MicrobeHunter.com Microscopy Forum

[/quote]@Nubee-70 : IMHO, although the electronic project is probably a lot of fun to construct, the variety of useful wavelengths (namely, colors) for microscopy is quite limited - except, maybe, for excitation in fluorescence microscopy. For the latter, UV illumination can be useful, but precautio...

… I think, still, that the OPs project will still offer some interesting results … Most definitely … I’m looking forward to seeing them MichaelG. Gee fellows, I don't know if there will be any interesting results. I am a complete beginner in microscopy and a lot of the stuff you guys talk about go ...

The subject gets a bit heavy, Gerry … but this is a good introduction: https://www.animations.physics.unsw.edu.au/jw/light/colour-mixing.htm The visual perception of a particular colour does not necessarily mean that colour’s actual wavelength is present. MichaelG. Thank you Michael for sharing the...

MichaelG. wrote: ↑

Tue Jan 02, 2024 9:03 am

...
In an ideal world, however, you would polish those sawn faces and get total internal reflection
[ big trade-off in the realm of reward-for-effort though ]
MichaelG.

Thanks.
I will clean it up to the point where I know that further polishing becomes inefficiently effective.

I believe there's a flaw in your reasoning here. Combining light from two or three differently colored LEDs does _not_ generate light of any color. You can get light that appears to your eyes as any color you like, but the actual physical characteristics of that light are limited to the 20 nm or so...

While waiting for electronic parts, I went ahead with fashioning a clear acrylic rod (20mm dia) to a point of about 5mm dia. At the moment, it is just a rough job to see who it would work as a means to funnel the LED light to a small stage light. I used a hacksaw to make a rectangular frustum with a...

Javier wrote: ↑

Sun Dec 31, 2023 10:23 pm

The secret ingredient!

Dennis wrote: ↑

Fri Dec 29, 2023 8:27 pm

Nubee-70,
Everybody loves mint !

Yeah, but was the grub green or did it become green from eating the mint.
If so, do like green men live on mint?

The schematic diagram has been updated to version 1.4.
Two sockets and plugs are shown, both are six pin.
The IR and UV probe can be operated/selected in the following ways: IR1. IR1+IR2. UV1. UV1+UV2. IR1+UV1. IR1+IR2+UV1+UV2.

Thanks GerryR for your shae about similar ideas. Interesting. Currently, with this project, I will be using two flexible goosenecks with multi-pin socket in its end. The probe end, of various designs, can then be selected and plugged in to it. RE: Optic fibres. I used to scratch build Sci-Fi models...

It is interesting that I was thinking about a similar project. My solution was using a 555 astable at ~100hz and triggering four more 555s set up as one-shots with a variable period to regulate the intensity of the four LEDs, R, G, B, Y (poor mans PWM). Because they are all triggered at the same ti...

After three attempts I think I found a working circuit, but I will soon find out after a few parts are posted to me. Then I will put them onto the breadboard to see if the circuit actually works. And after fine tuning it, I will finally know the component values. I already have most of the parts, bu...

My substitute low powered stereo microscope is my iPhone with a 5x zoom.

After my bicycle ride I often enjoy having a banana mint milkshake.
Today I found something else that likes my mint too.

A few goosenecks were delivered from EBay. At least two of them will be incorporated into the set-up for extra stage illumination.
See the project at viewtopic.php?f=12&p=140610#p140583

Why use PWM rather than a constant current driver? So I can alter the degree of chromicity between the two coloured LEDs; offering a variable colour selection between the two without altering the degree of required illumination. I'd think you could do the same with constant current since they can v...

I will try again :oops: MichaelG. https://www.adafruit.com/category/168 Cool 8-) Thanks for the link. I will be buying a few LEDs from them to play with. But at the moment need to see my idea through. Perhaps I will be ending up making various LED applications as I gain more knowledge and resources.

There will be some issues with properly collimating two LEDS - and then the results might not be all that interesting. Other users have swapped in IR and UV sources - but each was positioned centrally to match the old tungsten filament position. Some other possibilities you might want to try: 1) Il...

MichaelG. wrote: ↑

Wed Dec 27, 2023 7:38 pm

It may be worth exploring these: /https://www.adafruit.com/category/168url
.
Ada deserves a lot of credit for her development work, but I should mention that even-cheaper ‘clones’ are to be found on ebay.

MichaelG.

Sorry, but the link does not go to what you want to show me.

Scarodactyl wrote: ↑

Wed Dec 27, 2023 7:21 pm

Why use PWM rather than a constant current driver?

So I can alter the degree of chromicity between the two coloured LEDs; offering a variable colour selection between the two without altering the degree of required illumination.

It might help to know which type of microscope illumination is wished. Both brightfield and fluorescence, at least, benefit from a single die LED source, rather than a multi-LED arrangement. Besides, I wonder about the wavelength combination... For visual observation, green or blue or amber light a...

I have been thinking about how to blend two coloured LEDs to make a composite colour, such as red and blue to make purple. If I mixed any two primary coloured LEDs (red, yellow, green, blue, and white) then I ought to be able to make almost any colour I want. To do this, I figure that each LED ought...

. In the immortal words sung by Julie Andrews: … a very good place to start. I wish I could be so meticulous and thorough. MichaelG. Thanks MichaelG. I too once thought the same thing about others being meticulous and thorough. Then over the years of errors in my work I got more cautious and done m...

apochronaut wrote: ↑

Wed Dec 27, 2023 6:44 am

That is nice straight forward microscope. It reminds me of a Korean microscope I saw a few years back. More than a hobby scope.
Your system seems well planned. Good luck and have good microscopy.

Thank you apochroaut.

I wish to share my Work In Progress (WIP) microscope set-up. Firstly, at the moment, I do not have the home space or room to set-up a mini lab. So, one end of my work table will have to do. Secondly, my spending money is limited. The microscope I have will just have to do. In other words, buying oth...

These are great! Pity we can't add a 'like' to them.

Pity I cannot see the image to get a better idea of what you are describing.

I think you have made an excellent start with the new microscope … methodical tests, using an easily recognisable specimen * Well done ! . As you evidently have an iPhone, my tip would be to grab these two very useful Apps: https://apps.apple.com/gb/app/koehler-tutor/id1281328178 https://apps.apple...

From what I have just been reading, I believe the previous post pictures show that I have bungled the images up. Especially by the last two images of 400x and 1000x. I just read that I need to adjust both the iris diaphram and condensor position after each time I select a different objective. Later,...

Decided to do a simple comparison between the four objectives using the small print of an email on A4 copy paper.



How did it turn out?

It finally came for Xmas. Received the Optico XSZ-107T Trinocular microscope through the post. Everything very well packed and arrived safely. It has the following: WF10x/20 High Eyepoint Eyepieces 23mm (Pair). Infinity-plan objectives set 4x, 10x, 40x, and 100x. Optico GT5 - 5 Mp digital microscope...