Search found 1604 matches

by PeteM
Mon Apr 19, 2021 2:49 am
Forum: Miscellaneous
Topic: microspectrophotometry
Replies: 1
Views: 47

Re: microspectrophotometry

You might find a surplus scope from the semiconductor industry, used to find "hot spots" on chips and boards as a stand or chassis on the scope end of things. These typically used reflected objectives (sort of like telescope mirrors) to avoid reducing the IR signal too much. And then a camera sensor...
by PeteM
Sun Apr 18, 2021 8:00 pm
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 4
Views: 103

Re: should i buy Bresser phase contrast series 57?

Bresser, like AmScope and many others, puts their brand on generic Chinese scopes. Find a picture of the Bresser 57 model you're considering and you might find reviews of essentially the same microscope, out of the same factory under another brand name. I have mixed feelings about the $1000 or so Ch...
by PeteM
Sun Apr 18, 2021 6:35 am
Forum: Microscopes and optics
Topic: AO 1240 phase condenser (for model 10) on 110
Replies: 11
Views: 172

Re: AO 1240 phase condenser (for model 10) on 110

There is just enough metal in the older too-tight phase condenser to open up the width to get a fit from series 10 through 110 and 410. That will require a metal lathe and a tool able to reach into the recess. You can also reduce the thickness of the condenser holder "fork" on the target microscope ...
by PeteM
Fri Apr 16, 2021 3:48 am
Forum: Beginner's corner
Topic: Did I make a mistake? AmScope T120?
Replies: 5
Views: 214

Re: Did I make a mistake? AmScope T120?

A modern cell phone camera takes surprisingly good photos. Some models will have USB-c or Bluetooth output to monitors. Most will take decent pictures and movies. The trick to getting them to work is to buy a $15 cell phone holder meant for microscopes and telescopes. If you happen to have a previou...
by PeteM
Thu Apr 15, 2021 12:46 am
Forum: Microscopes and optics
Topic: Nikon Plan objective - washed out colours
Replies: 12
Views: 278

Re: Nikon Plan objective - washed out colours

Nikon "CFN" plan achromat finite objectives are equivalent to Olympus S Plan and available used at around the same prices. Ditto the plan apo. These have three heavy knurled rings on the chrome color body and slightly higher numerical apertures than Nikon's lesser plan achromats in all but the 100x....
by PeteM
Sun Apr 11, 2021 8:31 pm
Forum: Camera systems and imaging
Topic: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography
Replies: 3
Views: 174

Re: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography

That looks to be a very good result. Thanks for sharing it.

Do the Zeiss KPL 8x lower half and 10x upper half simply screw together for the spacing you used?
by PeteM
Sat Apr 10, 2021 7:17 pm
Forum: My microscope
Topic: My Microscopes and Amscope Purchases
Replies: 8
Views: 620

Re: My Microscopes and Amscope Purchases

[/quote] . . . As for the halogen, it will kill stuff and dry out your slides. However the slide has to be over the light for about an hour, maybe an hour and a half before this starts happening. So it's not really a huge issue. I find it's about that time I am ready for another drop of water or wha...
by PeteM
Sat Apr 10, 2021 6:39 pm
Forum: Illumination Techniques
Topic: can Phase contrast kit work WITHOUT Köhler ???
Replies: 17
Views: 544

Re: can Phase contrast kit work WITHOUT Köhler ???

If you don't already have one, you might want to get a centering or phase telescope to adjust things so that the phase annulus and the objective phase ring are coincident - with no light sneaking around your DIY phase rings.
by PeteM
Sat Apr 10, 2021 6:36 pm
Forum: Beginner's corner
Topic: Amscope T340B-DK vs. Amscope T490B-DK
Replies: 19
Views: 447

Re: Amscope T340B-DK vs. Amscope T490B-DK

[/quote] ok, so after some research I've figured out that 3W in LED is equivalent to ~20-25W in halogen. :D [/quote] Various sources suggest that halogen lamps put out around 15-25 lumens per watt - let's say 20. For LED it's around 70-100 lumens per watt - let's say 80. So, LEDS have about 4x the l...
by PeteM
Sat Apr 10, 2021 2:29 pm
Forum: Introduce yourself
Topic: Hello microbehunter friends!
Replies: 8
Views: 366

Re: Hello microbehunter friends!

