MicrobeHunter.com Microscopy Forum

That's a horrible experience apo, sorry man. I didn't know the seller would pay for returns. The seller of this objective sent me that picture on request since the image in the listing was blurry. Seller also stated via messaging that "it's all good" and he would accept returns, but this is not stat...

Thanks for the input guys. There seller accepts returns, but with $100 shipping both ways I'll pass on this one. My 100x is in good condition, and I do have a phase slider for it. One thing I've found though is that I prefer staining specimens instead of using phase contrast, and comparing a 40x pha...

I came across an affordable Leica 100x/1.30 PL Fluotar, but the front has a lot of scratch marks from collisions. What are the chances that the optics are compromised? I don't think this objective has a spring loaded core. Another question is whether this is a good upgrade from my 100x C Plan PH3. I...

Maybe not a lot of help, but … this appears to feature one in the appropriate setting https://americanlaboratorytrading.com/lab-equipment-products/leica-dm-irb-inverted-research-microscope-14736 MichaelG. . . Edit : __ :geek: __ Which quickly led me to find this https://mmcri.org/wp-content/uploads...

Ah, I see. My plan is to cannibalize an lcd to get a thin plastic polarizer for the slit, and just place a 55mm linear (camera) polarizer on top of the lamp housing. I also have a blue filter on top of the lamp, and at some point I'll probably print a holder for these filters. Having the rotation an...

I appreciate the offer, but I think I'm close to getting where I want with this setup. I'm pretty self sufficient when it comes to DIY, machining and whatnot. I've been thinking I could adapt other condensers to the DMLS, like achromat aplanatic or darkfield condensers, without paying Leica prices. ...

Are you able to view the slit in a bertrand lens? I was going to suggest using the jaws of a vernier caliper as a slit mock up or a small straight jawed clamp, such as a small Jögensen clamp, then transfer the dimensions to a filter disc. Thats a good idea, thanks! Taping up a filter disk was very ...

I made a quick test, making a slit out of aluminium foil pieces. I like this contrast technique a lot, and I'm sure I can get it better than this if manage to adjust the slit size and position with some precision. Here are some tests on a mushroom sample, stained with methylene blue and mounted in K...

I haven't been at this for long, I got the scope in December and the petri dish project took place during the past 2 months. I'd like to use the scope for all things mycology, such as identifying macroscopically similar species, single spore/single cell transfers, and cross breeding of strains. So l...

Cleaned perhaps, but can they take 121C sterilization?

As a reference point. The 506135 is a LMC objective. possessing the following specs. N Plan L 40x/0.55 CORR 0-2, FWD 3.9 -1.9, with M25 threads All CORR objectives from Leica are referred to as Contrast Automatic. This means you can adjust the correction collar until the contrast is best acheived a...

The problem with condenser WD I was thinking of is that standard petri dishes are 16mm high. When I flip a dish upside down, the condenser focus is something like 12-13 mm away from the bottom of the dish. Here's how it looks when I look through the bottom: IMG_3447.jpg This 10x has a WD of 18mm, so...

Thanks all for the input. The catalog number is 506135. I've had a hard time finding info on older Leica parts, and even the current Leica website is sparse with details on their current offerings. I bought this for looking through agar petri dishes, so upright scope should be fine I thought. I didn...

It doesn't move with the correction collar. I think you're right apochronaut, this looks like it: https://www.leica-microsystems.com/science-lab/microscopy-basics/integrated-modulation-contrast-imc/ https://www.mikroskopie-forum.de/index.php?topic=41806.0 I wonder if I can diy a slit/polarizer to ta...

I got this objective for a reasonable $300: Leica N Plan L 40X/0.55 CORR When I look throught the objective, there are two dark areas, one darker than the other as seen here 20240416-AA1_7323.jpg Is this a feature, or something inside the assembly that has been dislodged? I can get a sharp image, al...

I entered microscopy with a similar purpose, although I grew up in a more mycophobic environment in Norway. Anything but chantarelle was considered potentially deadly, and after Chernobyl our family just stopped picking. I've been foraging for the past 5-6 years, also growing various mushrooms indoo...