Search found 3309 matches

by Hobbyst46
Mon Apr 19, 2021 11:10 am
Forum: Pictures and Videos
Topic: Actinocyclus octonarius
Replies: 9
Views: 123

Re: Actinocyclus octonarius

very attractive image !
Is the 12.5X KPL raised above parfocality height ?
Is it a 12.5x -W high eyepoint KPL ?
by Hobbyst46
Wed Apr 14, 2021 9:33 pm
Forum: Illumination Techniques
Topic: Tryblionella under darkfield and oblique darkfield
Replies: 1
Views: 86

Tryblionella under darkfield and oblique darkfield

This freshwater Tryblionella gracilis (or Nitzchia Tryblionella), length 109um, is poorly resolved under light microscopy. Even in Pleurax. In the oblique image (photo 1) contrast was enhanced. Illumination was amber-filtered cool LED. Photo 2 is plain darkfield, with unfiltered cool LED light. Turr...
by Hobbyst46
Tue Apr 13, 2021 5:03 pm
Forum: Beginner's corner
Topic: Very dirty Nikon microscope, what to do?
Replies: 10
Views: 311

Re: Very dirty Nikon microscope, what to do?

Some clarifications. 70% ethanol is a proper disinfection liquid, not 95% or 100% ethanol. I suggested it in the case of fear from any microbes that were attached to the scope. Not a must. If the outside of the microscope is stained with grease or oil or some sticky organic residue, try one of the s...
by Hobbyst46
Tue Apr 13, 2021 10:23 am
Forum: Beginner's corner
Topic: Very dirty Nikon microscope, what to do?
Replies: 10
Views: 311

Re: Very dirty Nikon microscope, what to do?

There are several issues here that are better treated separately, one by one. It would help to see photos of the microscope. And initial treatment and cleaning should be done without any disassembly. Deep clean, complete tear down is not the first step to take. 1. To Return or not to Return. It depe...
by Hobbyst46
Mon Apr 12, 2021 9:51 pm
Forum: My microscope
Topic: Buying 3rd Party Eyepieces for stereo scope
Replies: 4
Views: 90

Re: Buying 3rd Party Eyepieces for stereo scope

I bought third party 20x/12 WF eyepieces for my Olympus stereo. They work, but I never use them. Very inconvenient. Vision seems unnatural. I believe that 10x WF are OK and 15x might be fine; 20x is too much. For higher magnifications, an 1.5x Barlow lens would be better. Just my opinion.
by Hobbyst46
Mon Apr 12, 2021 3:43 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

Wow! So many you have their Bilbo! So is that actually a Petroneis sideways on? I'd say it was more like 20um just measuring with a ruler on screen. So 20x60 isn't far removed from 30x70. Anyway, I'm still not sure of the causes of the image aberrations above... Yes, it is sideways on. I flipped it...
by Hobbyst46
Mon Apr 12, 2021 2:40 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

The source gives length, width and height - good enough?A 'thickness' of 30um seems quite a lot when width is also 30um I stand corrected, sorry ! So here is the measurement. Recorded with the 16x0.32 Plan objective. Photo 1 shows the LED strip stand. Laid on the microscope stage (usually the stere...
by Hobbyst46
Mon Apr 12, 2021 1:52 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

Thickness - neither width nor height.
by Hobbyst46
Mon Apr 12, 2021 1:32 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

It shows the same effect. It looks like it's to do with that species- maybe they are quite deep? . I’ve just found this: https://www.researchgate.net/publication/314545744_Morfologia_e_distribuicao_de_Caloneis_Lyrella_Moreneis_Petroneis_e_Pinnularia_em_praias_arenosas_de_Santa_Catarina_Sul_do_Brasi...
by Hobbyst46
Sun Apr 11, 2021 8:42 pm
Forum: Camera systems and imaging
Topic: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography
Replies: 3
Views: 175

Re: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography

PeteM wrote:
Sun Apr 11, 2021 8:31 pm
That looks to be a very good result. Thanks for sharing it.

Do the Zeiss KPL 8x lower half and 10x upper half simply screw together for the spacing you used?
Yes, they do. Both are Huygens type, I believe, and belong in the same "series".
by Hobbyst46
Sun Apr 11, 2021 7:06 pm
Forum: Camera systems and imaging
Topic: "Hybrid" 160mm TL Zeiss KPL eyepiece projection photography
Replies: 3
Views: 175

"Hybrid" 160mm TL Zeiss KPL eyepiece projection photography

Very recent experiments on "hybrid" Zeiss eyepiece projection photography with my 160 TL Zeiss microscope. I was motivated by the work of Rolf Vossen ("Microphotography with ‘hybrid’ eyepieces", https://microscopyofnature.com/experiments-and-tests), and exchange of ideas with Bob (MicroBob). My proj...
by Hobbyst46
Sun Apr 11, 2021 6:08 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

Getting better all the time !
by Hobbyst46
Sun Apr 11, 2021 4:25 pm
Forum: Pictures and Videos
Topic: Trying out my new objective
Replies: 26
Views: 319

Re: Trying out my new objective

Very lovely high resolution images. Once again demonstrate immersion power. ;) Oblique, aren't they ? how did you get the oblique illumination ? Since that is a Nikon Cf, would be interesting to see color images as well. Incidentally, is that a Fluorite objective ? the CF and F do not mean fluorite ...
by Hobbyst46
Sat Apr 10, 2021 9:18 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Zeiss KPL 2.5x Photo Eyepiece
Replies: 17
Views: 555

Re: Wanted: Zeiss KPL 2.5x Photo Eyepiece

MichaelG. wrote:
Sat Apr 10, 2021 9:02 am
.

