Search found 2659 matches

by MicroBob
Sat Apr 17, 2021 7:11 am
Forum: Microscopy accessories
Topic: Disposable blades with Cambridge rocking microtome, anybody?
Replies: 14
Views: 539

Re: Disposable blades with Cambridge rocking microtome, anybody?

Congratulations, nice sections!
The OLFA LBB blades have two bevels per side, the final micro bevels including an angle of probably 35°. So the body of the blade should be in a bit more than 17,5° to the movement plane of the block.

Bob
by MicroBob
Thu Apr 15, 2021 6:11 pm
Forum: Microscopes and optics
Topic: Afocal adaptor
Replies: 4
Views: 222

Re: Afocal adaptor

Nice camera adaper! It really is useful to be able to design things like this. Especially camera adaptations to microscopes can be difficult if one has to rely only on ready made parts. Before I would machine such an adapter I would just print a beefier version. This hollow stuff is fairly time cons...
by MicroBob
Wed Apr 14, 2021 1:08 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

Zeiss Jena and Zeiss West are close in their colour correction. There may be slight differences in the planar correction. Depending on the cameras chip size it will only cover the inner part of the image of the projective. 2.5 usually fits for full frame, 1,67 for APS-C, bigger numbers for medium or...
by MicroBob
Wed Apr 14, 2021 12:21 pm
Forum: Microscopy accessories
Topic: Oil immersion lens for reflected light!
Replies: 13
Views: 347

Re: Oil immersion lens for reflected light!

Hi, your setup looks good and should work well. The tube length will be over 160mm so without intermediate lenses that compensate for this 160mm objectives won't give a good image and be parfocal. Your setup could be able to provide Epi dark field but your objectives are only for bright field. Here ...
by MicroBob
Tue Apr 13, 2021 7:21 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

IanW wrote:
Mon Apr 12, 2021 8:23 pm
kpl 3,2:1
Is this Zeiss Jena or Zeiss Oberkochen/West? KPL sounds like Zeiss West though.
by MicroBob
Tue Apr 13, 2021 7:21 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

IanW wrote:
Mon Apr 12, 2021 8:23 pm
kpl 3,2:1
Is this Zeiss Jena or Zeiss Oberkochen/West? KPL sounds like Zeiss West though.
by MicroBob
Tue Apr 13, 2021 6:00 am
Forum: Microscopy accessories
Topic: Oil immersion lens for reflected light!
Replies: 13
Views: 347

Re: Oil immersion lens for reflected light!

Hi Anton, I have a Zeiss Epiplan HD 100 /1,25 oil Epi bright/dark field objective. Illumination is by means of the Epi condenser from above, either through the objective lenses or over the ring mirror. I haven't found a use for it so far, but is for sure useful for inspection of very flat surfaces. ...
by MicroBob
Mon Apr 12, 2021 5:51 am
Forum: Camera systems and imaging
Topic: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography
Replies: 3
Views: 173

Re: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography

Hi together, Rolf actually uses eye- and field lens of an KPL10 low eyepoint on the barrel or a KPL 8 low eyepoint. This is only a change of distance between the lens groups. For Leitz and Olympus he ended up with a more complex lens- and barrel mix. By partially screwing in the lenses one could do ...
by MicroBob
Sun Apr 11, 2021 10:33 am
Forum: For forum members who want to buy and sell equipment
Topic: www.mikroskop-online.de
Replies: 2
Views: 133

Re: www.mikroskop-online.de

As far as I know he is still a dependable seller but difficult to contact. Have you tried Thilo Immel?
by MicroBob
Sun Apr 11, 2021 9:38 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

Also Microbob, did you mean to attach 3 photos to your last post? I don’t see them, but I’d like to! Sorry, I have added them now. I have an Olympus (FK?) projective and tested it, it is calulated for a longer camera length with a towering sepup high above the microscope's trino head. Zeiss West/Ob...
by MicroBob
Sat Apr 10, 2021 5:15 pm
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

Hit together, at the moment my setup is: eye lens KPL10x barrel KPL8x base lens KPL8x eyepiece lifted 7mm, optimal use of image circle, sacrifying a little colour correction towards the border. 3 images: -some Pleurosigma with Planapo 25, oblique, left side is edge of complete image, right side is m...
by MicroBob
Sat Apr 10, 2021 7:21 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 549

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

Hi,
you might have a look at Rolf's new camera adaptation method: https://microscopyofnature.com/micropho ... -eyepieces

He uses combinations of standard eyepiece components to form a projective. I have tested his method and it works very well.

