Search found 4084 matches

by mrsonchus
Thu Mar 04, 2021 12:18 pm
Forum: Important announcements
Topic: This forum is now more secure
Replies: 8
Views: 744

Re: This forum is now more secure

Good news, many thanks!
by mrsonchus
Wed Mar 03, 2021 9:56 pm
Forum: Specimens, samples and slides
Topic: Lichen Squash
Replies: 1
Views: 239

Lichen Squash

Hi all, some may remember the recent adventure of the lichens I posted. This resulted in the production of some pretty nice permanent lichen-section slides (of the species Xanthia.parietina as it transpired). Well, as I was tidying-up in the lab today I placed some lichens into their dry storage jar...
by mrsonchus
Tue Mar 02, 2021 11:23 pm
Forum: Digital processing
Topic: rotating cylinder to surface
Replies: 15
Views: 1461

Re: rotating cylinder to surface

Perhaps a video of the turning cylinder may be stitched to a panorama of the video frames, which would give you what you need? Video-scan stitching is very fast - the software does it all. I use this routinely to image whole specimens on slides rather than take dozens of stills manually. The results...
by mrsonchus
Tue Mar 02, 2021 12:24 am
Forum: Pictures and Videos
Topic: More from the aquarium
Replies: 2
Views: 303

Re: More from the aquarium

Interesting video - almost looked like 'he' paused when he realised he was being filmed!
by mrsonchus
Mon Mar 01, 2021 1:11 pm
Forum: Microscopes and optics
Topic: I don't really understand these objectives
Replies: 3
Views: 382

Re: I don't really understand these objectives

Hi Greg, these sorts of paradoxes are a real puzzle! Interested to see any responses as I'd like to know more also, and I bet many others would too.
by mrsonchus
Mon Mar 01, 2021 1:08 pm
Forum: Important announcements
Topic: Updated Forum Rules
Replies: 4
Views: 11957

Re: Updated Forum Rules

Great move Oliver, the World's in danger of toxic-overflow it seems!
by mrsonchus
Mon Mar 01, 2021 10:38 am
Forum: Identification help
Topic: Is this identification correct?
Replies: 5
Views: 416

Re: Is this identification correct?

Looks good!
I think your images are pretty good, I wouldn't worry about your camera it looks good enough to me.
by mrsonchus
Mon Mar 01, 2021 12:58 am
Forum: Identification help
Topic: Is this identification correct?
Replies: 5
Views: 416

Re: Is this identification correct?

Hi, it's quite a bit too magnified to tell really. Need to see the whole section to be able to see surrounding tissues and to know what organ/tissue/part-of organ this is.... I'd guess that the epidermis is correct but the collenchyma could do with a little more detail - may be right though. Any mor...
by mrsonchus
Sun Feb 28, 2021 4:44 pm
Forum: Specimens, samples and slides
Topic: I bought a set.
Replies: 10
Views: 929

Re: I bought a set.

Hmm, sorry to hear that, good ones are a bit scarce it seems.
I have hundreds of Botanical permanent slides that I could sell a set from, but I doubt if I could match the cost of the cheapest ones I'm afraid.
by mrsonchus
Sun Feb 28, 2021 4:37 pm
Forum: Specimens, samples and slides
Topic: Will these melt paraffin wax?
Replies: 9
Views: 953

Re: Will these melt paraffin wax?

Hi, yes wax will dissolve/melt in xylene or a limonene-based agent such as the Histoclear that I use.
But, this is only the wax antemedium, for infiltration prior to embedding then sectioning pure molten wax has to be used...
by mrsonchus
Sun Feb 28, 2021 3:54 pm
Forum: Microscopy accessories
Topic: Disposable blades with Cambridge rocking microtome, anybody?
Replies: 14
Views: 1061

Re: Disposable blades with Cambridge rocking microtome, anybody?

Have a look at my old post re this, the knife itself coul be used as the basis of a holder- drilled-holes, thin metal strip to clamb and a maybe a solution - on this post cellotape works!

Here's the start of this experiment -
by mrsonchus
Sun Feb 28, 2021 11:48 am
Forum: Microscopy accessories
Topic: Disposable blades with Cambridge rocking microtome, anybody?
Replies: 14
Views: 1061

Re: Disposable blades with Cambridge rocking microtome, anybody?

Aha! Things have changed since I posted re this excellent little 'tome. With the ability now to 3D print, and in a choice of materials with differing physical properties including strength, hardness, stiffness etc I suspect that the problem has an affordable solution. The principle of a disposable-b...
by mrsonchus
Wed Feb 24, 2021 5:50 pm
Forum: Pictures and Videos
Topic: Hi sweetie, I've been looking for you!
Replies: 5
Views: 451

Re: Hi sweetie, I've been looking for you!

Must agree, beautiful, detailed and bright image!
by mrsonchus
Tue Feb 23, 2021 8:28 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

John, unfortunately scuddlebut do not ship to Canada or the US due to some insurance issues however I have been able to source the Fast Green powder from a US supplier now. As far as these stains being sometimes supplied dissolved in Cellosolve, is that an impediment for botanic staining? Thanks mi...
by mrsonchus
Tue Feb 23, 2021 12:25 am
Forum: Camera systems and imaging
Topic: Are photo eyepieces needed?
Replies: 25
Views: 3701

Re: Are photo eyepieces needed?

