Search found 1645 matches

by PeteM
Sun May 16, 2021 4:01 am
Forum: Introduce yourself
Topic: Hello fellow microscopists!
Replies: 3
Views: 73

Re: Hello fellow microscopists!

His interest is fungi.
by PeteM
Sun May 16, 2021 2:27 am
Forum: Introduce yourself
Topic: Hello fellow microscopists!
Replies: 3
Views: 73

Re: Hello fellow microscopists!

Welcome, Ben.

If they do a remake of the Dustin Hoffman movie, The Graduate, pretty sure the one-word vocational advice given will be more like "microbiology" than "plastics." Where are you going to school? A friend used to head up microbiology at UCSC and still does some research there.
by PeteM
Sat May 15, 2021 11:40 pm
Forum: Microscopes and optics
Topic: Achro vs. Plan Achro vs. Plan Apo stereo CMO objectives?
Replies: 4
Views: 101

Re: Achro vs. Plan Achro vs. Plan Apo stereo CMO objectives?

Thanks for that (as usual) informative reply. Much appreciated.
by PeteM
Sat May 15, 2021 9:21 pm
Forum: Microscopes and optics
Topic: Achro vs. Plan Achro vs. Plan Apo stereo CMO objectives?
Replies: 4
Views: 101

Achro vs. Plan Achro vs. Plan Apo stereo CMO objectives?

I'm hoping that someone with more experience can give an indication of what one gains with better corrected CMO stereo objectives? Most of my somewhat better CMO scopes (e.g. Wild M3C, Wild M8, Nikon SMZ-10) have achro objectives and achro supplemental lenses. They're generally fine for viewing, wit...
by PeteM
Sat May 15, 2021 5:09 pm
Forum: Important announcements
Topic: New Microscopy Wiki!
Replies: 3
Views: 107

Re: New Microscopy Wiki!

Sounds like a great addition. Thanks, Oliver.
by PeteM
Thu May 13, 2021 5:20 pm
Forum: My microscope
Topic: My Amscope T660
Replies: 1
Views: 88

Re: My Amscope T660

. . .- apparently the substage condenser holder is not the one that was supposed to be on a T660 . . . Maintaining and managing bills of materials often gets lost when dealers (AmScope, Omax, Swift, etc.) buy batches of scopes and parts. As parts get revised or suppliers changed inconsistencies (an...
by PeteM
Wed May 12, 2021 8:11 pm
Forum: Microscopes and optics
Topic: Leica DM questions
Replies: 7
Views: 210

Re: Leica DM questions

A few answers: - Yes, that's the listing of Leica objectives. There's another one out there that goes back to Delta optics days as well. - Yes, what I've kept is a DMLB. The only thing I don't like about it is the two-speed fine focus "transmission" isn't quite perfect in either example I've had. Th...
by PeteM
Wed May 12, 2021 4:18 pm
Forum: Microscopes and optics
Topic: Leica DM questions
Replies: 7
Views: 210

Re: Leica DM questions

Leica's 63x Plan Apo has a 1.3na and a larger working distance - so you might want to consider that as an option. Essentially the same resolution as a high-end 100x, but able to see a few more microns on a cover slip.
by PeteM
Wed May 12, 2021 1:27 pm
Forum: Microscopes and optics
Topic: How much worse is the phase version of an objective for brightfileld
Replies: 11
Views: 224

Re: How much worse is the phase version of an objective for brightfileld

I'd be inclined to bide your time and look for a Nikon Optiphot with the CFN objectives. The regular CF plan achros are also pretty good and often cheap if you want some magnification right now. Even the CFN Plan Apos are sometimes affordable. Starting from scratch either the Nikon or Olympus are fa...
by PeteM
Wed May 12, 2021 1:09 pm
Forum: Microscopes and optics
Topic: Leica DM questions
Replies: 7
Views: 210

Re: Leica DM questions

That will be a wonderful scope - congratulations. There is a listing of Leica HC objectives online that lists the prism combinations that work with each. Sorry, I don't have a link - downloaded it a year or two ago. It should yield to a search. I can also send you the PDF if you PM me your contact i...
by PeteM
Wed May 12, 2021 2:44 am
Forum: Microscopes and optics
Topic: Leica DM series K1a versus K1b DIC prisms?
Replies: 0
Views: 59

Leica DM series K1a versus K1b DIC prisms?

