MicrobeHunter.com Microscopy Forum

This is the version I used and tweaked a bit, a version of 2023. A PDF and some code is downloadable from here: http://www.ipol.im/pub/art/2023/443/ According to what I understood, Ser Player or SharpCap do drop the blue channel and rebuild it from red and green channels. That other software/solutio...

Since you are posting images from a camera mounted on a trinocular, is the same level and spread of ca, evident through the eyepieces? Our eyes and brains are magic. They are able to accommodate and to compensate a lot, lack of depth of field, and high dynamics also... In the eyepieces, the aberrat...

but was I was talking about just shifts around the color channels to reduce some forms of chromatic aberration Their shift around solution seems clear to me. They drop all the blue channel, and rebuild an alternate using the red and green channels. In my setup, I feel I get two spectrum of aberrati...

Those micrometer images . Are they a center crop or if not can you provide a dead center micrometer image? The micrometer image was a crop. The 4k png file is too large to be stored here, downsized to 2k, seems it gets reduced here... Maybe, get a bigger image via X: https://pbs.twimg.com/media/GH3...

What microscope is this and how are the pictures being taken? Cell phone through an eyepiece? My pictures? A BA310e trino with stock Motic EC lenses. I upgraded it with an achromatic Kohler condenser. Camera is a BRESSER MikroCamII 9MP 4K 1'', for its global shutter. Mostly running at 30 (4k) to 60...

SerPlayer... I see not not much info about it using Google. Brought me to SharpCap, a solution embedding it. I'm starting to watch this video. Seems they exclude part of the color spectrum, to rebuild colorful pictures without aberrations, with a "synthetic blue channel", "preventing defocused blue ...

Yes, I noticed RegiStax, designed for astronomy, to compensate waves due to atmospheric changing conditions. I didn't try this, as I'm not convinced it could help on such bad images, green/yellow and more fringes being often very present, depending on contrast and magnification. Seems also inappropr...

Hello, I use achromatic objectives, and I'm often disappointed by pictures I get from them, especially at 40x and 60x. How do you deal with them, and without switching to costly fluor or apochromatic objectives? I found some software, by image processing experts: https://github.com/teboli/chromatic_...

1) Is focus stacking from video any easier, faster (or harder) than from stills? 2) Is it any better in terms of quality? 3) I see no real advantages... 4) Motor vibration is a real problem for macroscopy....imaging how much worse it is at 200x or even 400x! I do focus stack also live beasts, using...

Hi, our host explained who they are, and that we all are crowded

my subjects are mostly alive and less-than-optimally prepared which augments the focus problem for me. But seeing your first image, with nothing or not much in focus, I think you could have an issue with your setup. Aren't the images better in your eyepiece? This is a video I took, of a live big wo...

Hi, I've a photo port to c-mount adapter, which came with a 5mm extension ring. I used it that way for some days, but my pictures were all disapointing. I was then told to remove this extension ring, which is supposed to be used with specific cameras, maybe with cs-mount cameras (CCD not at the same...

Hi all, I went from a €200 microscope to a bit more, but still entry level achro objectives. For fun, and often amazed by what I'm looking at, whatever it is. Images being stored on X, you may need an account there to see them... https://pbs.twimg.com/media/GGK0HHQWYAA97I4?format=jpg&name=large http...