Search found 1632 matches

by PeteM
Sat May 08, 2021 10:52 pm
Forum: Pictures and Videos
Topic: melosira + pleurosigma
Replies: 11
Views: 206

Re: melosira + pleurosigma

Count me in as thoroughly admiring that shot.

To me, capturing two side by side like that is 4x the challenge and 4x the excellence when done so well -- compared to equally excellent individual shots.
by PeteM
Sat May 08, 2021 5:26 pm
Forum: Microscopes and optics
Topic: AO Stereo Star Zoom 580 Help Needed
Replies: 13
Views: 311

Re: AO Stereo Star Zoom 580 Help Needed

Steven - worst case -- I suspect you might find one of the entire #570 or #580 heads reasonably priced. Seems that heads show up far more often than the stands.
by PeteM
Thu May 06, 2021 3:14 am
Forum: Introduce yourself
Topic: Newbie interested in mycology
Replies: 4
Views: 176

Re: Newbie interested in mycology

Happy to send you a copy of a guide to various microscope brands and models - once you have enough posts to send a message. Could help you read up on something you might find locally. $500 would be a terrific deal on something like an Olympus BHS with SPlan objectives, a good deal on a BHTU with DPl...
by PeteM
Mon May 03, 2021 8:40 pm
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 265

Re: eyepiece camera vs phone

Might add, that another option is a 1" sensor C-mount camera. These range from $100 for a used mirrorless camera body with a c-mount adapter to $500 for something like a 20mp Rising Cam with good software. C-mount adapters (.6x,.7x etc. for image sizing) are cheap and these 10-20mp mirrorless camera...
by PeteM
Mon May 03, 2021 8:22 pm
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 265

Re: eyepiece camera vs phone

+1 to the above. If you have a spare phone camera and mount the proper eyepiece to it, it is about as convenient to swap in and out as a USB camera and will generally provide better images than all but the more expensive USB variants. A holder including a remote phone camera release suitable for man...
by PeteM
Mon May 03, 2021 8:12 pm
Forum: Specimens, samples and slides
Topic: Round slides?
Replies: 7
Views: 190

Re: Round slides?

If all else fails, you might try cheap UV filters for cameras? Around $4 could get you one with pretty flat glass and coatings in sizes (once pulled out of the threaded ring) around 2mm smaller than the stated thread size. https://www.ebay.com/itm/254002815427?hash=item3b23bf61c3:g:Ch8AAOSwG25b~rFH
by PeteM
Sat May 01, 2021 8:48 pm
Forum: Pictures and Videos
Topic: water lens under UV...
Replies: 6
Views: 186

Re: water lens under UV...

Hobbyst46 wrote:
Sat May 01, 2021 6:19 pm
. . .Tiny floating water plants, a circular leaf with tiny root, named "water lens". . .
Thanks.
by PeteM
Sat May 01, 2021 6:02 pm
Forum: Pictures and Videos
Topic: water lens under UV...
Replies: 6
Views: 186

Re: water lens under UV...

Curious what a "water lens" means, as photographed here? Is it just a drop of water with pond scum underneath and further magnified 400x?
by PeteM
Fri Apr 30, 2021 6:06 am
Forum: Microscopes and optics
Topic: Anyone know about this weird Leitz condenser?
Replies: 1
Views: 142

Re: Anyone know about this weird Leitz condenser?

That condenser is new to me. However, the idea of using an objective as a condensing lens has been around for a while - it's one way to make sure a specimen is illuminated with condenser whose numerical aperture matches the objective - and thus achieves the full resolution it might be capable of pro...
by PeteM
Thu Apr 29, 2021 1:17 am
Forum: Microscopes and optics
Topic: Why are numerical aperture and color correction related?
Replies: 8
Views: 279

Re: Why are numerical aperture and color correction related?

