Search found 2727 matches

by MicroBob
Fri Jun 11, 2021 8:21 pm
Forum: Microscopes and optics
Topic: Wattage on light source
Replies: 5
Views: 141

Re: Wattage on light source

Hi Marl, many important microscope properties can' t be read from the technical data. You can upgrade the objectives or LED but you can't upgrade e.g. the guides that hold the table. And for achromatic, semi-plan and plan there is no common standard. The Leitz NPL Fluotars are planar over a field of...
by MicroBob
Fri Jun 11, 2021 12:56 pm
Forum: Beginner's corner
Topic: which is the real DIN 160mm objective?
Replies: 11
Views: 273

Re: which is the real DIN 160mm objective?

When looking for used objectives of higher power one can copy the image into irfan view and mesure length and thread diameter to determin the object distance / parfocal distance.
In Klaus Henkel's "Mikrofibel" there is a table with specification values for different manufacturers.

Bob
by MicroBob
Fri Jun 11, 2021 5:31 am
Forum: Introduce yourself
Topic: Intro
Replies: 6
Views: 178

Re: Intro

Hi Griff, welcome to the forum! I'm sure that you will find here a lot of inspiration to keep your microscopy hobby going. For someone who has other interests or duties too microscopy is a quite practical hobby as it can be suspended for a time and the stuff placed in a cupboard, not possible with a...
by MicroBob
Thu Jun 10, 2021 9:34 am
Forum: Beginner's corner
Topic: Viewing Bacteria With Bright-field Microscopy
Replies: 18
Views: 338

Re: Viewing Bacteria With Bright-field Microscopy

I would be interested in this as well, Phil. There really seem to be fashions of what scientfic methods to use and this leads to the use of sub-optimal methods in some cases. As much as I like DIC as a quick method to get a visually appealing image it doesn't really bring out more detail and adds ar...
by MicroBob
Thu Jun 10, 2021 9:18 am
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 300

Re: trinocular head

About 10 years ago some people in the german forum bought stuff from India, often trino tubes. Experiences varied, overall mediocre built with much plastic and very basic threads and screws. But some experienced microscopists attested the good ones a good functionality. Here in Germany Zeiss Standar...
by MicroBob
Wed Jun 09, 2021 8:16 pm
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 300

Re: trinocular head

Hi Phil, yes, this would not be a cheap option, and I can't actually saw how good this trino tube is (tube factor, field size, split vision/photo...) There was a later model in Biolar look, the Trino Vario-Phototubus B4 with changeable split factors 100% / 30% - 70% / 100%. In the german forum a guy...
by MicroBob
Wed Jun 09, 2021 3:56 pm
Forum: Microscopes and optics
Topic: trinocular head
Replies: 11
Views: 300

Re: trinocular head

Hi Phil,
here: https://www.ebay.de/itm/292522215586
you could buy the original trino head for the MB 30.
BW Optik is a company trading in microscope stuff, partly made by themselves. As a company they should be able to ship one to you.

Bob
by MicroBob
Tue Jun 08, 2021 3:36 pm
Forum: Pictures and Videos
Topic: Slowmotion Test Footage 180-240 FPS
Replies: 4
Views: 155

Re: Slowmotion Test Footage 180-240 FPS

Hi,
nice video - what camera are you using? The resolution of my camera is fairly much reduced in it's 400 and 1200 fps modes and there is nothing in between this and 60 fps.

Here is a vorticella at 1200 fps, pixels can be count on one hand...

https://vimeo.com/263421396

Bob
by MicroBob
Mon Jun 07, 2021 7:56 pm
Forum: Microscopes and optics
Topic: OFR (OPTICS FOR RESEARCH)
Replies: 7
Views: 154

Re: OFR (OPTICS FOR RESEARCH)

Hi Steve, for normal microscopy it will probably be nothing special. But if you can assemble a complete UV transitting light path you may be able to do some quite interesting things with it. I never had a closer look at UV microscopy so perhaps you can report what you can acheive with this special p...
by MicroBob
Mon Jun 07, 2021 11:41 am
Forum: Microscopes and optics
Topic: OFR (OPTICS FOR RESEARCH)
Replies: 7
Views: 154

Re: OFR (OPTICS FOR RESEARCH)

Hi Steve,
probably the brother of this one: https://www.thorlabs.com/thorproduct.cf ... MU-10X-UVB
You will probably know when you have a use for it. It not intended for ordinarymicroscopy stuff.

