematode?
Replies: 2
Views: 182

Re: Help identifying round worm or nematode?

Probably Dero digitata (Ward and Whipple, Fresh Water Biology, 2nd. Ed., pgs 530-531, and https://worms.myspecies.info/taxonomy/term/936)

Edit: Nice images, too!
by Tom Jones
Sat Jul 09, 2022 2:30 am
Forum: Specimens, samples and slides
Topic: Can these be saved?
Replies: 8
Views: 715

Re: Can these be saved?

Yes. Before I retired I re-coverslipped a couple hundred teaching slides I had made as a student and mounted with Permount. Soak in Xylene if you can. It's a lot faster than the citrus-based solvents. They work, they're just slower. Xylene should loosen the coverslip in a couple of days. Flood the s...
by Tom Jones
Tue Jun 28, 2022 3:13 pm
Forum: Camera systems and imaging
Topic: Mirrorless camera and adapter combination?
Replies: 26
Views: 1153

Re: Mirrorless camera and adapter combination?

Be aware that video usually does not use the full sensor. :cry: Look in your camera specs to see the pixel dimensions and you can calculate the physical size of the part of the sensor being used for the resolution you have chosen. Each of the various video formats will use a different set of pixel d...
by Tom Jones
Sun Jun 26, 2022 2:13 am
Forum: Camera systems and imaging
Topic: Nikon Live view vs actual picture
Replies: 8
Views: 418

Re: Nikon Live view vs actual picture

Have you checked your camera settings to be sure your photos are not being saved as a really low resolution jpg?
by Tom Jones
Thu Jun 16, 2022 11:07 pm
Forum: Pictures and Videos
Topic: Count colonies on mobile phone. Get it done in a few seconds
Replies: 2
Views: 318

Re: Count colonies on mobile phone. Get it done in a few seconds

Just don't use it in clinical medicine in the US as it's legally a medical device and needs FDA 510(K) clearance before it can be used.
by Tom Jones
Mon May 30, 2022 2:56 pm
Forum: Miscellaneous
Topic: These 'New Members'
Replies: 20
Views: 1153

Re: These 'New Members'

Thanks Wes!
by Tom Jones
Sat May 28, 2022 11:31 pm
Forum: Miscellaneous
Topic: These 'New Members'
Replies: 20
Views: 1153

Re: These 'New Members'

It's not that these posts aren't annoying. They certainly are. But requiring an answer to a rather esoteric question doesn't seem like a productive way to screen potential members for bots. They're usually pretty obvious, and I don't find them annoying enough to do anything keep new folks away. This...
by Tom Jones
Sat May 28, 2022 4:02 pm
Forum: Miscellaneous
Topic: These 'New Members'
Replies: 20
Views: 1153

Re: These 'New Members'

Wes wrote: How about "calculate the resolution of a microscope with a 63/1,4 objective coupled to a 0.9 condenser at 550 nm"? I'd venture a guess that most amateurs wouldn't care what the resolution is numerically. Most professional microscope users wouldn't either. I know I've never bothered to cal...
by Tom Jones
Thu May 19, 2022 2:58 pm
Forum: Digital processing
Topic: IC Measure alternative ?
Replies: 4
Views: 995

Re: IC Measure alternative ?

Have you tried running your old software in the Win 10 Compatibility Mode? It's designed to allow older software to run on Win 10.
by Tom Jones
Tue May 03, 2022 3:58 pm
Forum: Beginner's corner
Topic: Do I need to add immersion oil to the condenser too?
Replies: 5
Views: 460

Re: Do I need to add immersion oil to the condenser too?

It's generally not important. For a little perspective, in clinical labs we used a LOT of oil. Every single WBC differential slide, and every single gram stain, was examined under oil immersion at the objective only. Unless the slide had be coverslipped and stored for later pathology review, we didn...
by Tom Jones
Fri Apr 22, 2022 11:52 pm
Forum: For forum members who want to buy and sell equipment
Topic: Sold: Olympus BX60 Polarization Microscope. Portland, Oregon
Replies: 4
Views: 749

Re: Olympus BX60 Polarization Microscope. Portland, Oregon

That's an absolute steal. If I was anywhere near you I'd grab it, and I already have one!
by Tom Jones
Wed Apr 20, 2022 3:30 pm
Forum: My microscope
Topic: I need some sample Salmonella bacteria in microscopic research topics
Replies: 1
Views: 290

Re: I need some sample Salmonella bacteria in microscopic research topics

Have a chat with your university's head of clinical or epidemiological microbiology. They have all the resources you will need.
If you want to do research at a university medical school, you need to go through the proper channels.
by Tom Jones
Wed Apr 06, 2022 4:35 pm
Forum: Beginner's corner
Topic: Protein and Collagen, what scope can see it?
Replies: 12
Views: 1130

Re: Protein and Collagen, what scope can see it?

