Search found 84 matches
- Fri Mar 22, 2024 11:56 pm
- Forum: Microscopes and optics
- Topic: who has picture of this Reichert objective?
- Replies: 3
- Views: 1037
Re: who has picture of this Reichert objective?
Reichert changed the lettering format a few times. There is this one you may have already seen. Not exact and not great pictures. https://www.ebay.at/itm/363916493207?itmmeta=01HSKQ1RCV42YQWV0A6CK0WYA3&hash=item54bb1d0197:g:3MsAAOSw~m1i2SkR&itmprp=enc%3AAQAJAAAA4HLOI1NbIitnVtYcXgilgOq0CNur%2FSWhVyn...
- Fri Mar 22, 2024 3:14 pm
- Forum: Illumination Techniques
- Topic: question about HMC: why do we need both modulator and slit?
- Replies: 1
- Views: 954
Re: question about HMC: why do we need both modulator and slit?
And the weird thing is, when the slit is mapped to modulator, the order of bright, gray is opposite to each other. Is this specially designed?
- Fri Mar 22, 2024 3:12 pm
- Forum: Illumination Techniques
- Topic: question about HMC: why do we need both modulator and slit?
- Replies: 1
- Views: 954
question about HMC: why do we need both modulator and slit?
I've been reading articles about HMC, for me the slit and modulator both create gradient pattern applying to the image, and they are conjugate to each other any way, why do we need both?
- Fri Mar 22, 2024 2:35 pm
- Forum: Microscopes and optics
- Topic: who has picture of this Reichert objective?
- Replies: 3
- Views: 1037
who has picture of this Reichert objective?
I have a 10x Reichert objective, the marks are almost gone.
I searched on google and bing, can not find the exact the same picture.
Is there a place to find pictures of objectives?
I searched on google and bing, can not find the exact the same picture.
Is there a place to find pictures of objectives?
- Sat Mar 02, 2024 3:05 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
I read the posts and watched the video, it is quite impressive! Do you have pictures of diatom or other stuff? I think oblique illumination can pretty much improve resolution and contrast.
- Sat Mar 02, 2024 3:03 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
Your filter cutting looks o.k. to me but in these types of lighting adaptions geometry and materials selection are as important as precision. I am wondering if you have tried varying the slit width much and used rotated assymetry ? It seems that in some ways you are making a modified diy version of...
- Sat Mar 02, 2024 2:27 pm
- Forum: Microscopes and optics
- Topic: has anyone used Leica S-APO 10x objective?
- Replies: 9
- Views: 1045
Re: has anyone used Leica S-APO 10x objective?
The 506289 objective was never a derivative of the Reichert Plan Fluor objective family. They came from two different factories, had different reference focal lengths and did not share optical design . The PL S Apo 10x objective has a 11mm working distance as compared to other Apo 10x. This is why ...
- Fri Mar 01, 2024 8:24 am
- Forum: Microscopes and optics
- Topic: has anyone used Leica S-APO 10x objective?
- Replies: 9
- Views: 1045
Re: has anyone used Leica S-APO 10x objective?
Thanks, I'll skip this one then. It's around $240 though.
- Fri Mar 01, 2024 1:21 am
- Forum: Microscopes and optics
- Topic: has anyone used Leica S-APO 10x objective?
- Replies: 9
- Views: 1045
has anyone used Leica S-APO 10x objective?
Hi, all There is a Leica HCX PL S-APO 10x0.30 on the market, ID 506289. However I tried to search in Leica website and google, there is no such record, only a paper. https://bio-protocol.org/exchange/minidetail?id=10271842&type=30 I guess this S-APO is not really the APO, maybe specially designed fo...
- Wed Feb 28, 2024 2:30 pm
- Forum: Microscopy accessories
- Topic: anyone knows what this lense is for?
- Replies: 2
- Views: 1002
Re: anyone knows what this lense is for?
There are a number of reasons for a lens or 2 to be in the photo tube, depending on the original desgn of the microscope. When the tube is mfg. specifically to receive a certain camera or sensor size, the lens could be a framing lens of a certain magnification , so that the projection matches the s...
- Wed Feb 28, 2024 10:01 am
- Forum: Microscopy accessories
- Topic: anyone knows what this lense is for?
- Replies: 2
- Views: 1002
anyone knows what this lense is for?
I bought a trinocular part ( sales say this is from Motic BA600 ), however, one lens is missing Weixin Image_20240228174430.jpg If I mount a camera on it, the image is quite blur, appears to be kind of tube lens is missing. Then I took a look at the trinocular, there is a convex lens at the bottom. ...
- Wed Feb 28, 2024 9:24 am
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
It may be beneficial in theory, to have a completely opaque mask blocking 3/4 view, and then split the rest 1/4 into two equal parts of 1/8 each: part #1 = 1/8 blocked by tunable pol filter, and the outside most part #2 = 1/8 completely open to light However, each objective would need a precisely m...
- Wed Feb 28, 2024 12:43 am
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
From the diatom overview photo, I am guessing your dark center stop is slightly too big than optimal (that is why center of the photo is much darker). Were your dark center mask about the same size as the pol center stop? If they were of the same size and both being slightly too big, then I agree t...
- Tue Feb 27, 2024 12:54 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
If we zoom further in, we will see the depth of view with polarized filter is much less than dark stop.