Bob
by MicroBob
Sun Jun 06, 2021 5:20 am
Forum: Microscopes and optics
Topic: Is this a good objective?
Replies: 13
Views: 336

Re: Is this a good objective?

Hi Javier, the objective will be from the mid to late 80s. The knurled ring is no correction collar. There sas a series of EF achromats, "extended field" = semiplan, but this seems to be an even simpler basic achromat. It will probably be a good objective for your needs though. When looking at the a...
by MicroBob
Sun Jun 06, 2021 5:13 am
Forum: Illumination Techniques
Topic: Overhead lighting for compound scope (T720Q)
Replies: 13
Views: 273

Re: Overhead lighting for compound scope (T720Q)

Hi, for the lower powers one or two IKEA Jansjö lamps do a good job. To even out illumination a table tennis ball an be cut in half and a hold for the objective can be added.

Bob
by MicroBob
Sat Jun 05, 2021 4:59 pm
Forum: Microscopes and optics
Topic: I Have A Weight Problem
Replies: 11
Views: 289

Re: I Have A Weight Problem

Hi Jeff, I don't know you precise sitiuation but generally I would think that choosing the microscope by it's weight is an artificial limitation that will lead to a less than perfect puchase. Other than in racing bikes weight is no important property for a microscope for most users so there is littl...
by MicroBob
Sat Jun 05, 2021 4:42 pm
Forum: Introduce yourself
Topic: Germ-A-Phob
Replies: 9
Views: 345

Re: Germ-A-Phob

Hi Cruzer1100, welcome to the forum! I'm sure you will find a lot of interesting things to observe. A very rich topic to start with is water life in ponds and by scraping off pieces of plants and floating wood it is easy to collect nice material. Bacteria are not the best topic for the amateur, with...
by MicroBob
Sat Jun 05, 2021 2:14 pm
Forum: Beginner's corner
Topic: Dry fusing diatoms to cover slip.
Replies: 4
Views: 200

Re: Dry fusing diatoms to cover slip.

Hi Phill, the heating process is used for burning off organic matter to clean the diatom for better visibility of the pores. The remaining dirt keps the frustule attatched to th ecover slip well enough, so colonies of diatoms stay in formation. The cleaning will be better with higher temperatures an...
by MicroBob
Fri Jun 04, 2021 7:35 am
Forum: Beginner's corner
Topic: Advice to buy a Plan objective
Replies: 6
Views: 260

Re: Advice to buy a Plan objective

Hi Marc, to make good use of a plan objective in photography you need a camera adaptation that preserves this planarity. What kind of microscope and camera adaptation do you use and does it fit to the objective in mind? On a good microscope system plan objectives give a planar image to a field of at...
by MicroBob
Thu Jun 03, 2021 2:32 pm
Forum: Microscopes and optics
Topic: Zeiss objective info
Replies: 5
Views: 194

Re: Zeiss objective info

Hi, this is a Zeiss West dry objective for use without cover slip. It will have been in routine medical use where blood smears are usually not covered. Most medical laboratories will have moved to newer microscopes by now and for the amateur it doesn't have many uses. Perhaps one could use it for ep...
by MicroBob
Tue Jun 01, 2021 5:30 pm
Forum: My microscope
Topic: Tungsten needle for diatom manipulation.
Replies: 15
Views: 320

Re: Tungsten needle for diatom manipulation.

Hi together, tungsten carbide has some very special properties, one of them is a very high e modulus which makes it very stiff at a given section. In diamtom picking I can imagine that this can be an advantage. I used baby seal hairs for this and they are very flexible and tend to snip a frustule aw...
by MicroBob
Mon May 31, 2021 7:46 pm
Forum: Microscopes and optics
Topic: 100x Concerns
Replies: 29
Views: 1089

Re: 100x Concerns

Could you please give a brief description of how I use a Bertrand lens as well as how I buy one? Also, to view surface placement of the oil, why would I want to view the back lens and not the front lens? Hi, some microscopes (like my 1960s Zeiss Photomicroscope 1) have an included Bertrand lens, of...
by MicroBob
Sun May 30, 2021 7:20 am
Forum: Microscopes and optics
Topic: 100x Concerns
Replies: 29
Views: 1089

Re: 100x Concerns

I apply my immersion oil to the objective this way: - Slide with specimen and cover slip on sage - Focus with objective with enough free working distance to leave room for a drop - Swing nosepiece to intermediate position between 2 ports - Apply oil onto cover slip with dropper bottle or small wood ...
by MicroBob
Fri May 28, 2021 9:06 am
Forum: Pictures and Videos
Topic: Micrasterias
Replies: 4
Views: 407

