MicrobeHunter.com Microscopy Forum

Probably both. It's a research technique, and that implies a very small market. Research is generally funded through grants and that tends to support higher prices. DIC was never intended to be an amateur technique, but I suspect now more amateurs utilize it than researchers, given the advent of new...

Break out you camera manual. Somewhere in there is a function to allow shutter release without a lens. Enable it. It's disabled by default since normally you take pictures with a lens. Obviously your adapter doesn't tell the camera it is a lens. Also see: https://www.dpreview.com/forums/thread/4437376

Years ago, my first microbiology professor said of heat fixing, to pass it through the flame 2-3 times, smear up, then lay the slide on the back of your (then ungloved) hand. If you heard the sound of sizzling flesh, it was too hot!

In clinical labs dry mounted blood smears get a thin coating of immersion oil over them prior to examination with low power objectives to reduce diffraction. Horrible images otherwise. Body fluid smears and grams stains, too. It acts just like a coverslip. Another drop is added when going up to 100x...

Nothing. The tissue or blood smear remains fixed to the glass as long as you don't accidentally scrape part of it off! I can't vouch for specimens not originally fixed to the glass slide.

Probably Dero digitata (Ward and Whipple, Fresh Water Biology, 2nd. Ed., pgs 530-531, and https://worms.myspecies.info/taxonomy/term/936)

Edit: Nice images, too!

Yes. Before I retired I re-coverslipped a couple hundred teaching slides I had made as a student and mounted with Permount. Soak in Xylene if you can. It's a lot faster than the citrus-based solvents. They work, they're just slower. Xylene should loosen the coverslip in a couple of days. Flood the s...

Be aware that video usually does not use the full sensor. :cry: Look in your camera specs to see the pixel dimensions and you can calculate the physical size of the part of the sensor being used for the resolution you have chosen. Each of the various video formats will use a different set of pixel d...

Have you checked your camera settings to be sure your photos are not being saved as a really low resolution jpg?

Just don't use it in clinical medicine in the US as it's legally a medical device and needs FDA 510(K) clearance before it can be used.

Thanks Wes!

It's not that these posts aren't annoying. They certainly are. But requiring an answer to a rather esoteric question doesn't seem like a productive way to screen potential members for bots. They're usually pretty obvious, and I don't find them annoying enough to do anything keep new folks away. This...

Wes wrote: How about "calculate the resolution of a microscope with a 63/1,4 objective coupled to a 0.9 condenser at 550 nm"? I'd venture a guess that most amateurs wouldn't care what the resolution is numerically. Most professional microscope users wouldn't either. I know I've never bothered to cal...

Have you tried running your old software in the Win 10 Compatibility Mode? It's designed to allow older software to run on Win 10.

It's generally not important. For a little perspective, in clinical labs we used a LOT of oil. Every single WBC differential slide, and every single gram stain, was examined under oil immersion at the objective only. Unless the slide had be coverslipped and stored for later pathology review, we didn...

That's an absolute steal. If I was anywhere near you I'd grab it, and I already have one!

Have a chat with your university's head of clinical or epidemiological microbiology. They have all the resources you will need.
If you want to do research at a university medical school, you need to go through the proper channels.

No offense, but I think you need to go back and reassess what you want to do, and why. You say you want to seen protein and collagen, but some pretty simple things like phase contrast and polarization are a bit over your head. To be brutally honest, until you can be a lot more specific about your qu...

Nicely done! I appreciate the labels, too. They're quite a bit of work, so most people don't use them.

Tom

My Olympus BHS system produces nice dark backgrounds as well as the full range of colors depending on how far the analyzer prism is translated. On the other hand, my Olympus BX system with the DICT slider will not produce a good darkfield effect with or without the 530nm waveplate. Only the full ran...

microscopeboi, For a little bit of introductory homework, see: https://en.wikipedia.org/wiki/Differential_interference_contrast_microscopy https://www.olympus-lifescience.com/en/microscope-resource/primer/techniques/dic/dicintro/ https://en.wikipedia.org/wiki/Waveplate https://www.youtube.com/watch?...

Thanks Michael!

Anything you output to a 720p system will be limited to 720. The original resolution doesn't matter. Even 8K to 720 still gives 720, and that's pretty low-res when blown up to projection scale. 720 looks pretty good on a laptop screen. If you double the size vertically and horizontally (essentially ...

I used "Weld-On 4SC".
On Amazon: https://www.amazon.com/SCIGRIP-Adhesive ... ast_sto_dp

ewieckow, As you requested, here are the two I use. The first is a custom one I built out of 1/4" ABS plastic glued together, and 1" urethane foam (I think). The foam is pretty stiff and is glued in place with 3M 77 adhesive. Any slots or holes needed, like for the lamp house, were routed out. Notic...

What woyjwjl said: From the price/performance point, the M43 camera (Panasonic GH4) + NFK3.3 + U-PMTVC adapter (catalog number U-V210, 037151) provides perfect access to 4K images and videos. That combination, along with DIC on my BHS, is what I used to shoot the 2018 Nikon Small World in Motion Hon...

Your D3200 is limited to Full HD per Nikon's website, 4K is not possible. You'll need a camera that will output 4K video to clean HDMI. Your computer screen will need to be 4K as well. That's not happening with an old laptop. Your cam-link clone is probably not 4K either. 4K takes a high-throughput ...

Don't try to carry it all the time. It's too big and too awkward. With that much weight in front of you, it's hard on your back, and stairs will be very dangerous. You will get hurt. I have seven BHTU's, two of which are trinocs, that I transport for outreach events. Six are fitted upright, in custo...

Pasteur pipettes are a little difficult to use with such small volumes. Capillary tubes are better for loading the chambers. Cheaper, too. The thicker cover slips are heavier and less prone to float on top of the specimen if it's viscous. Otherwise, either one works fine. Unless you get one with the...

Wow.