You might look up "Airy disc." Here's ar rough explanation. Diffraction limits the ability to distinguish / resolve any two closely-spaced points on a specimen when magnified in a microscope. Two very closely spaced points that we're trying to see as distinct, can be represented as Airy discs that t...
by PeteM
Wed Apr 28, 2021 4:27 am
Forum: Sandbox
Topic: questions about an AO Fifty Phase
Replies: 6
Views: 512

Re: questions about an AO Fifty Phase

It's an homage to Frits Zernike, who won a Nobel Prize for what most makers now call "phase contrast." It's also used, by several researchers I know, to describe the rotary type phase condenser with multiple phase annuli in it -- perhaps because that's what most post-WWII makers ended up providing w...
by PeteM
Tue Apr 27, 2021 3:47 pm
Forum: Sandbox
Topic: questions about an AO Fifty Phase
Replies: 6
Views: 512

Re: questions about an AO Fifty Phase

The color codes on the rotating ("Zernicke type") phase condenser correspond to the phase contrast annuli inside - and as noted above should match up with the phase contrast objectives color banding you have. This is one of the most affordable phase contrast scopes available, but with an old illumin...
by PeteM
Mon Apr 26, 2021 2:18 pm
Forum: Microscopes and optics
Topic: What does Nikon Fluor indicate?
Replies: 4
Views: 154

Re: What does Nikon Fluor indicate?

Might add that since our eyes are drawn to where we focus (say, the center) and can accommodate a bit if we shift our gaze even a regular achromat looks pretty good for biological specimens. Naturally, the fluor even better. Someone with more focus-stacking experience than I might be able to comment...
by PeteM
Mon Apr 26, 2021 2:11 pm
Forum: Microscopes and optics
Topic: What does Nikon Fluor indicate?
Replies: 4
Views: 154

Re: What does Nikon Fluor indicate?

You'll see Nikon objectives in both "fluor" and "plan fluor" versions. As you surmise, the type you cited was intended more for fluorescence. Nice high numerical aperture to gather faint fluorescence. Glass that doesn't absorb so much UV. The phase contrast useful as a companion to fluorescence for ...
by PeteM
Mon Apr 26, 2021 5:44 am
Forum: Home-made microscope adaptations
Topic: Making a negative meniscus lense ?
Replies: 15
Views: 542

Re: Making a negative meniscus lense ?

You could try removing the condenser, dropping it way down with the top lens off or a wider angle top lens substituted, or replacing it entirely as needed with a wide angle condenser modified from another brand. Maybe add a diffuser to the field lens if the field is still uneven while trying the abo...
by PeteM
Sun Apr 25, 2021 3:53 am
Forum: Illumination Techniques
Topic: Epi DIC woes
Replies: 67
Views: 13770

Re: Epi DIC woes

The images look great. Persistence rewarded!
by PeteM
Sat Apr 24, 2021 11:02 pm
Forum: For forum members who want to buy and sell equipment
Topic: Need 28mm eyepieces
Replies: 16
Views: 332

Re: Need 28mm eyepieces

Assuming the 28mm is some odd internal diameter -- the simplest thing might be to buy 15x or 20x eyepieces that fit the standard 23mm tube and hold them in a bushing. Aluminum adapters (black anodized) are readily available to go from 30 or 30.5mm to the 23mm of standard eyepieces. These could be tu...
by PeteM
Sat Apr 24, 2021 4:59 pm
Forum: Microscopes and optics
Topic: Nikon Optiphot vs Optiphot 2
Replies: 4
Views: 250

Re: Nikon Optiphot vs Optiphot 2

Most Nikon replaceable-type nosepieces should fit either of the Optiphots. It's just the one reversed nosepiece with a correction lens (or one destined for an inverted scope) that you may come across used and find not suitable for the Optiphot 2. Optiphot 2 is a great choice - but often harder to fi...
by PeteM
Sat Apr 24, 2021 6:45 am
Forum: Miscellaneous
Topic: Cleaning the Immersion Objective
Replies: 14
Views: 464

Re: Cleaning the Immersion Objective

To add, if you do get some oil on a dry objective - just clean it off ASAP. Same approach. Blot up most of the oil. Then carefully use a bit of lens cleaner on a tissue to remove what little is left. Use multiples and avoid soaking the front element with any solvent or cleaner. Good quality objectiv...
by PeteM
Sat Apr 24, 2021 6:19 am
Forum: Miscellaneous
Topic: Cleaning the Immersion Objective
Replies: 14
Views: 464

