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And this the picture best matches what I see through microscope.
https://www.tandfonline.com/doi/pdf/10. ... ccess=true

Could be that the left image is a top view in post #1 (the valves lie horizontally) and the right image is a girdle view (the valves lie vertically) of the same diatom ? Thanks Hobbyst46, The picture below shows the same diatom looking with different focal depth, where we can see it's like a transp...

Thanks to Google, Today I searched the picture and ended up with a name : Thalassiosira eccentrica



This shows it's really a cake-shaped diatom that I see from aside, and we can see some flagellum.

Hobbyst46 wrote: ↑

Sun May 05, 2024 11:25 am

Could be that the left image is a top view in post #1 (the valves lie horizontally) and the right image is a girdle view (the valves lie vertically) of the same diatom ?

Thanks for the reply. I thought so, but clearly the right image got flagellum, that's why I suspect it's another diatom.

Fantastic video!

The lefthand side microbe looks quite cyclotella, the righthand side looks like crabs with four hair. This is the source I found: https://outerhebridesalgae.uk/diatoms/diatom-species.php?id=507 https://outerhebridesalgae.uk/images/species/diatoms/Cyclotella%20meneghiniana%201.jpg http://protist.i.ho...

Hi, all

I found some different microbes in a drop of pond water, and search on the internet, found these are all Cyclotella meneghiniana. For me these look quite different:

Reichert changed the lettering format a few times. There is this one you may have already seen. Not exact and not great pictures. https://www.ebay.at/itm/363916493207?itmmeta=01HSKQ1RCV42YQWV0A6CK0WYA3&hash=item54bb1d0197:g:3MsAAOSw~m1i2SkR&itmprp=enc%3AAQAJAAAA4HLOI1NbIitnVtYcXgilgOq0CNur%2FSWhVyn...

And the weird thing is, when the slit is mapped to modulator, the order of bright, gray is opposite to each other. Is this specially designed?

I've been reading articles about HMC, for me the slit and modulator both create gradient pattern applying to the image, and they are conjugate to each other any way, why do we need both?

I have a 10x Reichert objective, the marks are almost gone.

I searched on google and bing, can not find the exact the same picture.

Is there a place to find pictures of objectives?

Saul wrote: ↑

Wed Feb 28, 2024 7:42 pm

https://www.photomacrography.net/forum/ ... 12#p269212

I read the posts and watched the video, it is quite impressive! Do you have pictures of diatom or other stuff? I think oblique illumination can pretty much improve resolution and contrast.

Your filter cutting looks o.k. to me but in these types of lighting adaptions geometry and materials selection are as important as precision. I am wondering if you have tried varying the slit width much and used rotated assymetry ? It seems that in some ways you are making a modified diy version of...

The 506289 objective was never a derivative of the Reichert Plan Fluor objective family. They came from two different factories, had different reference focal lengths and did not share optical design . The PL S Apo 10x objective has a 11mm working distance as compared to other Apo 10x. This is why ...

Thanks, I'll skip this one then. It's around $240 though.

Hi, all There is a Leica HCX PL S-APO 10x0.30 on the market, ID 506289. However I tried to search in Leica website and google, there is no such record, only a paper. https://bio-protocol.org/exchange/minidetail?id=10271842&type=30 I guess this S-APO is not really the APO, maybe specially designed fo...

There are a number of reasons for a lens or 2 to be in the photo tube, depending on the original desgn of the microscope. When the tube is mfg. specifically to receive a certain camera or sensor size, the lens could be a framing lens of a certain magnification , so that the projection matches the s...

I bought a trinocular part ( sales say this is from Motic BA600 ), however, one lens is missing Weixin Image_20240228174430.jpg If I mount a camera on it, the image is quite blur, appears to be kind of tube lens is missing. Then I took a look at the trinocular, there is a convex lens at the bottom. ...

It may be beneficial in theory, to have a completely opaque mask blocking 3/4 view, and then split the rest 1/4 into two equal parts of 1/8 each: part #1 = 1/8 blocked by tunable pol filter, and the outside most part #2 = 1/8 completely open to light However, each objective would need a precisely m...

From the diatom overview photo, I am guessing your dark center stop is slightly too big than optimal (that is why center of the photo is much darker). Were your dark center mask about the same size as the pol center stop? If they were of the same size and both being slightly too big, then I agree t...

If we zoom further in, we will see the depth of view with polarized filter is much less than dark stop.

Nice work. Have you compared: 1) COL with a totally opaque (totally black) central mask + polarized peripheral light outside of the central mask with This is the comparison: both allows narrow light at the fringe, left side polarized filter, right dark stop, 100% diatom with polarized filter vs dar...

This is the diatom with polarized filter

This is how it looks like. The there is a fixed angle between the two filters so that the light through the two of them can be adjusted simultaneously.

From some articles I learned COL could bring good contrast and resolution, but the stop in the middle is just black and white. Then I made a polarized filter to act as a stop, which is adjustable.

Here is the effect without and with filter: ( flower pistil cut )

This is how it looks

Hi all I finally found a solution. It turned out to be the reflection in the adaptor tube. 3D printed adaptor ( ABS ) naturally brings some reflection. I tried to use other black material, like black paint for car, dark ink, etc., none of them helps. The ultimate solution is light-absorbing matte fl...

Thanks for all the reply and help. The motivation of this idea is to buy a cheap metalloscopy objective for multiple purpose. However, the focal length is an issue, it's way too long ( 95mm ). Besides the one I tried to buy is tainted with fog, I gave it up. Still I want to use such objective on a b...

Alexander wrote: ↑

Tue Feb 20, 2024 8:02 am

Metallurgical objectives work in transient light as fine as in reflected liegt.

The question is: fits the objective the system.

There are some M26 to RMS adapters on the market, it should not be a problem. My concern is more on the side of coverslip.

I need a good 10x APO objective in my Motic biology microscope, but sometimes I also use it to take pictures of small things like mosquito. There are some quite cheap metalloscopy APO objectives on the market, has anyone used it in biology cases like diatom? Is the APO image quality compromised? I n...