Hoping someone knows the difference between the K1a and K1b condenser-mounted DIC prisms? These are meant to provide DIC with a 5x objective and far as I can tell require the top condenser lens down for the K1a and maybe allow it to be up for the K1b??? If you've used Leica DM series DIC with a 5x P...
by PeteM
Tue May 11, 2021 9:52 pm
Forum: Microscopes and optics
Topic: How much worse is the phase version of an objective for brightfileld
Replies: 11
Views: 224

Re: How much worse is the phase version of an objective for brightfileld

You'd clearly see the difference in an A-B comparison. However, it might not bother you without that direct comparison. Depending upon what you want to view, if protists and other live and near-transparent cells are foremost among your subjects -- I'm inclined to agree with Bram. 10x is a pretty dec...
by PeteM
Sun May 09, 2021 11:42 pm
Forum: Illumination Techniques
Topic: Ptychographic Microscopy
Replies: 4
Views: 230

Re: Ptychographic Microscopy

You've got us intrigued, Greg. What size mirrors do you need? Could something be scavenged from a reflected IR objective, a Xenon lamp, or ???
by PeteM
Sat May 08, 2021 10:52 pm
Forum: Pictures and Videos
Topic: melosira + pleurosigma
Replies: 11
Views: 298

Re: melosira + pleurosigma

Count me in as thoroughly admiring that shot.

To me, capturing two side by side like that is 4x the challenge and 4x the excellence when done so well -- compared to equally excellent individual shots.
by PeteM
Sat May 08, 2021 5:26 pm
Forum: Microscopes and optics
Topic: AO Stereo Star Zoom 580 Help Needed
Replies: 13
Views: 414

Re: AO Stereo Star Zoom 580 Help Needed

Steven - worst case -- I suspect you might find one of the entire #570 or #580 heads reasonably priced. Seems that heads show up far more often than the stands.
by PeteM
Thu May 06, 2021 3:14 am
Forum: Introduce yourself
Topic: Newbie interested in mycology
Replies: 4
Views: 252

Re: Newbie interested in mycology

Happy to send you a copy of a guide to various microscope brands and models - once you have enough posts to send a message. Could help you read up on something you might find locally. $500 would be a terrific deal on something like an Olympus BHS with SPlan objectives, a good deal on a BHTU with DPl...
by PeteM
Mon May 03, 2021 8:40 pm
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 345

Re: eyepiece camera vs phone

Might add, that another option is a 1" sensor C-mount camera. These range from $100 for a used mirrorless camera body with a c-mount adapter to $500 for something like a 20mp Rising Cam with good software. C-mount adapters (.6x,.7x etc. for image sizing) are cheap and these 10-20mp mirrorless camera...
by PeteM
Mon May 03, 2021 8:22 pm
Forum: Beginner's corner
Topic: eyepiece camera vs phone
Replies: 7
Views: 345

Re: eyepiece camera vs phone

+1 to the above. If you have a spare phone camera and mount the proper eyepiece to it, it is about as convenient to swap in and out as a USB camera and will generally provide better images than all but the more expensive USB variants. A holder including a remote phone camera release suitable for man...
by PeteM
Mon May 03, 2021 8:12 pm
Forum: Specimens, samples and slides
Topic: Round slides?
Replies: 7
Views: 236

Re: Round slides?

If all else fails, you might try cheap UV filters for cameras? Around $4 could get you one with pretty flat glass and coatings in sizes (once pulled out of the threaded ring) around 2mm smaller than the stated thread size. https://www.ebay.com/itm/254002815427?hash=item3b23bf61c3:g:Ch8AAOSwG25b~rFH
by PeteM
Sat May 01, 2021 8:48 pm
Forum: Pictures and Videos
Topic: water lens under UV...
Replies: 6
Views: 239

Re: water lens under UV...