No offense, but I think you need to go back and reassess what you want to do, and why. You say you want to seen protein and collagen, but some pretty simple things like phase contrast and polarization are a bit over your head. To be brutally honest, until you can be a lot more specific about your qu...
by Tom Jones
Mon Apr 04, 2022 2:46 am
Forum: Pictures and Videos
Topic: Rotifer: Testudinella patina
Replies: 9
Views: 821

Re: Rotifer: Testudinella patina

Nicely done! I appreciate the labels, too. They're quite a bit of work, so most people don't use them.

Tom
by Tom Jones
Thu Mar 17, 2022 12:04 am
Forum: Digital processing
Topic: Vibrant DIC videos
Replies: 9
Views: 2127

Re: Vibrant DIC videos

My Olympus BHS system produces nice dark backgrounds as well as the full range of colors depending on how far the analyzer prism is translated. On the other hand, my Olympus BX system with the DICT slider will not produce a good darkfield effect with or without the 530nm waveplate. Only the full ran...
by Tom Jones
Wed Mar 16, 2022 11:23 pm
Forum: Digital processing
Topic: Vibrant DIC videos
Replies: 9
Views: 2127

Re: Vibrant DIC videos

microscopeboi, For a little bit of introductory homework, see: https://en.wikipedia.org/wiki/Differential_interference_contrast_microscopy https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/dic/dicintro/ https://en.wikipedia.org/wiki/Waveplate https://www.youtube.com/watch?...
by Tom Jones
Tue Mar 08, 2022 10:46 pm
Forum: Camera systems and imaging
Topic: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
Replies: 23
Views: 2228

Re: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS

Anything you output to a 720p system will be limited to 720. The original resolution doesn't matter. Even 8K to 720 still gives 720, and that's pretty low-res when blown up to projection scale. 720 looks pretty good on a laptop screen. If you double the size vertically and horizontally (essentially ...
by Tom Jones
Mon Mar 07, 2022 4:35 pm
Forum: Miscellaneous
Topic: transport of a heavy microscope - how to handle it?
Replies: 14
Views: 2100

Re: transport of a heavy microscope - how to handle it?

ewieckow, As you requested, here are the two I use. The first is a custom one I built out of 1/4" ABS plastic glued together, and 1" urethane foam (I think). The foam is pretty stiff and is glued in place with 3M 77 adhesive. Any slots or holes needed, like for the lamp house, were routed out. Notic...
by Tom Jones
Wed Mar 02, 2022 2:09 am
Forum: Camera systems and imaging
Topic: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
Replies: 23
Views: 2228

Re: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS

What woyjwjl said: From the price/performance point, the M43 camera (Panasonic GH4) + NFK3.3 + U-PMTVC adapter (catalog number U-V210, 037151) provides perfect access to 4K images and videos. That combination, along with DIC on my BHS, is what I used to shoot the 2018 Nikon Small World in Motion Hon...
by Tom Jones
Tue Mar 01, 2022 11:10 pm
Forum: Camera systems and imaging
Topic: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS
Replies: 23
Views: 2228

Re: How to get parfocality of an image from a camera with a microscope eyepiece? / Image quality in OBS

Your D3200 is limited to Full HD per Nikon's website, 4K is not possible. You'll need a camera that will output 4K video to clean HDMI. Your computer screen will need to be 4K as well. That's not happening with an old laptop. Your cam-link clone is probably not 4K either. 4K takes a high-throughput ...
by Tom Jones
Mon Feb 07, 2022 4:46 am
Forum: Miscellaneous
Topic: transport of a heavy microscope - how to handle it?
Replies: 14
Views: 2100

Re: transport of a heavy microscope - how to handle it?

Don't try to carry it all the time. It's too big and too awkward. With that much weight in front of you, it's hard on your back, and stairs will be very dangerous. You will get hurt. I have seven BHTU's, two of which are trinocs, that I transport for outreach events. Six are fitted upright, in custo...
by Tom Jones
Sun Feb 06, 2022 3:47 pm
Forum: Microscopy accessories
Topic: Best counting chamber, micropipette
Replies: 6
Views: 956

Re: Best counting chamber, micropipette

Pasteur pipettes are a little difficult to use with such small volumes. Capillary tubes are better for loading the chambers. Cheaper, too. The thicker cover slips are heavier and less prone to float on top of the specimen if it's viscous. Otherwise, either one works fine. Unless you get one with the...