Welcome.

Making great images is hard work. Keep at it. The results will eventually be beautiful.
by PeteM
Sat Apr 10, 2021 5:20 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 1543

Re: PZO DIC on a BHT microscope, possible?

Synnus - thank you for taking the time to do that comparison.
by PeteM
Sat Apr 10, 2021 1:57 am
Forum: Introduce yourself
Topic: Hi! And some advice if you have any?
Replies: 22
Views: 5926

Re: Hi! And some advice if you have any?

I've recently acquired a Motic 168 and can add a third perspective. Mine seemed well made, but came to me both misaligned and with poor zoom tracking (such that the images were of different sizes at some points while zooming from one magnification to another). The alignment problem was a prism that ...
by PeteM
Sat Apr 10, 2021 1:42 am
Forum: Microscopes and optics
Topic: PZO DIC on a BHT microscope, possible?
Replies: 50
Views: 1543

Re: PZO DIC on a BHT microscope, possible?

I think pzo dic can fit bh2 https://youtu.be/TQbKw3CeZ58 Curious how much of a 3D effect you're getting at various magnifications? The images in the first video show a wide range of "color staining" - which is a feature that DIC prisms can provide when slide to extremes (much like wave plates) but ...
by PeteM
Thu Apr 08, 2021 9:34 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB - Plan objectives for Alphaphot
Replies: 8
Views: 396

Re: WTB - Plan objectives for Alphaphot

There are so many sellers and prices varying at times that I'd suggest you do a current Ebay search. If you currently have the Nikon E Plan with a black paint finish there is a Chinese plan achromat with a similar look available from AmScope, Ken-A-Vision, and various other sellers. If you have brig...
by PeteM
Thu Apr 08, 2021 5:40 am
Forum: My microscope
Topic: Leica DM 2500 LED
Replies: 2
Views: 522

Re: Leica DM 2500 LED

Great scope - and capable of DIC should you want to add it at some point. A few of us may be curious to know what digital photo system you end up with? The Leica apparently does a bit of correction in the eyepieces - and the ideal probably has some sort of relay lens to something like a mirrorless c...
by PeteM
Wed Apr 07, 2021 11:19 pm
Forum: Beginner's corner
Topic: Concerns on SW380T condenser
Replies: 6
Views: 440

Re: Concerns on SW380T condenser

The stage-attached condenser kind of goes with the price point. It will still work way better than the pinhole diaphragms of even cheaper scopes - just not with the easy focusing and multiple condenser options of more expensive or once-expensive used scopes. Similar deals with the cost-reduced illum...
by PeteM
Wed Apr 07, 2021 10:08 pm
Forum: Beginner's corner
Topic: Pls help me spend my money!
Replies: 7
Views: 458

Re: Pls help me spend my money!

Happy to send you the guide we use to various microscope brands and models if you PM where you want a PDF sent. Might help you find a great used $400 scope.
by PeteM
Wed Apr 07, 2021 3:56 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 30
Views: 1267

Re: Slide Making with 61°C Wax

My take is that exceptional skill and a willingness to help others - often at some effort - should be recognized. John and Bob are among those who have my respect and gratitude for their many contributions. I'll often provide links to their comments for kids aspiring to learn more about science star...
by PeteM
Wed Apr 07, 2021 1:56 am
Forum: Specimens, samples and slides
Topic: Using aluminium tape to make spacers for water sample specimens
Replies: 11
Views: 556

Re: Using aluminium tape to make spacers for water sample specimens

The foil tape idea is pretty clever, IMO. Pretty clean mount vs. something like Vaseline, with just a bit of water escaping at first. Could also be easily done to allow adding water from the side to keep a mount fresh (with the gap in the foil maybe mid-side). Provides a consistent and reproducible ...
by PeteM
Wed Apr 07, 2021 1:46 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 30
Views: 1267

Re: Slide Making with 61°C Wax

Super informative post, John. Thank you! Ideally this will be up for years (decades?) to come and a guide for aspiring microscopists.
by PeteM
Mon Apr 05, 2021 11:23 pm
Forum: Microscopes and optics
Topic: Zeiss Photomicroscope DIC Inko questions.
Replies: 7
Views: 357

Re: Zeiss Photomicroscope DIC Inko questions.

