Search found 1724 matches

by PeteM
Sun Jul 25, 2021 4:45 pm
Forum: Specimens, samples and slides
Topic: What magnifications do pathologists typically work with?
Replies: 4
Views: 90

Re: What magnifications do pathologists typically work with?

Thanks, Tom. I was hoping you might see this and reply.

What puzzled me is that the surgeon told my friend that he scanned the bit he carved out "at 10x magnification." I thought that surely must have been at least 100x, unless staining alone was a clear indicator.
by PeteM
Sun Jul 25, 2021 5:05 am
Forum: Specimens, samples and slides
Topic: What magnifications do pathologists typically work with?
Replies: 4
Views: 90

Re: What magnifications do pathologists typically work with?

Would you know what magnification is typically used to distinguish normal from cancerous cells? I was surprised to hear 10x (1x objective, 10 eyepieces?) and would imagine a 1x or 2x would be more for scanning the slide than cell type ID.
by PeteM
Sun Jul 25, 2021 5:03 am
Forum: Pictures and Videos
Topic: Made another compilation video
Replies: 6
Views: 113

Re: Made another compilation video

Some great "catches" . . . thanks for posting.
by PeteM
Sun Jul 25, 2021 1:50 am
Forum: Specimens, samples and slides
Topic: What magnifications do pathologists typically work with?
Replies: 4
Views: 90

What magnifications do pathologists typically work with?

A friend's father recently had a skin cancer removed and said the surgeon briefly stopped to check the margins by viewing the sample (per the surgeon) at 10x and decided to take a bit more. This was apparently an old school compound microscope - nothing fancy. Would this be 100x (a 10x objective) ra...
by PeteM
Sat Jul 24, 2021 4:21 pm
Forum: Microscopes and optics
Topic: Leitz pin connector?
Replies: 9
Views: 246

Re: Leitz pin connector?

I've converted two or three power supplies to "banana jacks." These can be had already paired for both the male and female sides (a single plug) and decent ones will handle up to 30 volts and 10-15 amps. Just need to drill two holes in the chassis to fit them and they're easy to plug in and out.
by PeteM
Thu Jul 22, 2021 6:16 am
Forum: Pictures and Videos
Topic: Diatoms in epi DIC
Replies: 18
Views: 390

Re: Diatoms in epi DIC

Beautiful images. Thanks for posting them.
by PeteM
Sun Jul 18, 2021 2:58 am
Forum: Microscopes and optics
Topic: Leitz Wetzlar microscope base and light source
Replies: 8
Views: 205

Re: Leitz Wetzlar microscope base and light source

Looks like a micro projector, with prism at the top, where an eyepiece would normally be located?
by PeteM
Fri Jul 16, 2021 4:09 pm
Forum: Illumination Techniques
Topic: Converting Brightfield Microscope To DIC
Replies: 30
Views: 1028

Re: Converting Brightfield Microscope To DIC

No problem. I just didn't want others to be confused. Closing the condenser iris down does increase contrast and depth of field, but at the expensive of resolution.
by PeteM
Fri Jul 16, 2021 3:02 am
Forum: Illumination Techniques
Topic: Converting Brightfield Microscope To DIC
Replies: 30
Views: 1028

Re: Converting Brightfield Microscope To DIC

For comparison, some actual DIC video images. Go in about 10 seconds to get an idea of the detail and contrast possible with DIC:

https://www.youtube.com/watch?v=UFErSrAr5xM
by PeteM
Wed Jul 14, 2021 4:48 am
Forum: Microscopes and optics
Topic: How to unstick correction collar on objective?
Replies: 8
Views: 261

Re: How to unstick correction collar on objective?

One thing that has worked for me (sometimes) is to apply a little bit of heat using a hot air pencil (sort of like a tiny blow dryer) and gently work the iris back and forth a bit. This will often soften any hardened lube enough to free the iris -- and allow a drop of oil subsequently applied to pen...
by PeteM
Mon Jul 12, 2021 6:23 am
Forum: Microscopy accessories
Topic: Can one adjust a condenser dovetail?
Replies: 17
Views: 300

Re: Can one adjust a condenser dovetail?