What an excellent page that is, Bob !!

Thanks for the link

MichaelG.
The modified eyepiece idea as shown by Rolf Vossen works well on my setup too.
by Hobbyst46
Fri Apr 09, 2021 11:52 am
Forum: Pictures and Videos
Topic: Licmophora abbreviata
Replies: 9
Views: 263

Re: Licmophora abbreviata

Very beautiful Melosira and especially Licmophora.
by Hobbyst46
Tue Apr 06, 2021 6:43 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

Images 014, 023 and 017 nicely show the different structural details of the diatom. Like, ridges on the inner layer and round holes on the outer (according to the SEM images in the recently linked article).
by Hobbyst46
Tue Apr 06, 2021 4:29 pm
Forum: Identification help
Topic: Diatom id?
Replies: 6
Views: 220

Re: Diatom id?

I think that the main differences between stacking softwares is speed, efficiency, user friendliness etc rather than the quality of the final product. My preferred stacking program is Toupview (free I think). Tab "Process"->"EDF" (extended depth of focus). Superb software in general. Here is a stack...
by Hobbyst46
Tue Apr 06, 2021 3:34 pm
Forum: Identification help
Topic: Diatom id?
Replies: 6
Views: 220

Re: Diatom id?

The excellent resolved stria pattern of image "Do415nmNikon40xCrop100pcScale_00013" is not visible in the stacks. My opinion is that for this diatom, to choose the few images that manifest well focused surfaces and stack them. So I did for a twin of this diatom, albeit I had phase contrast images.
by Hobbyst46
Tue Apr 06, 2021 12:58 pm
Forum: Identification help
Topic: Diatom id?
Replies: 6
Views: 220

Re: Diatom id?

A guess - an Amphora species.
by Hobbyst46
Tue Apr 06, 2021 8:04 am
Forum: Illumination Techniques
Topic: What am i doing wrong?
Replies: 11
Views: 468

Re: What am i doing wrong?

Type B immersion oil is highly viscous whether new or old. Type A is much less viscous and serves well.
by Hobbyst46
Mon Apr 05, 2021 9:50 pm
Forum: Illumination Techniques
Topic: What am i doing wrong?
Replies: 11
Views: 468

Re: What am i doing wrong?

Perhaps it is designed to be oiled to the bottom of the slide. with immersion oil.
by Hobbyst46
Sat Apr 03, 2021 5:03 pm
Forum: Specimens, samples and slides
Topic: Alboran Sea Diatoms
Replies: 11
Views: 380

Re: Alboran Sea Diatoms

Fantastic diversity. From which depth (in the mud, and below the water) was the sand collected ? I used a jam jar to scoop the wet sand from the surface down to 2 - 3 cm at low water. You might find this link useful. You can set it for your area https://www.tidetime.org/europe/spain/estepona.htm Th...
by Hobbyst46
Sat Apr 03, 2021 3:02 pm
Forum: Specimens, samples and slides
Topic: Alboran Sea Diatoms
Replies: 11
Views: 380

Re: Alboran Sea Diatoms

Fantastic diversity.
From which depth (in the mud, and below the water) was the sand collected ?
It is great that you do not boil in acid any more.
by Hobbyst46
Sat Apr 03, 2021 10:13 am
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

The linked article nicely demonstrates the structural difference between the inner and outer surfaces of the valves (ribs vs dots, say). Can we see this difference with (home) light microscopy, rather than SEM ? These diatoms on the slide were purposely mildly cleaned, without resort to harsh chemic...
by Hobbyst46
Fri Apr 02, 2021 9:41 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

{ Speedax} It might not actually be available yet. Have a look here: https://www.tmg-tuebingen.de/neues-hochbrechendes-einschlussmittel-speedax/ I'm not sure if there's any connection, but I've previously come across a few high refractive index resins made by doping with nanoparticles. Will be inte...
by Hobbyst46
Fri Apr 02, 2021 8:09 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

The UV image is indeed very good. For example, here is another example of that diatom (Petroneis humerosa) imaged under phase contrast, 100X1.3 oil objective, and blue light (~450nm). The UV image is better resolved, although there are other variables.
by Hobbyst46
Thu Apr 01, 2021 10:11 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

The 486nm light has improved the image. Certainly a powerful LED will be better than filtration of white light. I cannot stand the blue light in my eyes though. Is it easy to switch the LEDs on the Swift microscope, from white to blue? On my microscope it would need fabrication of a new LED illumin...
by Hobbyst46
Thu Apr 01, 2021 9:22 pm
Forum: Illumination Techniques
Topic: What oil is best?
Replies: 2
Views: 198

Re: What oil is best?

Greg Howald wrote:
Thu Apr 01, 2021 9:18 pm
I've been using mineral oil at 100x with success but I just ordered an oil dark field condenser and a 100x objective with iris. What oil is best for this?
Thanks,
Greg
Plain good old immersion oil, for example, Cargille Type A. Sold under various names.
by Hobbyst46
Thu Apr 01, 2021 9:20 pm
Forum: Camera systems and imaging
Topic: Trying out a mono camera
Replies: 29
Views: 873

Re: Trying out a mono camera

The 486nm light has improved the image. Certainly a powerful LED will be better than filtration of white light. I cannot stand the blue light in my eyes though. Is it easy to switch the LEDs on the Swift microscope, from white to blue? On my microscope it would need fabrication of a new LED illumina...