Bob
by MicroBob
Fri Apr 09, 2021 12:05 pm
Forum: Pictures and Videos
Topic: Melosira nummuloides
Replies: 5
Views: 152

Re: Melosira nummuloides

Thank you for posting theses nice photos!
Here is a link to a SEM-image of Melosira nummuloides: http://www.sccap.dk/pix/K-1317.jpg
It really is a very special diatom!

Bob
by MicroBob
Thu Apr 08, 2021 6:40 pm
Forum: Beginner's corner
Topic: Using a pair of cameras with a stereo microscope
Replies: 32
Views: 1294

Re: Using a pair of cameras with a stereo microscope

Hi Michael,
interesting project!
What I'm not sure about is how the focussing should be done: Moving the entire stereo microscope head back and forth or the individual optics on their inclined axis? How would the stereo image look like when focussing, natural or giving headaches?

Bob
by MicroBob
Wed Apr 07, 2021 5:35 pm
Forum: Microscopes and optics
Topic: Could it be scratched?
Replies: 6
Views: 439

Re: Could it be scratched?

Hi,
I'm fairly sure that these are no scratches. My guess is that you have old gummed up immersion oil on the lens, it got sticky from cleaning but wasn't removed and a bit of paper tissue got stuck.
Do you have a loupe or stereo microscope to inspect the front lenses?

Bob
by MicroBob
Wed Apr 07, 2021 7:34 am
Forum: Specimens, samples and slides
Topic: My first attempt at using a hand microtome
Replies: 8
Views: 426

Re: My first attempt at using a hand microtome

Hi, nice sections and first stains! That parsley petiole is also a very nice object with a good mix of different structures. Safranine is a regresive stain when used in higher concentrations, it stains everything and you have to remove from where you don't want it. If used in very low concentrations...
by MicroBob
Wed Apr 07, 2021 5:58 am
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 28
Views: 1243

Re: Slide Making with 61°C Wax

Hi John, it really is not always obvious whether it was worth the effort to compose a detailed forum thread. But I think much of the effect is invisible and still very much present. Many of the more active microscopists generally post very little in forums like this, many only start to post results ...
by MicroBob
Tue Apr 06, 2021 7:23 pm
Forum: Specimens, samples and slides
Topic: Slide Making with 61°C Wax
Replies: 28
Views: 1243

Re: Slide Making with 61°C Wax

Hi John, thank you for this interesting description! For me it is always helpful to read about your plans, decision making, observations and results. I use Leica Paraplast plus with DMSO, not because I chose it, it came to me. It melts at 56°C and shouldn't be heated above 62°C. I have no real compa...
by MicroBob
Tue Apr 06, 2021 9:27 am
Forum: Beginner's corner
Topic: Using a pair of cameras with a stereo microscope
Replies: 32
Views: 1294

Re: Using a pair of cameras with a stereo microscope

The pair of camers will only show one image plane sharp at a time. For a good 3D- view one would need two stacked images from different angles. The typical focus drive of stereo microscopes and the inclined light pathes wil make it difficult to get a good result. As an alternative I would suggest to...
by MicroBob
Tue Apr 06, 2021 8:40 am
Forum: Microscopes and optics
Topic: Zeiss Photomicroscope DIC Inko questions.
Replies: 7
Views: 355

Re: Zeiss Photomicroscope DIC Inko questions.

With that fluorescence condenser: Didn't you have to lower the stage? This would disturb Köhler illumination setup. And the fluorescence condenser, does it have a tube factor? I would suggest to start with one of the tested setups, right objective in the right serial number range, set up as I descri...
by MicroBob
Mon Apr 05, 2021 5:09 pm
Forum: Microscopes and optics
Topic: Zeiss Photomicroscope DIC Inko questions.
Replies: 7
Views: 355

Re: Zeiss Photomicroscope DIC Inko questions.