Hobbyst46 wrote:
Sat May 01, 2021 6:19 pm
. . .Tiny floating water plants, a circular leaf with tiny root, named "water lens". . .
Thanks.
by PeteM
Sat May 01, 2021 6:02 pm
Forum: Pictures and Videos
Topic: water lens under UV...
Replies: 6
Views: 239

Re: water lens under UV...

Curious what a "water lens" means, as photographed here? Is it just a drop of water with pond scum underneath and further magnified 400x?
by PeteM
Fri Apr 30, 2021 6:06 am
Forum: Microscopes and optics
Topic: Anyone know about this weird Leitz condenser?
Replies: 1
Views: 174

Re: Anyone know about this weird Leitz condenser?

That condenser is new to me. However, the idea of using an objective as a condensing lens has been around for a while - it's one way to make sure a specimen is illuminated with condenser whose numerical aperture matches the objective - and thus achieves the full resolution it might be capable of pro...
by PeteM
Thu Apr 29, 2021 1:17 am
Forum: Microscopes and optics
Topic: Why are numerical aperture and color correction related?
Replies: 8
Views: 364

Re: Why are numerical aperture and color correction related?

You might look up "Airy disc." Here's ar rough explanation. Diffraction limits the ability to distinguish / resolve any two closely-spaced points on a specimen when magnified in a microscope. Two very closely spaced points that we're trying to see as distinct, can be represented as Airy discs that t...
by PeteM
Wed Apr 28, 2021 4:27 am
Forum: Sandbox
Topic: questions about an AO Fifty Phase
Replies: 6
Views: 624

Re: questions about an AO Fifty Phase

It's an homage to Frits Zernike, who won a Nobel Prize for what most makers now call "phase contrast." It's also used, by several researchers I know, to describe the rotary type phase condenser with multiple phase annuli in it -- perhaps because that's what most post-WWII makers ended up providing w...
by PeteM
Tue Apr 27, 2021 3:47 pm
Forum: Sandbox
Topic: questions about an AO Fifty Phase
Replies: 6
Views: 624

Re: questions about an AO Fifty Phase

The color codes on the rotating ("Zernicke type") phase condenser correspond to the phase contrast annuli inside - and as noted above should match up with the phase contrast objectives color banding you have. This is one of the most affordable phase contrast scopes available, but with an old illumin...
by PeteM
Mon Apr 26, 2021 2:18 pm
Forum: Microscopes and optics
Topic: What does Nikon Fluor indicate?
Replies: 4
Views: 193

Re: What does Nikon Fluor indicate?

Might add that since our eyes are drawn to where we focus (say, the center) and can accommodate a bit if we shift our gaze even a regular achromat looks pretty good for biological specimens. Naturally, the fluor even better. Someone with more focus-stacking experience than I might be able to comment...
by PeteM
Mon Apr 26, 2021 2:11 pm
Forum: Microscopes and optics
Topic: What does Nikon Fluor indicate?
Replies: 4
Views: 193

Re: What does Nikon Fluor indicate?

You'll see Nikon objectives in both "fluor" and "plan fluor" versions. As you surmise, the type you cited was intended more for fluorescence. Nice high numerical aperture to gather faint fluorescence. Glass that doesn't absorb so much UV. The phase contrast useful as a companion to fluorescence for ...
by PeteM
Mon Apr 26, 2021 5:44 am
Forum: Home-made microscope adaptations
Topic: Making a negative meniscus lense ?
Replies: 17
Views: 757

Re: Making a negative meniscus lense ?

You could try removing the condenser, dropping it way down with the top lens off or a wider angle top lens substituted, or replacing it entirely as needed with a wide angle condenser modified from another brand. Maybe add a diffuser to the field lens if the field is still uneven while trying the abo...