Re: Micrasterias

Hi, this looks like the outer skin of a dead one to me. In my area demids are less common than they were some decades ago, but in certain places a nie varyity can be found. We have a desmid photo collection online, for comparison or just to have a look at other species: https://nwv-hamburg.de/sammlu...
by MicroBob
Thu May 27, 2021 8:27 pm
Forum: Microscopes and optics
Topic: 100x Concerns
Replies: 29
Views: 1089

Re: 100x Concerns

By gently rotating /swiping the objective into place you're less likely to have air bubbles. I have the habit to swing once into and through the oil puddle and backwards in again. This reduces the risk of trapped air under the front lens, especially with objectives that are not already oily at the ...
by MicroBob
Thu May 27, 2021 11:42 am
Forum: Microscopes and optics
Topic: First stereo scope!
Replies: 7
Views: 559

Re: First stereo scope!

Hi Ross, nice stereo microscope with classic nuclear reactor design! I have more or less the same and like it a lot. With a 0,5x aux lens the free working distance might be to long for the height of your column. There is a manual online somewhere with these distances. I use newer Olympus eyepieces w...
by MicroBob
Tue May 25, 2021 7:16 pm
Forum: Specimens, samples and slides
Topic: making longitudinal sections
Replies: 14
Views: 803

Re: making longitudinal sections

Hi, there is a very simple method: Fresh plant material contains water, which has the effect to harden cyanacrylate glue. Just prepare a block of wood and glue the fresh material onto it. When sectioning stay away from the glue with the knife as it would dull the knife quickly. Do the fixation after...
by MicroBob
Tue May 25, 2021 5:34 pm
Forum: Pictures and Videos
Topic: Flagellate from a moor - pressed out of moss
Replies: 7
Views: 359

Re: Flagellate from a moor - pressed out of moss

..Tinkered with your image in an attempt to highlight the flagellum (hope that is alright) Hi Glen, yes of cause and you were successfull too! To me it looks as if there are two flagella. The photos and videos were made on the inverted microscope with bicyle bulb illumination, the sample sandwiched...
by MicroBob
Mon May 24, 2021 4:44 pm
Forum: Resources (online, books etc.)
Topic: Micro algae database and image collection is online!
Replies: 9
Views: 1025

Re: Micro algae database and image collection is online!

Hi together, nice to hear that you like this photo collection and find it useful. The samples are from all around the world so there actually might be an algae that was found in you neighbourhood! Many of the samples were originally collected in the 1960s, some of the diatoms may have been collected...
by MicroBob
Sun May 23, 2021 7:42 am
Forum: Pictures and Videos
Topic: Flagellate from a moor - pressed out of moss
Replies: 7
Views: 359

Flagellate from a moor - pressed out of moss

Hi together,
here a photo an video of an interesting flagellate, moving around by constantly deforming it's body. It may be a Distigma proteus.
The sample is from a moor, pH ca. 4,5, pressed out of moss.

Video-link: https://vimeo.com/553665977

Bob
by MicroBob
Sat May 22, 2021 7:50 am
Forum: Microscopy accessories
Topic: Cover slip drying and storage container
Replies: 6
Views: 589

Re: Cover slip drying and storage container

One possibility is to store cover slips in alcohol and wipe them when they are taken out. This is useful when they are dirty from new on and have to be cleaned intensively anyway.
by MicroBob
Fri May 21, 2021 7:39 pm
Forum: Camera systems and imaging
Topic: eye piece projection
Replies: 12
Views: 411

Re: eye piece projection

In case someone is interested in Exakta cameras: Ivor Matanle has a chapter on them in his book: https://www.amazon.de/Collecting-Using-Classic-SLRs-Matanle/dp/0500279012 which is very interesting to read. It was a very big system and several companies made objective with Exakta fit. There also was ...
by MicroBob
Fri May 21, 2021 4:58 pm
Forum: Camera systems and imaging
Topic: eye piece projection
Replies: 12
Views: 411

Re: eye piece projection

... old school chrome on brass photo adapter base as his basic mounting infrastructure. Probably 40 or 50 years old. The adapter Rolf uses was made by Ihagee, the company that made the Exakta SLRs, one of the inventors of the SLR. Exaktas were much used in applications where a Leica rangefinder wou...