Phase objectives rarely work very well with DIC.
by PeteM
Mon Apr 05, 2021 6:46 am
Forum: Miscellaneous
Topic: Ocean Waves Under Polarizar
Replies: 2
Views: 190

Re: Ocean Waves Under Polarizar

Maybe another way to look at it - when we view something like a reflective silicon wafer with polarized light in an epi microscope - the light source is polarized (just like skylight is a polarized light source). Rotate the analyzer just right and the reflected light shows up as blue (or some other ...
by PeteM
Mon Apr 05, 2021 6:22 am
Forum: Miscellaneous
Topic: Ocean Waves Under Polarizar
Replies: 2
Views: 190

Re: Ocean Waves Under Polarizar

Here's one guesstimate, based on too-long-ago memories of physics class. Start with "why is the sky blue" (the full spectrum of sunlight has the shorter blue wavelengths scattered more widely in our atmosphere). So indirect skylight looks blue. The scattering process also polarizes much of this blue...
by PeteM
Mon Apr 05, 2021 5:55 am
Forum: Beginner's corner
Topic: Using a pair of cameras with a stereo microscope
Replies: 32
Views: 1300

Re: Using a pair of cameras with a stereo microscope

The original Nikon SMZ-10 stereo microscope had a trinocular port that could be switched to the left or right eyetube. Far more convenient that dueling cameras attached to each eyetube and available (used) for under $1K. That said, stereo microscopes don't generally take the best low magnification p...
by PeteM
Sun Apr 04, 2021 1:09 am
Forum: Illumination Techniques
Topic: Lighting the stage on a compound microscope
Replies: 9
Views: 349

Re: Lighting the stage on a compound microscope

As Crb5 suggests, you should be able to get a 1 to 3 watt gooseneck lamp for maybe $20 from Ikea, Amazon, Ebay, etc.. I also use higher power LED flashlights (around 800 lumens) and hold them in anything from articulated stands to table mount microphone stands. This isn't a recommendation (just one ...
by PeteM
Sat Apr 03, 2021 11:59 pm
Forum: Illumination Techniques
Topic: Lighting the stage on a compound microscope
Replies: 9
Views: 349

Re: Lighting the stage on a compound microscope

You can probably get a small LED lamp (on a gooseneck, articulated holder, etc.) to come in from the side and have enough clearance with a 4x objective (40x total magnification). Polarizers might help a bit to see the scratch detail (one over the LED, one above the objective). Closer to an ideal too...
by PeteM
Sat Apr 03, 2021 4:46 pm
Forum: Pictures and Videos
Topic: Acetaminophen
Replies: 6
Views: 309

Re: Acetaminophen

Great images, Javier. It sounds like you bought some better polarizers?
by PeteM
Sat Apr 03, 2021 2:14 am
Forum: Microscopes and optics
Topic: Fix the slide holder
Replies: 4
Views: 266

Re: Fix the slide holder

Microb - It likely got bent at some point. Use a straightedge, find the inflection point, and bend it back. Ideally, it just barely floats above the stage.
by PeteM
Fri Apr 02, 2021 1:16 am
Forum: Microscopes and optics
Topic: Disassembling a Leica HC Plan Fluotar 20X/0.5 NA lens and fixing crud advice needed
Replies: 7
Views: 360

Re: Disassembling a Leica HC Plan Fluotar 20X/0.5 NA lens and fixing crud advice needed

It looks like a goner to me as well. But in the interest of exploration, a couple thoughts. First is that to get into most of the Leica infinity objectives, you need to remove the shroud covering the nose of the objective. This sometimes screws on. Sometimes affixed with cement. A Leica repair tech ...
by PeteM
Fri Apr 02, 2021 1:06 am
Forum: Camera systems and imaging
Topic: Looking for stereoscopes with cameras
Replies: 3
Views: 286

Re: Looking for stereoscopes with cameras

Greg, I'd be inclined to separate the decision about what stereo microscope to buy from what Bluetooth-enabled camera to buy. A good stereo scope might last a decade. Technology (or student use) might make your cameras obsolete in three years. If your students are old enough to take care you might w...