Re: Cleaning the Immersion Objective

This is one of those questions where different users with long experience may give somewhat different advice. The good thing about that is that it more or less demonstrates that people have managed different cleaning tissues (lens tissue, delicate task Kimwipes, foam swabs, clean cotton, clean micro...
by PeteM
Thu Apr 22, 2021 6:18 pm
Forum: Microscopes and optics
Topic: Nikon Optiphot vs Optiphot 2
Replies: 4
Views: 250

Re: Nikon Optiphot vs Optiphot 2

Both Nikon Optiphot models are excellent. As Hobbyist says, both have interchangeable nosepieces. As with Olympus BHT and BHS, they are super handy for someone who uses a variety of imaging methods. You could have one "dry" for brightfield, maybe one for low magnifications (Nikon made very good fini...
by PeteM
Thu Apr 22, 2021 5:41 am
Forum: Microscopes and optics
Topic: Most useful magnifications for phase contrast
Replies: 13
Views: 469

Re: Most useful magnifications for phase contrast

It sounds like the scope you're considering takes interchangeable nosepieces. If so, you could have one turret for dry objectives and one for oil immersion. For example, with something like a 4x or 10x as a finder (with so much clearance it wouldn't come near oil on a slide) and a 50x-60x and/or 100...
by PeteM
Tue Apr 20, 2021 7:21 pm
Forum: Beginner's corner
Topic: Knurled ring on 60X objective
Replies: 6
Views: 220

Re: Knurled ring on 60X objective

If it turns, there are two likely possibilities. One is an iris inside to adjust contrast. Useful for darkfield. If you look through the lens you'll see an aperture opening and closing. The other is to adjust lens elements to adjust for cover slip thickness. If so, you'll likely see graduations alon...
by PeteM
Tue Apr 20, 2021 3:57 pm
Forum: Microscopes and optics
Topic: Most useful magnifications for phase contrast
Replies: 13
Views: 469

Re: Most useful magnifications for phase contrast

AO/Reichert made a phase condenser with a single insert that could be slid in or out for phase contrast. They apparently thought a 40x was most useful, since this was the default configuration. For many really interested in cell details, it's probably a good decision. Working with kids, I find a 20x...
by PeteM
Tue Apr 20, 2021 1:13 am
Forum: Beginner's corner
Topic: Help to make a purchasing decision: Swift SW350T vs OMAX M83ES vs AmScope T120
Replies: 6
Views: 220

Re: Help to make a purchasing decision: Swift SW350T vs OMAX M83ES vs AmScope T120

To add on the AmScope T120 - it lists the lighting as LED 6 volts 20 watts. Could be the power supply draws or could supply 20 watts, but I seriously doubt the light emitting diode runs at 20 watts.
by PeteM
Tue Apr 20, 2021 1:04 am
Forum: Beginner's corner
Topic: Help to make a purchasing decision: Swift SW350T vs OMAX M83ES vs AmScope T120
Replies: 6
Views: 220

Re: Help to make a purchasing decision: Swift SW350T vs OMAX M83ES vs AmScope T120

I'd consider either 20x or 25x microscope eyepieces to be near-worthless additions for marketing purposes -- and wouldn't consider either a decision factor. Plan achromat versus regular achromat objectives would be a worthwhile addition for those interested in taking photos or making movies. They'll...
by PeteM
Mon Apr 19, 2021 2:49 am
Forum: Miscellaneous
Topic: microspectrophotometry
Replies: 1
Views: 189

Re: microspectrophotometry

You might find a surplus scope from the semiconductor industry, used to find "hot spots" on chips and boards as a stand or chassis on the scope end of things. These typically used reflected objectives (sort of like telescope mirrors) to avoid reducing the IR signal too much. And then a camera sensor...
by PeteM
Sun Apr 18, 2021 8:00 pm
Forum: Beginner's corner
Topic: should i buy Bresser phase contrast series 57?
Replies: 8
Views: 448

Re: should i buy Bresser phase contrast series 57?

Bresser, like AmScope and many others, puts their brand on generic Chinese scopes. Find a picture of the Bresser 57 model you're considering and you might find reviews of essentially the same microscope, out of the same factory under another brand name. I have mixed feelings about the $1000 or so Ch...