Hi, here is my description of the fitting components and how to set them up: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwi2rIDcy-fvAhWsgf0HHYGHAzgQFjAEegQICRAD&url=http%3A%2F%2Fwww.mikrohamburg.de%2FTips%2FTechnik%2FZEISS%2520DIC%2520alt%2520einstellen%25...
by MicroBob
Mon Apr 05, 2021 7:44 am
Forum: Specimens, samples and slides
Topic: Diatom Search #2
Replies: 6
Views: 250

Re: Diatom Search #2

The diatoms on Kemps slide are of the bigger variety. So if you wan't to find diatoms and there are only small ones you will need a better combination of magnification and image quality. For sample collecting I would suggest to make a sieve from 25-50µ stainless mesh to be able to concentrate the sa...
by MicroBob
Sun Apr 04, 2021 8:14 pm
Forum: Specimens, samples and slides
Topic: Diatom Search #2
Replies: 6
Views: 250

Re: Diatom Search #2

Hi,
many diatoms are very small, smaller than e.g. 30µm. To see them you need a sharp image and for this a well made slide. Which microscope and objective are you using, how is your lighting set up and do you use a cover slip?

Bob
by MicroBob
Sun Apr 04, 2021 8:11 pm
Forum: Resources (online, books etc.)
Topic: Where to buy Euparal (in EU)?
Replies: 11
Views: 2045

Re: Where to buy Euparal (in EU)?

If tis doesn't work here is our new link list that includes sources for euparal: http://www.mikrohamburg.de/Linklisten/L ... 202021.pdf
by MicroBob
Sat Apr 03, 2021 5:10 am
Forum: Microscopes and optics
Topic: Fix the slide holder
Replies: 4
Views: 264

Re: Fix the slide holder

Nice repair! It really is tremendously helpful to be handy with tools.
by MicroBob
Sat Apr 03, 2021 5:07 am
Forum: Beginner's corner
Topic: Diatom Search
Replies: 5
Views: 204

Re: Diatom Search

Hi, under the name "Drano" you get a can of coarse sodium hydroxyde with aluminium bits in it in Germany. This quickly cleans diatoms until they are gone. :lol: What can be used for drain as well as for diatoms is cleaner based on sodium hypochloride, fluid an smelling like chloride. In Germany sold...
by MicroBob
Fri Apr 02, 2021 7:14 pm
Forum: Specimens, samples and slides
Topic: My first attempt at using a hand microtome
Replies: 8
Views: 426

Re: My first attempt at using a hand microtome

Hi,
nice sections! I like the dark field image better than the phasec ontrast one. For botanic sections phase doesn't offer additional detail and adds artifacts. Next you might try staining. Wacker W3A is quick and nice.

Bob
by MicroBob
Thu Apr 01, 2021 1:35 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 856

Re: Trying out a mono camera

Hi Louise, for diatoms these monochrome cameras are very good. Microscope optics transmit down to about 360nm. Some mountants (e.g. Pleurax) don't transmit any light below 420nm or so. If you are more interested in UV use on diatoms you might google for the work of Peter Höbel, very useful. Apart fr...
by MicroBob
Thu Apr 01, 2021 7:17 am
Forum: Sandbox
Topic: Microscopic joints and gluing
Replies: 9
Views: 706

Re: Microscopic joints and gluing

Hi,
there is a glue system based on CA glue that uses glass bubbles as a filler. It can be used to repair many things and keeps even motorcyle fairings together.
The mine are graphite and some polymer stuff so not good to glue generally.
The sculpture looks great!

Bob
by MicroBob
Wed Mar 31, 2021 7:49 pm
Forum: Introduce yourself
Topic: Hi , I'm Chris (from Paris)
Replies: 7
Views: 360

Re: Hi , I'm Chris (from Paris)

Hi Chris,
welcome to the forum!
Do you plan to convert microscope objectives to phase contrast or make phase annulli for existing objectives?

Bob