MicrobeHunter.com Microscopy Forum

My introduction to CA ‘SuperGlue’ was in the early 1970s when I worked at Kodak. It was very new then, and they used it in tiny quantities to bond the lenses into place, in the little plastic cameras. The dispensing equipment was the plumbed-in equivalent of Greg’s syringe, and the adhesive was very...

Gee MichealG that manual made me feel really dumb! Very complicated-so is it a camera that you need to be into coding and audino or raspberry PI computers etc? Or also comes with software that is user friendly? (I know hardly anything about such things-too old) ... It’s O.K. Ross ... That ‘manual’ ...

LouiseScot wrote: ↑

Mon Apr 12, 2021 8:49 pm

ps I can't see how you could use reflected light with an immersion medium which assumes transmitted light - just not possible

Not quite impossible ... they are used for Coal studies:
https://www.uj.ac.za/faculties/science/ ... raphy.aspx

MichaelG.

It shows the same effect. It looks like it's to do with that species- maybe they are quite deep? . I’ve just found this: https://www.researchgate.net/publication/314545744_Morfologia_e_distribuicao_de_Caloneis_Lyrella_Moreneis_Petroneis_e_Pinnularia_em_praias_arenosas_de_Santa_Catarina_Sul_do_Brasi...

LouiseScot wrote: ↑

Sun Apr 11, 2021 5:52 pm

Petroneis_100xAchro_vsFluorCrop50pc_Red0017.jpg
.
Louise

Lovely image from that Fluorite
... but what's with the 'staircase' effect [very evident at upper left] ?

MichaelG.

MicroBob wrote: ↑

Sun Apr 11, 2021 9:38 am

Zeiss West/Oberkochen normally had an adaptation using an 63mm achromat in the light path to the camera […]

@ScienceMatters ... You might find this thread worth a look:
https://www.microbehunter.com/microscop ... f=5&t=7871

MichaelG.

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What an excellent page that is, Bob !!

Thanks for the link

MichaelG.

I found the data-sheet for the sensor used in the DN3UVC-130
https://www.teledyne-e2v.com/content/up ... 76C560.pdf
... may be of interest [or not]

MichaelG.

. The brief tests I did with the Arducams were on a Raspberry Pi 4 ... I could never get them to work on either of my Macs. One USB 3 channel was always a little slower than the other, whether running MPEG4 or YUYV ... I didn’t investigate further, because I had decided to return them. The Japanese ...

A pair of these cameras might be just what I need for 'real-time' stereo:
https://www.shodensha-inc.co.jp/en/dn3uvc-130/

Has anyone dealt with Shodensha ?

MichaelG.

Slightly off-topic ... but I did mention ViewMaster

Here’s a serious scientific application:
https://www.ebay.co.uk/itm/1987-2-Vol-S ... 3204513224

MichaelG.

.

I’ve just found the video to accompany the smartphone article:
https://static-content.springer.com/esm ... M3_ESM.mp4

[ not for the squeamish ]

MichaelG

That’s a very significant question, Bob

I sincerely hope that it is the former !

MichaelG.

As promised ... a few brief notes: I have been interested in stereoscopes for many years, and have a quite unreasonable fondness for the ViewMaster system ... which has all of the magic, but very little of the image quality, that we are seeking. https://www.viewmaster.co.uk/htm/quickref.asp The idea...

MichealG here’s an interesting link I just found basically talking about the iPhone idea for making 3-D medical training videos from surgical stereo microscopes! Now I’m really going down a rabbit hole! https://www.nature.com/articles/eye2015282.pdf . Many thanks for that link, Ross ... This is the...

Scarodactyl wrote: ↑

Wed Apr 07, 2021 3:46 pm

[…] That would probably be the most convenient method for video, though not the cheapest.

Thanks ... but even with that generous discount, way out of my price range for this.

MichaelG.

. Great post, Rossf ... thanks My own interest is perhaps more ‘technical’ than your educational sessions, but we’re certainly on similar paths. ... reasonably high resolution and high frame-rate video is important for what I want; hence the interest in Holmes stereoscopes and their modern equivalen...

The pair of camers will only show one image plane sharp at a time. For a good 3D- view one would need two stacked images from different angles. The typical focus drive of stereo microscopes and the inclined light pathes wil make it difficult to get a good result. I’m not sure what mgualt had in min...

I have absolutely no experience with Amscope products, but this looks a reasonable set-up tutorial:
https://youtu.be/coD33VcSL5A

MichaelG.

Gotcha. Reading the user manual I am now even more perplexed. Looking at the screenshots for the Windows/Mac software, it appears the Windows version has more tooling :cry: :evil: Unfortunately ... that is quite a common state of affairs ToupLite for example is the ‘poor relation’ of ToupView http:...

Here’s one of my test shots:
. .

MichaelG.

Hello, I would like to try taking stereoscopic photos using a stereo microscope. My guess is the easiest way to do this would be to get a good stereo microscope and replace both eyepieces with cameras. I'm wondering if anyone has tried this, and also if there is a stereo microscope with really good...

I have no idea what the Zeiss standard of accuracy was. Well the Germans are known for their engineering standards and attention to details so I imagine fairly tight tolerances. On which basis ... I still have no idea what the Zeiss standard of accuracy was :? O.K. we can imagine “not rubbish” ... ...

Thanks

... That certainly gives you a ‘stretch target’

MichaelG.

^^^
Broken link there, Louise

You are already getting close to the best image here:
https://www.algaebase.org/search/specie ... s_id=34118

Keep up the great work

MichaelG.

Thanks from me too, synnus

I have downloaded the document just for technical interest, and the introductory chapter looks very helpful.

MichaelG.

That’s looking very good

MichaelG.

The classic way is to use a lathe ... centre one element on a ‘wax chuck’, add warm balsam and the second element, and push it gently into place whilst it’s rotating. Sounds easy ... :roll: I have no idea what the Zeiss standard of accuracy was. MichaelG. . Edit: __ and here’s a more modern system; ...

Wes wrote: ↑

Fri Apr 02, 2021 9:03 pm

The ultimate way to fix delaminated eyepieces would be to soak the doublets in hexane and once they separate apply fresh lens cement.

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Assuming that you have the equipment/skill to centre the two elements to the original standard.

MichaelG.