If you have a 3D printer and enough room under the stage (which is likely), you could 3D print an adapter/carrier for the Nikon 1.4na condenser. Top part is female with some setscrews coming in from the side to capture the Nikon dovetail. Bottom replicates your scope's smaller dovetail. Pretty simpl...
by PeteM
Mon Jul 12, 2021 2:01 am
Forum: Microscopy accessories
Topic: Can one adjust a condenser dovetail?
Replies: 17
Views: 300

Re: Can one adjust a condenser dovetail?

Assuming the dovetail is just a bit too large, it can be turned down in a lathe. It can be chucked either internally or externally, and a narrow profile turning tool used to replicate the dovetail angle (just a bit smaller). Kind of a shame to modify such a nice Nikon condenser through. FWIW an unmo...
by PeteM
Sun Jul 11, 2021 4:42 am
Forum: Microscopes and optics
Topic: Strange flare in Nikon CF N plan apo 20/0.75 objective
Replies: 26
Views: 591

Re: Strange flare in Nikon CF N plan apo 20/0.75 objective

One possibility is flare, as you suggest, introduced by the thicker section. Apos have more lens elements and thus are more susceptible to internal reflections. You might try carefully stopping down the field and condenser irises for better control - but that thick section may be introducing it's ow...
by PeteM
Sun Jul 11, 2021 3:40 am
Forum: Beginner's corner
Topic: Should the condenser diaphragm work when using DIC?
Replies: 9
Views: 255

Re: Should the condenser diaphragm work when using DIC?

You're right -- the condenser iris should work.

With phase contrast, many makers disable the iris on the positions where phase rings are located to avoid closing the iris down over them.

Sounds like the maker didn't want to go to the trouble of making a dedicated DIC condenser?
by PeteM
Mon Jul 05, 2021 3:14 am
Forum: Home-made microscope adaptations
Topic: Stage adaption
Replies: 5
Views: 236

Re: Stage adaption

Good point; the B&L "R" stand is excellent.
by PeteM
Mon Jul 05, 2021 12:44 am
Forum: Home-made microscope adaptations
Topic: Stage adaption
Replies: 5
Views: 236

Re: Stage adaption

Depending upon what stereo scope you have, just buying a generic (Chinese) stands with a coarse and FINE focus is another option. They run about $150 for ones that are modeled (copied) on Nikon units. The ones I've used have a 76mm ring; but could be adapted to other formats. Others have chopped off...
by PeteM
Thu Jul 01, 2021 6:41 pm
Forum: Camera systems and imaging
Topic: BH2 to DSLR distance confusion
Replies: 15
Views: 329

Re: BH2 to DSLR distance confusion

Assuming you're using an OEM setup, you might double check diopter adjustments on your eyepieces to see if they are set to the zero position to begin. Get your DSLR in focus on the trinocular tube, then adjust the eyepieces to be in focus for your eyes. If making a trinocular camera adapter, I'll ey...
by PeteM
Tue Jun 29, 2021 1:31 am
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 41
Views: 1093

Re: Questions about metallurgy on Olympus

FWIW, the BHS has an adjustable stage that could be lowered somewhat for Epi use.

I'd still keep an eye out for a complete epi scope, rather than solely hoping to acquire the bits to outfit a BHS for reflected use one by one.
by PeteM
Mon Jun 28, 2021 4:06 am
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 41
Views: 1093

Re: Questions about metallurgy on Olympus

1) As noted above, you can buy the parts for your BHS. Just not likely the most cost-effective route.

2) Yes.

3) See notes above. Best to get original documentation, perhaps from Alan Woods' site.