Hi, the optics don't project pixels remember, optical enlargement to cover more of a sensor with the image from an eyepiece or photo-eyepiece or intermediate optic will still be 'using' the full pixel-count of the sensor? I think it's the digital enlargement that adds no pixels merely interpolating ...
by mrsonchus
Mon Feb 22, 2021 7:22 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Hi all, yes as Bob says, I've been buying my dyes from the same superb supplier - scuddlebut AKA Magnacol , for about the last 5 years. I began with Brunel Microscopes and as I learned more I naturally widened my scope for a growing selection of lab requirements. This is the fellow I buy virtually a...
by mrsonchus
Mon Feb 22, 2021 4:49 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Hi Bob, thanks for the kind comments! My go-to working solution of safranin is 1% aqueous. The aqueous stains tend to be pretty simple to use in general I'd say. Addition of a counter-stain, or even a third and occasionally even a fourth to the tissue is where the complications begin, and the learni...
by mrsonchus
Sun Feb 21, 2021 11:31 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Hi, I cut some new 2µ sections with the blocks chilled in the deep-freeze, which hardened the wax-blocks considerably and helped a lot with sectioning at the 2µ level. Still there was a measure of folding where the tissue was I think harder than the wax (I need to buy some more that has a 58 deg mp,...
by mrsonchus
Sun Feb 21, 2021 10:34 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Thanks hobby and kurt, pleased you like them!
by mrsonchus
Sat Feb 20, 2021 11:55 am
Forum: Miscellaneous
Topic: I got tossed.....
Replies: 6
Views: 694

Re: I got tossed.....

Hi Paul, yes I bet we've all had this at some tome, makes you feel like gnawing your own arm off! My std practice now is to 'duplicate tab' as I go, sticking to the leftmost tab of the edit and simply making a new duplicate when I think I've added new content that I don't want to lose and reapeat al...
by mrsonchus
Fri Feb 19, 2021 2:56 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Many thanks for your kind comments chaps, really pleased you like the adventure.

Apo' - it surprised me too to see just what lichens are and how they're 'built' as it were!
It's very nice to receivce such kind compliments, thanks all. :) :D
by mrsonchus
Fri Feb 19, 2021 12:06 am
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Crikey, things just became even more fascinating! I looked back over some hand-sectioned/dissected lichen I had under the 'scope back in January - I didn't make permanent slides back then, but I did take images. This lichen I think was a common 'jelly lichen' called 'Collema.fasciculare' and was gro...
by mrsonchus
Thu Feb 18, 2021 11:24 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Re: Final Lichen Images - Apothecia!

Many thanks, pleased you like them. I've been having a look on t'internet for lichen spore form info and think I may have found some interesting info with images at this fine website .... I thought at the outset of this adventure that the lichen featured here may be the common foliose-lichen called ...
by mrsonchus
Thu Feb 18, 2021 10:18 pm
Forum: Specimens, samples and slides
Topic: Final Lichen Images - Apothecia!
Replies: 18
Views: 1508

Final Lichen Images - Apothecia!

Hi all, if you've seen my previous posts on the adventure of making Lichen slides you may remember that I was hoping to make some 2µ sections having already cut 10µ then 4µ sections. With a view to seeing if they'd section at 2µ and if any extra detail may be seen I tried to section at 2µ. Well, the...
by mrsonchus
Wed Feb 17, 2021 10:04 pm
Forum: Microscopes and optics
Topic: Stereo monovision
Replies: 13
Views: 853

Re: Stereo monovision

Just a quick but probably wrong thought. Have you actually set the 'scope to be parfocal in the required way for a stereo-zoom. 1) set both diopter adjustments to zero - i.e. no adjustment applied. 2) zoom to max-zoom and focus as sharply as possible on an object, edge whatever, using both eyepieces...
by mrsonchus
Wed Feb 17, 2021 12:05 am
Forum: Specimens, samples and slides
Topic: To embed or not to embed ?
Replies: 22
Views: 2070

Re: To embed or not to embed ?

Hi Bob, most welcome. The PDF I downloaded of the book is very handy as you say on a tablet. I must also say that the scans on your linked-to site are of a very high quality. Many thanks for this very handy link Bob.
by mrsonchus
Tue Feb 16, 2021 8:19 pm
Forum: Specimens, samples and slides
Topic: To embed or not to embed ?
Replies: 22
Views: 2070

Re: To embed or not to embed ?

John, thank you so much! It means a lot to get a compliment from you regarding a plant section photo, your threads are the ones that I looked at for guidance on how to hand section very well!! :D It is like getting an autograph from a favorite celebrity. Yes, the pictures are of an emergent petiole...
by mrsonchus
Mon Feb 15, 2021 7:37 pm
Forum: Specimens, samples and slides
Topic: To embed or not to embed ?
Replies: 22
Views: 2070

Re: To embed or not to embed ?

I like the last two images, seems to be an emergent petiole just beginning to expand it's lateral meristem output to build a lamina, excellent! Here's an example of a safranin-stained hand section I made a long while ago and is in this post of mine from 2019... The different shades are all from a si...
by mrsonchus
Mon Feb 15, 2021 7:35 pm
Forum: Specimens, samples and slides
Topic: To embed or not to embed ?
Replies: 22
Views: 2070

Re: To embed or not to embed ?

Exactly what I say - hand-sectioning is so accessible and enjoyable, especially as now it's so easy and cheap to take images for later study and comparisons etc! A really good aqueous stain for hand sectioning is toluidine-blue, used at about 0.05%. It's easy to get, cheap and totally reliable. As a...