MicrobeHunter.com Microscopy Forum

Hi Brian,
one drawback of the FM31 is it's low build, not really good to use for more than minutes. Other field microscopes are much better in this area.
And when you include the necessary accessories the size of the instrument might not be so important any more.

Bob

Hi Brian, field microscopes seem to be a tiny market nowadays and at this price it is not very appealing for occasional use. Do you have a special application in mind? There are several alternatives, some quite frequently and cheaply available. When you search for fm31 or field microscope in this fo...

Hi together, a former member of our MIKRO Hamburg group spent a lot of effort in photographing and identifying micro algae. I have had his 35mm slide collection scanned and after a lot of work with connecting image and database you can find the collection on the website of our main society: https://...

Congratulations, nice sections!
The OLFA LBB blades have two bevels per side, the final micro bevels including an angle of probably 35°. So the body of the blade should be in a bit more than 17,5° to the movement plane of the block.

Bob

Nice camera adaper! It really is useful to be able to design things like this. Especially camera adaptations to microscopes can be difficult if one has to rely only on ready made parts. Before I would machine such an adapter I would just print a beefier version. This hollow stuff is fairly time cons...

Zeiss Jena and Zeiss West are close in their colour correction. There may be slight differences in the planar correction. Depending on the cameras chip size it will only cover the inner part of the image of the projective. 2.5 usually fits for full frame, 1,67 for APS-C, bigger numbers for medium or...

Hi, your setup looks good and should work well. The tube length will be over 160mm so without intermediate lenses that compensate for this 160mm objectives won't give a good image and be parfocal. Your setup could be able to provide Epi dark field but your objectives are only for bright field. Here ...

IanW wrote: ↑

Mon Apr 12, 2021 8:23 pm

kpl 3,2:1

Is this Zeiss Jena or Zeiss Oberkochen/West? KPL sounds like Zeiss West though.

IanW wrote: ↑

Mon Apr 12, 2021 8:23 pm

kpl 3,2:1

Is this Zeiss Jena or Zeiss Oberkochen/West? KPL sounds like Zeiss West though.

Hi Anton, I have a Zeiss Epiplan HD 100 /1,25 oil Epi bright/dark field objective. Illumination is by means of the Epi condenser from above, either through the objective lenses or over the ring mirror. I haven't found a use for it so far, but is for sure useful for inspection of very flat surfaces. ...

Hi together, Rolf actually uses eye- and field lens of an KPL10 low eyepoint on the barrel or a KPL 8 low eyepoint. This is only a change of distance between the lens groups. For Leitz and Olympus he ended up with a more complex lens- and barrel mix. By partially screwing in the lenses one could do ...

As far as I know he is still a dependable seller but difficult to contact. Have you tried Thilo Immel?

Also Microbob, did you mean to attach 3 photos to your last post? I don’t see them, but I’d like to! Sorry, I have added them now. I have an Olympus (FK?) projective and tested it, it is calulated for a longer camera length with a towering sepup high above the microscope's trino head. Zeiss West/Ob...

Hit together, at the moment my setup is: eye lens KPL10x barrel KPL8x base lens KPL8x eyepiece lifted 7mm, optimal use of image circle, sacrifying a little colour correction towards the border. 3 images: -some Pleurosigma with Planapo 25, oblique, left side is edge of complete image, right side is m...

Hi,
you might have a look at Rolf's new camera adaptation method: https://microscopyofnature.com/micropho ... -eyepieces

He uses combinations of standard eyepiece components to form a projective. I have tested his method and it works very well.

Bob

Thank you for posting theses nice photos!
Here is a link to a SEM-image of Melosira nummuloides: http://www.sccap.dk/pix/K-1317.jpg
It really is a very special diatom!

Bob

Hi Michael,
interesting project!
What I'm not sure about is how the focussing should be done: Moving the entire stereo microscope head back and forth or the individual optics on their inclined axis? How would the stereo image look like when focussing, natural or giving headaches?

Bob

Hi,
I'm fairly sure that these are no scratches. My guess is that you have old gummed up immersion oil on the lens, it got sticky from cleaning but wasn't removed and a bit of paper tissue got stuck.
Do you have a loupe or stereo microscope to inspect the front lenses?

Bob

Hi, nice sections and first stains! That parsley petiole is also a very nice object with a good mix of different structures. Safranine is a regresive stain when used in higher concentrations, it stains everything and you have to remove from where you don't want it. If used in very low concentrations...

Hi John, it really is not always obvious whether it was worth the effort to compose a detailed forum thread. But I think much of the effect is invisible and still very much present. Many of the more active microscopists generally post very little in forums like this, many only start to post results ...

Hi John, thank you for this interesting description! For me it is always helpful to read about your plans, decision making, observations and results. I use Leica Paraplast plus with DMSO, not because I chose it, it came to me. It melts at 56°C and shouldn't be heated above 62°C. I have no real compa...

The pair of camers will only show one image plane sharp at a time. For a good 3D- view one would need two stacked images from different angles. The typical focus drive of stereo microscopes and the inclined light pathes wil make it difficult to get a good result. As an alternative I would suggest to...

With that fluorescence condenser: Didn't you have to lower the stage? This would disturb Köhler illumination setup. And the fluorescence condenser, does it have a tube factor? I would suggest to start with one of the tested setups, right objective in the right serial number range, set up as I descri...

Hi, here is my description of the fitting components and how to set them up: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwi2rIDcy-fvAhWsgf0HHYGHAzgQFjAEegQICRAD&url=http%3A%2F%2Fwww.mikrohamburg.de%2FTips%2FTechnik%2FZEISS%2520DIC%2520alt%2520einstellen%25...

The diatoms on Kemps slide are of the bigger variety. So if you wan't to find diatoms and there are only small ones you will need a better combination of magnification and image quality. For sample collecting I would suggest to make a sieve from 25-50µ stainless mesh to be able to concentrate the sa...

Hi,
many diatoms are very small, smaller than e.g. 30µm. To see them you need a sharp image and for this a well made slide. Which microscope and objective are you using, how is your lighting set up and do you use a cover slip?

Bob

If tis doesn't work here is our new link list that includes sources for euparal: http://www.mikrohamburg.de/Linklisten/L ... 202021.pdf

Nice repair! It really is tremendously helpful to be handy with tools.

Hi, under the name "Drano" you get a can of coarse sodium hydroxyde with aluminium bits in it in Germany. This quickly cleans diatoms until they are gone. :lol: What can be used for drain as well as for diatoms is cleaner based on sodium hypochloride, fluid an smelling like chloride. In Germany sold...

Hi,
nice sections! I like the dark field image better than the phasec ontrast one. For botanic sections phase doesn't offer additional detail and adds artifacts. Next you might try staining. Wacker W3A is quick and nice.

Bob