- Tue Feb 27, 2024 12:37 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
Nice work. Have you compared: 1) COL with a totally opaque (totally black) central mask + polarized peripheral light outside of the central mask with This is the comparison: both allows narrow light at the fringe, left side polarized filter, right dark stop, 100% diatom with polarized filter vs dar...
- Mon Feb 26, 2024 2:49 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
This is the diatom with polarized filter
- Mon Feb 26, 2024 2:43 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
Re: COL with polarized filters
This is how it looks like. The there is a fixed angle between the two filters so that the light through the two of them can be adjusted simultaneously.
- Mon Feb 26, 2024 2:37 pm
- Forum: Home-made microscope adaptations
- Topic: COL with polarized filters
- Replies: 15
- Views: 2856
COL with polarized filters
From some articles I learned COL could bring good contrast and resolution, but the stop in the middle is just black and white. Then I made a polarized filter to act as a stop, which is adjustable.
Here is the effect without and with filter: ( flower pistil cut )
Here is the effect without and with filter: ( flower pistil cut )
- Mon Feb 26, 2024 6:36 am
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
Re: How do you deal with the glare in camera
This is how it looks
- Mon Feb 26, 2024 6:28 am
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
Re: How do you deal with the glare in camera
Hi all I finally found a solution. It turned out to be the reflection in the adaptor tube. 3D printed adaptor ( ABS ) naturally brings some reflection. I tried to use other black material, like black paint for car, dark ink, etc., none of them helps. The ultimate solution is light-absorbing matte fl...
- Mon Feb 26, 2024 2:59 am
- Forum: Microscopes and optics
- Topic: Is it ok to use metalloscopy objective in biology microscope?
- Replies: 8
- Views: 1106
Re: Is it ok to use metalloscopy objective in biology microscope?
Thanks for all the reply and help. The motivation of this idea is to buy a cheap metalloscopy objective for multiple purpose. However, the focal length is an issue, it's way too long ( 95mm ). Besides the one I tried to buy is tainted with fog, I gave it up. Still I want to use such objective on a b...
- Tue Feb 20, 2024 8:16 am
- Forum: Microscopes and optics
- Topic: Is it ok to use metalloscopy objective in biology microscope?
- Replies: 8
- Views: 1106
- Tue Feb 20, 2024 7:01 am
- Forum: Microscopes and optics
- Topic: Is it ok to use metalloscopy objective in biology microscope?
- Replies: 8
- Views: 1106
Is it ok to use metalloscopy objective in biology microscope?
I need a good 10x APO objective in my Motic biology microscope, but sometimes I also use it to take pictures of small things like mosquito. There are some quite cheap metalloscopy APO objectives on the market, has anyone used it in biology cases like diatom? Is the APO image quality compromised? I n...
- Wed Feb 07, 2024 3:16 pm
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
Re: How do you deal with the glare in camera
A couple of thoughts: Check your kohler alignment and make sure the field stop is opened sufficiently. This looks like a "hot spot" from a mis-aligned condenser. Kohler alignment can change with cameras, as the adaptors (or lack thereof) that you add to the optical path can change the focal length ...
- Wed Feb 07, 2024 12:47 pm
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
Re: How do you deal with the glare in camera
This is how this camera looks like. I suspect it's because the filter in front of the CMOS sensor caused the problem, probably there is no anti-reflective coating.
Do you see similar issue?
Do you see similar issue?
- Wed Feb 07, 2024 12:44 pm
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
Re: How do you deal with the glare in camera
Left: camera with adaptor Right: camera without adaptor
- Wed Feb 07, 2024 12:40 pm
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2483
How do you deal with the glare in camera
Every time I use this industrial camera I see glare.
Left side: when there is an adapter; Right when the camera directly sit on camera port.
Left side: when there is an adapter; Right when the camera directly sit on camera port.
- Sat Feb 03, 2024 2:04 am
- Forum: Illumination Techniques
- Topic: Has anyone ever used LWD condenser?
- Replies: 8
- Views: 1817
Re: Has anyone ever used LWD condenser?
I think tis probably gives the game away: https://www.fishersci.fi/shop/products/condenser-achromatic-lwd-ba410/15425302 The long working distance facilitates its use with phase contrast accessories MichaelG. . See also: https://moticeurope.com/en/lwd-achromatic-condenser-na-065-wd108mm-with-iris-d...
- Fri Feb 02, 2024 3:51 pm
- Forum: Illumination Techniques
- Topic: Has anyone ever used LWD condenser?
- Replies: 8
- Views: 1817
Has anyone ever used LWD condenser?
I came across a Motic LWD Achromat condenser, and compared it with normal abbe condensers. https://moticeurope.com/media/catalog/product/cache/ef0b392b87b09aac7ddf3931bf54d740/1/1/1101001901261_lwd_achromatic_condenser_na_0dot65_wd_10dot8mm_with_iris_diaphragm_00.jpg However, its NA is quite low, wh...
- Mon Jan 29, 2024 2:24 pm
- Forum: Beginner's corner
- Topic: How do you clean the surface of objective
- Replies: 8
- Views: 1777
Re: How do you clean the surface of objective
It already looks 80% improved. Quite deftly done. I have used a flat sided toothpick ( not the round pointed ones) around the paint to apply a bit of metal polish here and there, and a clean one to buff it. Lightly wipe any residue with ipa. Bic and probably others make a " Sharpie Pen" with a very...