4) Correct. In this case my recollection is that it sort of barely not-quite-right works.
by PeteM
Sun Jun 27, 2021 9:30 pm
Forum: My microscope
Topic: Nikon Optiphot with DIC
Replies: 21
Views: 953

Re: Nikon Optiphot with DIC

The prisms hidden away in the nosepiece also explain why you weren't able to get exinction in simple polarization. Move the nosepiece to an open position (no prism) and all should be well.
by PeteM
Sun Jun 27, 2021 7:12 am
Forum: Microscopes and optics
Topic: Questions about metallurgy on Olympus
Replies: 41
Views: 1093

Re: Questions about metallurgy on Olympus

My experience is that finding the bits to add epi DIC to a system can cost more and take more time than buying a properly configured system complete. If you want to use your BHS as the stand, then something like a BH2-UMA illuminator ($250? complete with lamp house or another $150 for that???) with ...
by PeteM
Sat Jun 26, 2021 6:48 pm
Forum: Microscopes and optics
Topic: Had To Buy IT!!
Replies: 12
Views: 552

Re: Had To Buy IT!!

John's scope (our topic post) is looking ever better. All those wonderful SZH optics and mechanisms, PLUS what is likely today a 1200 euro objective changer.
by PeteM
Sat Jun 26, 2021 2:37 pm
Forum: Microscopes and optics
Topic: Had To Buy IT!!
Replies: 12
Views: 552

Re: Had To Buy IT!!

Thanks, Rorshach.

Not seeing any prices on the Opto site, I'm reminded of the old J.P. Morgan quote "if you have to ask how much it costs, you can't afford it . . ."
by PeteM
Sat Jun 26, 2021 7:12 am
Forum: Microscopes and optics
Topic: Had To Buy IT!!
Replies: 12
Views: 552

Re: Had To Buy IT!!

Rorschach wrote:
Sat Jun 26, 2021 6:09 am
. . . Fortunately a third party objective slider (of apparently high quality) can be found for the Wilds/Leicas as well. So expansion route is secured should I feel the need :D
Haven't seen that. Would you happen to have a link?
by PeteM
Thu Jun 24, 2021 10:45 pm
Forum: Illumination Techniques
Topic: Epi DIC woes
Replies: 72
Views: 15578

Re: Epi DIC woes

There's sort of a trade off in the choice of shear angles for DIC; between contrast and resolution. On the transmitted side, Olylmpus offered three choices for each magnification to better match the subject. Seems the reflected side, as Stephen says, was optimized for wafer inspection, tiny surface ...
by PeteM
Thu Jun 24, 2021 5:07 am
Forum: Illumination Techniques
Topic: Microscope illuminator flash adjustable LED light housing
Replies: 9
Views: 519

Re: Microscope illuminator flash adjustable LED light housing

Those flash adapters as well as other scope add-ons (darkfield filters etc.) are designed and 3D printed by Saul, who occasionally posts on this forum.
by PeteM
Wed Jun 23, 2021 6:10 am
Forum: Microscopes and optics
Topic: Nikon SMZ-10 trinocular head on a Nikon SMZ-U?
Replies: 3
Views: 108

Re: Nikon SMZ-10 trinocular head on a Nikon SMZ-U?

Thanks, Stephen. FWIW, I don't have the Nikon 10x/24 eyepieces for the SMZ-U, but a set of Mitutoyo 10x/24 seem to hold up OK to the edges of the SMZ-10 binocular tubes and ever so slightly wider than the Nikon 10x/23 eyepieces. Haven't taken any photos yet - there might be some artifacts not visual...
by PeteM
Tue Jun 22, 2021 11:04 pm
Forum: Microscopes and optics
Topic: Nikon SMZ-10 trinocular head on a Nikon SMZ-U?
Replies: 3
Views: 108

Nikon SMZ-10 trinocular head on a Nikon SMZ-U?

Curious if there is any reason to not use a Nikon SMZ-10 trinocular head on a Nikon SMZ-U body? I've recently acquired and fixed up a Nikon SMZ-U. It's now working fine but came minus a photo port. In doing a quick check, an SMZ-10 head seems to work just fine on the SMZ-U using the 2.5x photo relay...
by PeteM
Tue Jun 22, 2021 9:19 pm
Forum: My microscope
Topic: Nikon Optiphot with DIC
Replies: 21
Views: 953

Re: Nikon Optiphot with DIC

Hoping you can tell us what DIC prisms are being used to get DIC at 20x? Does that slider have a DIC prism in it? And one or more DIC prisms in the condenser?

It does look to be a fine scope, even with not-quite-right DIC for the time being.